Recombinant Rat Notch-1 Fc Chimera Protein, CF

Name: Recombinant Rat Notch-1 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1057-TK

Catalog # 1057-TK

Citations (4)

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Key Product Details

Source:	NS0
Accession #:	Q07008.2
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

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Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat Notch-1 protein

Rat Notch-1 Arg20 - Glu488 (Ala208Thr & Asp334Glu) Accession #Q07008.2	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Arg20

Predicted Molecular Mass

76 kDa (monomer)

SDS-PAGE

117 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. Immobilized rrNotch-1/Fc Chimera can bind rrJagged-1/Fc Chimera with an apparent K_D<5 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1057-TK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Notch-1

Rat Notch-1 is a 300 kDa, type I transmembrane glycoprotein involved in a number of early-event developmental processes (1). In both vertebrates and invertebrates, Notch signaling is important for specifying cell fates and for defining boundaries between different cell types. The molecule is synthesized as a 2531 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1705 aa extracellular region, a 23 aa transmembrane (TM) segment and a 785 aa cytoplasmic domain (2). The large Notch-1 extracellular domain has 36 EGF-like repeats followed by three notch/Lin-12 repeats. Of the 36 EGF-like repeats, the 11^th and 12^th EGF-like repeats have been shown to be both necessary and sufficient for binding the ligands Delta and Serrate, in Drosophila (3). The Notch-1 cytoplasmic domain contains six ankyrin repeats, a glutamine-rich domain and a PEST sequence. The Notch-1 receptor undergoes post-translational proteolytic cleavage by a furin-like enzyme to form a heterodimer of the 1635 aa ligand binding extracellular region and the 877 aa transmembrane protein (4). Upon ligand binding, additional sequential proteolysis by TNF-converting enzyme and the Presenilin-dependent gamma -secretase results in the release of the Notch intracellular domain (NCID) which translocates into the nucleus where it functions as a transcription activator to initiate transcription of Notch-responsive genes (5). An alternative Notch signaling pathway that is mediated by the full-length form of Notch that has not been cleaved by the furin-like enzyme has also been reported (6). The rat Notch-1 extracellular domain shows 86% and 97% aa identity to human and mouse Notch-1 extracellular domains respectively. It also exhibits 56% and 50% aa identity with rat Notch-2 and Notch-3 extracellular domains, respectively.

Item	Value
References	Weinmaster, G. (2000) Curr. Opin. Genet. Dev. 10:363. Weinmaster, G. et al. (1991) Development 113:199. Rebay, I. et al. (1991) Cell 67:687. Rogeat, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:8108. Mumm, J.S. and R. Kopan (2000) Dev. Biol. 228:151. Bush, G. et al. (2001) Dev. Biol. 229:494.
Entrez Gene IDs	4851 (Human); 25496 (Rat)
Alternate Names	EC 2.1.2.11, EC 3.4.21.68, Notch (Drosophila) homolog 1 (translocation-associated), Notch homolog 1, translocation-associated, Notch-1, Notch1, TAN1, TAN1neurogenic locus notch homolog protein 1, Translocation-associated notch protein TAN-1, hN1, notch 1Notch homolog 1, translocation-associated (Drosophila)

Citations for Recombinant Rat Notch-1 Fc Chimera Protein, CF (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rat Notch-1 Fc Chimera Protein, CF include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

D Antfolk et al. (2017), Selective regulation of Notch ligands during angiogenesis is mediated by vimentin Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 28533359
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), Delta/Notch-Like EGF-Related Receptor (DNER) Is Not a Notch Ligand PLoS ONE, 11(9):e0161157.
PMID: 27622512
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Sneddon et al. (2012), Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme. Nature, 491(7426):765-8.
PMID: 23041930
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Bheeshmachar G et al. (2006), Evidence for a role for notch signaling in the cytokine-dependent survival of activated T cells. J. Immunol., 177(8):5041-50.
PMID: 17015687

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