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Recombinant R. sanguineus Evasin-3 His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10034-EV

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10034-EV-050

Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived r. sanguineus Evasin-3 protein
Leu21-Arg86. with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu21

Predicted Molecular Mass

7.8 kDa

SDS-PAGE

8-17 kDa, reducing conditions

Activity

Measured by its ability to inhibit CXCL8-induced chemotaxis of BaF3 mouse pro-B cells transfected with human CXCR2.
The ED50 for this effect is 0.15-0.9 μg/mL.

Scientific Data Images for Recombinant R. sanguineus Evasin-3 His-tag Protein, CF

Recombinant R. sanguineus Evasin-3 His-tag Protein Bioactivity

Recombinant R. sanguineus Evasin-3 His-tag Protein Bioactivity

Recombinant R. sanguineus Evasin-3 (Catalog # 10034-EV) inhibits IL-8/CXCL8-induced chemotaxis of BaF3 mouse pro‑B cells transfected with human CCXR2. The ED50 for this effect is 0.15-0.9 μg/mL.

Formulation, Preparation and Storage

10034-EV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: Evasin-3

Evasin-3 is a highly selective chemokine-binding protein isolated from tick saliva. The cDNA of tick Evasin-3 encodes an 86 amino acid (aa) precursor, which includes a 20 aa signal peptide and a 66 aa mature protein (1). Ticks are blood sucking parasites that secrete a wide variety of immunomodulatory proteins to evade the host immune response. The saliva isolated from Ticks has shown to contain chemokine neutralization activity. These proteins have been identified as chemokine binding proteins (CHPBs) that were termed as Evasins (1,2.3). Evasin-3 belongs to a new class of chemokine binding proteins in that it shows high affinity binding to a very limited set of chemokines, including CXCL8 and CXCL1. Since it is very small molecule Evasin-3 may be therapeutically useful as novel anti-inflammatory agent.

References

  1. Frauenschuh, A. et al. (2007) J. Biol. Chem. 282:27250.
  2. Deruaz, M. et al. (2008) J. Exp. Med. 205:2019.
  3. Deruaz, M. et al. (2013) FEBS J. 280:4876.

Long Name

Evasin-3

UniProt

Additional Evasin-3 Products

Product Documents for Recombinant R. sanguineus Evasin-3 His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant R. sanguineus Evasin-3 His-tag Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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