rS. equi subsp. zooepidemicus IgG endopeptidase IdeZ, CF

R&D Systems, part of Bio-Techne | Catalog # 11572-IZ

Recombinant Streptococcus His-tag
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11572-IZ-050
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Key Product Details

  • R&D Systems E. coli-derived rS. equi subsp. zooepidemicus IgG endopeptidase IdeZ (11572-IZ)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession #

WP_014622780.1

Conjugate

Unconjugated

Applications

Enzyme Activity

View all IdeZ Proteins and Enzymes »

Product Specifications

Source

E. coli-derived IdeZ protein
Asp35-Ser349, with an N-terminal Met and C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

36 kDa

SDS-PAGE

35-39 kDa, under reducing conditions

Activity

Measured by its ability to cleave Human IgG.
The DC50 is <25 ng, as measured under the described conditions. The DC50 is defined as the amount of enzyme required to cleave 50% of 1 μg human IgG in 30 minutes at 37 °C. Use of Recombinant Streptococcus equi subsp. zooepidemicus IdeZ in the cleavage of other IgGs may required alternative conditions for optimal performance.

Scientific Data Images for rS. equi subsp. zooepidemicus IgG endopeptidase IdeZ, CF

Recombinant Streptococcus equi subsp. zooepidemicus IgG endopeptidase IdeZ His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Streptococcus equi subsp. zooepidemicus IgG endopeptidase IdeZ His-tag Protein (Catalog # 11572-IZ) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-39 kDa, under reducing conditions.

Formulation, Preparation and Storage

11572-IZ
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: IdeZ

IdeZ from bacterial pathogen S. equi subspecies zooepidemicus, a group C streptococci common in animal infection, is a cysteine protease IgG endopeptidase that acts to ablate immune effector function during infection. As a homolog of the better-known IdeS/MAC from S. pyogenes, IdeZ also contains conserved critical residues that allow it to recognize and cleave at a single site below the hinge of IgG to produce F(ab')2 and Fc fragments with high specificity (1,2). However, IdeZ contains only a single cysteine and can cleave IgG more efficiently under non-reducing conditions (2). The bacterial pathogen is known to have a broad host range and since it is capable of cleaving IgG from multiple species it can cause pathogenesis in many animals such as strangles in horses (1), hemorrhagic pneumonia in dogs in kennels (3), and mastitis in ruminates on farms (4). S. zooepidemicus human infection has been reported from direct exposure to infected animals or contracted from infected animal products resulting in nephritis, hospitalization and death as IdeZ is capable of efficient cleavage of all human IgGs (5-8).  IdeZ has been reported as a tool in analytical strategies to characterize therapeutic antibodies (9,10) and to cleave proteins post-translationally modified with O-GlcNAc to enable immunoprecipitation analysis (11). IdeZ's broader specificity compared to IdeS means it can more effectively be used in animal model studies, for example due to its ability to more effectively cleave mouse IgG2a and IgG3. IdeZ has also been proposed as a target to develop vaccines to protect animals against group C streptococci (12). Finally, IdeZ has been used in the development of recombinant adeno-associated virus (AAV) vectors for the genetic therapy of rare diseases to eliminate already existing circulating neutralizing antibodies against recombinant AAVs (13). 

References

  1. Boksha, I.S. et. al. (2023) Biochemistry 88:731.
  2. Lannergard, J. and B. Guss (2006) FEMS Microbiol. Lett. 262:230.
  3. Priestnall, S. and K. Erles (2011) Vet. J. 188:142.
  4. Pisoni, G. et. al. (2009) J. Dairy Sci.92:943.
  5. Bradley, S.F. et. al. (1991) Rev. Infect. Diseases 13:270.
  6. Balter, S. et. al. (2000) Lancet. 355:1776.
  7. Abbott, Y. et. al. (2010) J. Med. Microbiol. 59:120.
  8. Pelkonen, S. et. al. (2013) Emerg. Infect. Dis. 19:1041.
  9. Bobaly, B. et. al. (2017) J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.1060:325.
  10. Ruppen, I. et. al. (2023) J. Pharm. Biomed. Anal. 236:115743. 
  11. Machacek, M. et. al. (2020) Anal. Biochem. 611:114001.
  12. Velineni, S. and J.F. Timoney (2013) Vaccine. 31:4129.
  13. Elmore, Z.C. et. al. (2020) JCI Insight 5:e139881.

Long Name

rS. equi subsp. zooepidemicus IgG endopeptidase IdeZ

Alternate Names

ideE, IgG endopeptidase

UniProt

WP_014622780.1

Additional IdeZ Products

Product Documents for rS. equi subsp. zooepidemicus IgG endopeptidase IdeZ, CF

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Product Specific Notices for rS. equi subsp. zooepidemicus IgG endopeptidase IdeZ, CF

For research use only

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