Recombinant Rhesus Macaque IL-12 Protein

Name: Recombinant Rhesus Macaque IL-12 Protein
Brand: R&D Systems
SKU: 3216-RL

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3216-RL

Citations (3)

Product Datasheet / COA / SDS

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Setting the standard in quality research reagents

Key Product Details

Source:	CHO
Accession #:	P48095 (p40) & NP_001038199 (p35)
Structure / Form:	Disulfide-linked heterodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Rhesus Macaque IL-12 Protein

Product Specifications for Recombinant Rhesus Macaque IL-12 Protein

Source

Chinese Hamster Ovary cell line, CHO-derived rhesus macaque IL-12 protein

Rhesus Macaque IL-12 (p40) (Ile23 - Ser328) Accession # P48095	6-His tag
Rhesus Macaque IL-12 (p35) (Arg57 - Ser253) Accession # NP_001038199	6-His tag
N-terminus	C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ile23 (p40) & Arg57 (p35)

Predicted Molecular Mass

35.6 kDa (p40), 23.4 kDa (p35)

SDS-PAGE

40-45 kDa and 36-37 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED₅₀ for this effect is 0.07-0.3 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

3216-RL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3216-RL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-12

Interleukin 12 (IL-12) is a 70 - 75 kDa heterodimeric glycoprotein that belongs to the IL-12 family of cytokines (1 - 3). Monkey IL-12 consists of two disulfide-linked subunits which are 35 kDa and 40 kDa in size. They show no meaningful amino acid (aa) similarity (4). The mature 35 kDa subunit (p35; also IL-12A) is an alpha -helix, 197 aa in length, that contains seven cysteines and two potential N-linked glycosylation sites (4). Mature Rhesus monkey p35 is 94%, 98%, 97%, 57%, 81%, and 82% aa identical to human, mangabey, baboon, mouse, equine and porcine mature p35, respectively. The mature 40 kDa subunit (p40; alsoIL-12B) is 306 aa in length and contains one intrachain disulfide bond plus three potential N-linked glycosylation sites. Mature Rhesus p40 is 96%, 97%, 99%, 67%, 85%, and 80% aa identical to human, mangabey, baboon, mouse, equine, and porcine p40, respectively. While p35 is reminescent of a hematopoietin ligand, p40 strongly resembles the N-terminus of a hematopoietin receptor, exhibiting a WSXWS motif, an immunoglobulin-like domain, and one fibronectin type III domain (5). This has led to the suggestion that IL-12 may be a cytokine-receptor analog to the IL-6/soluble IL-6R complex (6 - 8). Notably, while p40 may circulate as a monomer or homodimer, p35 is never found by itself. Cells known to produce IL-12 include macrophages and dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. Functionally, IL-12 has been shown to both enhance cytotoxic activity and induce interferon-gamma (IFN-gamma ) production in NK cells and T cells. In response to infection, IL-27 is released initially, promoting a Th0 to Th0/1 transition state. IL-12 follows next, generating Th1 effector cells. With IL-18, IL-12 creates Th1 memory cells out of effector cells (1 - 3, 6, 7).

Item	Value
References	Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521. Langrish, C.L. et al. (2004) Immunol. Rev. 202:96. Kreymborg, K. et al. (2005) Expert Opin. Ther. Targets 9:1123. Villinger, F. et al. (1995) J. Immunol. 155:3946. GenBank Accession # P48095. Brombacher, F. et al. (2003) Trends Immunol. 24:207. Trinchieri, G. (2003) Nat. Rev. Immunol. 3:133. Gearing, D.P. and D. Cosman (1991) Cell 66:9.
Long Name	Interleukin 12
Entrez Gene IDs	3592 (Human); 16159 (Mouse); 84405 (Rat); 397053 (Porcine); 403977 (Canine); 102115316 (Cynomolgus Monkey); 493741 (Feline); 703205 (Rhesus Macaque)
Alternate Names	CLMF p35, CLMF1, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12, IL-12 subunit p35, IL-12, subunit p35, IL12, IL12A, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, p35

Citations for Recombinant Rhesus Macaque IL-12 Protein (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rhesus Macaque IL-12 Protein include:

Species: Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Bioassay

S Watanabe et al. (2020), Protective Immune Responses Elicited by Deglycosylated Live-Attenuated Simian Immunodeficiency Virus Vaccine Are Associated with IL-15 Effector Functions J. Immunol., 0(0).
PMID: 32747501
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Bioassay

Wonderlich E et al. (2015), Macrophages and Myeloid Dendritic Cells Lose T Cell-Stimulating Function in Simian Immunodeficiency Virus Infection Associated with Diminished IL-12 and IFN-alpha Production. J Immunol, 195(7):3284-92.
PMID: 26297760
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Bioassay

Li H et al. (2014), Hypercytotoxicity and rapid loss of NKp44+ innate lymphoid cells during acute SIV infection. PLoS Pathog, 10(12):e1004551.
PMID: 25503264

Recombinant Rhesus Macaque IL-12 Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Rhesus Macaque IL-12 Protein

Formulation, Preparation and Storage

Carrier Free

3216-RL

Carrier

3216-RL/CF

Carrier Free

Reconstitution Calculator

Background: IL-12

Citations for Recombinant Rhesus Macaque IL-12 Protein (3)

Product Documents for Recombinant Rhesus Macaque IL-12 Protein

Product Datasheets

COA

Certificate of Analysis

SDS

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