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Recombinant Rat Notch-1 Fc Chimera Protein, CF

Catalog # 1057-TK | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
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Key Product Details



Accession #

Structure / Form

Disulfide-linked homodimer




Binding Activity

Product Specifications


Mouse myeloma cell line, NS0-derived rat Notch-1 protein
Rat Notch-1
Arg20 - Glu488 (Ala208Thr & Asp334Glu)
Accession #Q07008.2
(Pro100 - Lys330)
N-terminus C-terminus


>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis


Predicted Molecular Mass

76 kDa (monomer)


117 kDa, reducing conditions


Measured by its binding ability in a functional ELISA.
Immobilized rrNotch-1/Fc Chimera can bind rrJagged-1/Fc Chimera with an apparent KD<5 nM.

Formulation, Preparation and Storage

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Notch-1

Rat Notch-1 is a 300 kDa, type I transmembrane glycoprotein involved in a number of early-event developmental processes (1). In both vertebrates and invertebrates, Notch signaling is important for specifying cell fates and for defining boundaries between different cell types. The molecule is synthesized as a 2531 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1705 aa extracellular region, a 23 aa transmembrane (TM) segment and a 785 aa cytoplasmic domain (2). The large Notch-1 extracellular domain has 36 EGF-like repeats followed by three notch/Lin-12 repeats. Of the 36 EGF-like repeats, the 11th and 12th EGF-like repeats have been shown to be both necessary and sufficient for binding the ligands Delta and Serrate, in Drosophila (3). The Notch-1 cytoplasmic domain contains six ankyrin repeats, a glutamine-rich domain and a PEST sequence. The Notch-1 receptor undergoes post-translational proteolytic cleavage by a furin-like enzyme to form a heterodimer of the 1635 aa ligand binding extracellular region and the 877 aa transmembrane protein (4). Upon ligand binding, additional sequential proteolysis by TNF-converting enzyme and the Presenilin-dependent gamma-secretase results in the release of the Notch intracellular domain (NCID) which translocates into the nucleus where it functions as a transcription activator to initiate transcription of Notch-responsive genes (5). An alternative Notch signaling pathway that is mediated by the full-length form of Notch that has not been cleaved by the furin-like enzyme has also been reported (6). The rat Notch-1 extracellular domain shows 86% and 97% aa identity to human and mouse Notch-1 extracellular domains respectively. It also exhibits 56% and 50% aa identity with rat Notch-2 and Notch-3 extracellular domains, respectively.


  1. Weinmaster, G. (2000) Curr. Opin. Genet. Dev. 10:363.
  2. Weinmaster, G. et al. (1991) Development 113:199.
  3. Rebay, I. et al. (1991) Cell 67:687.
  4. Rogeat, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:8108.
  5. Mumm, J.S. and R. Kopan (2000) Dev. Biol. 228:151.
  6. Bush, G. et al. (2001) Dev. Biol. 229:494.

Alternate Names

Notch1, TAN1

Entrez Gene IDs

4851 (Human); 25496 (Rat)

Gene Symbol



Product Documents for Recombinant Rat Notch-1 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Rat Notch-1 Fc Chimera Protein, CF

For research use only

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