Recombinant Viral CCI Fc Chimera (CHO-expressed) Protein

R&D Systems, part of Bio-Techne | Catalog # 7055-CC

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Carrier Free
7055-CC-025/CF
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With Carrier
7055-CC-025
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Key Product Details

  • R&D Systems CHO-derived Recombinant Viral CCI Fc Chimera (CHO-expressed) Protein (7055-CC)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession #

P19063

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CCI Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived viral CCI protein
Viral CCI
(Met1-Val258)
Accession # P19063		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met17

Predicted Molecular Mass

52.6 kDa (monomer)

SDS-PAGE

60-66 kDa, reducing conditions

Activity

Measured by its ability to inhibit JE-induced chemotaxis of BaF3 mouse pro-B cells transfected with human CCR2A.
The ED50 for this effect is 0.06-0.3 μg/mL in the presence of 20 ng/mL of Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE).

Scientific Data Images for Recombinant Viral CCI Fc Chimera (CHO-expressed) Protein

Recombinant Viral CCI Fc Chimera (CHO-expressed) Protein Bioactivity

Recombinant Viral CCI Fc Chimera (CHO-expressed) Protein Bioactivity

Measured by its ability to inhibit JE-induced chemotaxis of BaF3 mouse proB cells transfected with human CCR2A. The ED50 for this effect is 0.06 -0.3 µg/mL in the presence of 20 ng/mL of Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE).

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 7055-CC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 7055-CC/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCI

The family of T1/35 kDa proteins are encoded by the '35K' virulence gene of many poxviruses and are secreted from virus-infected cells. These proteins bind
CC-chemokines with high affinity and are termed viral chemokine inhibitor (vCCI). Viral CCI from various poxviruses share multiple stretches of sequence identity and eight conserved cysteine residues (1). The vaccinia virus (strain Lister) vCCI cDNA encodes a 258 amino acid (aa) protein with a putative 17 aa signal peptide (2). Vaccinia virus (strain Lister) vCCI shows greater than 90% aa sequence identity with vCCI from other orthopoxviruses and approximately 40% aa sequence identity with the leporipoxvirus T-1 proteins. vCCI binds with high affinity to many human, mouse, and rat CC-chemokines (1, 3-5). It binds to the same surfaces of these chemokines that are involved in chemokine receptor interactions (4-7). Some CC-chemokines utilize common binding surfaces on vCCI, while others interact with distinct sites on vCCI (8). vCCI inhibits CC-chemokine induced monocyte chemotaxis in vitro but does not block cellular effects induced by CXCL5/ENA-78, CXCL8/IL-8, or CXCL10/IP-10 (3-5). In vivo, vCCI restricts the infiltration of  immune cells into sites of inflammation (3, 9, 10) and also the CC-chemokine induced migration of arterial vascular smooth muscle cells (11).

References

  1. Graham, K.A. et al. (1997) Virology 229:12.
  2. Patel, A.H. et al. (1990) J. Gen. Virol. 71:2013.
  3. Buatois, V. et al. (2010) J. Immunol. 185:2544.
  4. Smith, C.A. et al. (1997) Virology 236:316.
  5. Lalani, A.S. et al. (1998) Virology 250:173.
  6. Beck, C.G. et al. (2001) J. Biol. Chem. 276:43270.
  7. Seet, B.T. et al. (2001) Proc. Natl. Acad. Sci. 98:9008.
  8. Burns, J.M. et al. (2002) J. Biol. Chem. 277:2785.
  9. Dabbagh, K. et al. (2000) J. Immunol. 165:3418.
  10. Reading, P.C. et al. (2003) J. Immunol. 170:1435.
  11. Spinetti, G. et al. (2004) Arterioscler. Throm. Vasc. Biol. 24:1397.

Long Name

CC Chemokine Inhibitor

UniProt

P19063 (Viral)

Additional CCI Products

Product Documents for Recombinant Viral CCI Fc Chimera (CHO-expressed) Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS 7055-CC (with carrier)
Download SDS 7055-CC/CF (carrier free)

Product Specific Notices for Recombinant Viral CCI Fc Chimera (CHO-expressed) Protein

For research use only

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