Recombinant Rat Thrombopoietin Protein

Name: Recombinant Rat Thrombopoietin Protein
Brand: R&D Systems
SKU: 7864-TP

Catalog # 7864-TP | R&D Systems, Inc. a Bio-Techne Brand

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Key Product Details

Source:	NS0
Accession #:	P49745
Applications:	Bioactivity

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Product Specifications for Recombinant Rat Thrombopoietin Protein

Source	Mouse myeloma cell line, NS0-derived rat Thrombopoietin/Tpo protein Ser22-Ser326
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Starts at Ser22
Predicted Molecular Mass	32.2 kDa
SDS-PAGE	32.2 kDa
Activity	Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED₅₀ for this effect is 0.05-0.3 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

7864-TP

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7864-TP/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thrombopoietin/Tpo

Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c‑mpl. Defects in the Tpo‑Tpo R signaling pathway are associated with a variety of platelet disorders (1‑3). Mature rat Tpo shares 68% and 81% aa sequence homology with human and mouse Tpo, respectively (4). It is an 80‑85 kDa protein that consists of an N‑terminal domain with homology to Erythropoietin (Epo) and a C‑terminal domain that contains multiple N‑linked and O‑linked glycosylation sites. Tpo promotes the differentiation, proliferation, and maturation of megakaryocytes (MK) and their progenitors (5‑7). Several other cytokines can also promote these functions but only in cooperation with Tpo (8, 9). Notably, IL‑3 independently induces MK development, although its effects are restricted to early in the MK lineage (8, 9). Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation (10‑13). These actions, in combination with direct effects on cardiomyocytes, can aid in the recovery of heart function following myocardial infarction (14). Tpo is cleaved by platelet‑derived thrombin following Arg191 within the C‑terminal domain and subsequently at other sites upon extended digestion (15). The C‑terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation (6). Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia‑sensitized neurons and inhibits neuronal differentiation by blocking NGF‑induced signaling (16, 17).

Item	Value
References	de Graaf, C.A. and D. Metcalf (2011) Cell Cycle 10:1582. Kaushansky, K. (2005) J. Clin. Invest. 115:3339. Li, J. et al. (1999) Br. J. Haematol. 106:345. Ogami, K. et al. (1995) Gene 158:309. Bartley, T.D. et al. (1994) Cell 77:1117. de Sauvage, F.J. et al. (1994) Nature 369:533. Kaushansky, K. et al. (1994) Nature 369:568. Kaushansky, K. et al. (1995) Proc. Natl. Acad. Sci. USA 92:3234. Broudy, V.C. et al. (1995) Blood 85:1719. Lok, S.I. et al. (1994) Nature 369:565. Chen, J. et al. (1995) Blood 86:4054. Oda, A. et al. (1996) Blood 87:4664. Van Os, E. et al. (2003) Br. J. Haematol. 121:482. Lupia, E. et al. (2012) Mediators Inflamm. 2012:390892. Kato, T. et al. (1997) Proc. Natl. Acad. Sci. 94:4669. Ehrenreich, H. et al. (2005) Proc. Natl. Acad. Sci. USA 102:862. Samoylenko, A. et al. (2008) Cell. Signal. 20:154.
Entrez Gene IDs	7066 (Human); 21832 (Mouse); 81811 (Rat)
Alternate Names	MGDF, MGDFC-mpl ligand, MK-CSF, MKCSF, ML, MPL ligand, MPLLG, MPLLGMGC163194, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, THCYT1, THPO, TPOMKCSF, Thrombopoietin, Tpo, megakaryocyte stimulating factor, thrombopoietin nirs variant 1

Citations for Recombinant Rat Thrombopoietin Protein

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Recombinant Rat Thrombopoietin Protein

Catalog # 7864-TP | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Rat Thrombopoietin Protein

Formulation, Preparation and Storage

Carrier Free

7864-TP

Carrier

7864-TP/CF

Carrier Free

Reconstitution Calculator

Background: Thrombopoietin/Tpo

Citations for Recombinant Rat Thrombopoietin Protein

Product Documents for Recombinant Rat Thrombopoietin Protein

Product Datasheets

COA

Certificate of Analysis

SDS

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