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Recombinant Rat IL-23 Protein

Bio-Techne includes R&D Systems | Catalog # 3136-RL

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Carrier Free
3136-RL-010/CF
3136-RL-01M/CF

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With Carrier
3136-RL-010

Key Product Details

Source

Sf 21 (baculovirus)

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived rat IL-23 protein
Rat IL-23 p40
(Met23 - Ser335)
Accession # NP_072133
IGSGSSRGGSGSGGSGGGGSK Rat IL-23 p19
(Leu20 - Ala196)
Accession # NP_569094
8-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met23

Predicted Molecular Mass

58.3 kDa

SDS-PAGE

66 kDa, reducing conditions

Activity

Measured by its ability to induce IL-17 secretion by mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
The ED50 for this effect is 0.1-0.5 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 3136-RL
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 3136-RL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-23

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1 - 5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. The rat p19 cDNA encodes a 196 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. The mature rat p19 protein shares 88%, 78%, 76%, 75%, 71%, and 70% aa sequence identity with mouse, human, canine, equine, guinea pig, and bovine, respectively. Activated macrophages and dendritic cells express p19 and p40 concurrently to produce IL-23 (1, 4). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12R beta1) and the IL-23-specific receptor subunit (IL-23R) (3). IL-23 and IL-12 have overlapping but distinct biological activities. IL-12 drives development of Th1 cells and induces production of IFN-gamma by NK cells; IL-23 induces proliferation of Th17 cells and CD4+ memory T cells distinct from Th1 which produce IL-17, a potent proinflammatory cytokine (2). IL-23 also drives IL-17 production by NK cells and neutrophils (6). While both IL-12 and IL-23 pathways respond to infectious agents, the IL-23 - IL-17 immune pathway induces the earliest recruitment of neutrophils to the site of infection while the more classic host defense and cytotoxic response is stimulated by IL-12 (5). Dysregulation of the IL-23 - IL-17 immune pathway has a key role in organ-specific autoimmune inflammatory tissue destruction (2, 7).

References

  1. Oppmann, B. et al. (2000) Immunity 13:715.
  2. McKenzie, B.S. et al. (2006) Trends Immunol. 27:17.
  3. Parham, C. et al. (2002) J. Immunol. 168:5699.
  4. Cua, D.J. et al. (2003) Nature 421:744.
  5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
  6. Langrish, C.L. et al. (2004) Immunol. Rev. 202:96.
  7. Murphy, C.A. et al. (2003) J. Exp. Med. 198:1951.

Long Name

Interleukin 23

Alternate Names

IL-23 p19/IL-12 p40, IL-23p19/IL-12p40, IL23, SGRF

Entrez Gene IDs

51561 (Human); 83430 (Mouse); 155140 (Rat); 102128555 (Cynomolgus Monkey)

Gene Symbol

IL23A

Additional IL-23 Products

Product Documents for Recombinant Rat IL-23 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Rat IL-23 Protein

For research use only

Citations for Recombinant Rat IL-23 Protein (2)

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Reconstitution Calculator

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