Spodoptera frugiperda, Sf 21 (baculovirus)-derived r. sanguineus Evasin-3 protein Leu21-Arg86. with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
8-17 kDa, reducing conditions
Measured by its ability to inhibit CXCL8-induced chemotaxis of BaF3 mouse pro-B cells transfected with human CXCR2. The ED50 for this effect is 0.15-0.9 μg/mL.
Recombinant R. sanguineus Evasin-3 His-tag Protein, CF Scientific Data Examples
Recombinant R. sanguineus Evasin-3 His-tag Protein Bioactivity
Recombinant R. sanguineus Evasin-3 (Catalog # 10034-EV) inhibits IL-8/CXCL8-induced chemotaxis of BaF3 mouse pro‑B cells transfected with human CCXR2. The ED50 for this effect is 0.15-0.9 μg/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 250 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
12 months from date of receipt, ≤ -20 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, ≤ -20 °C under sterile conditions after reconstitution.
Evasin-3 is a highly selective chemokine-binding protein isolated from tick saliva. The cDNA of tick Evasin-3 encodes an 86 amino acid (aa) precursor, which includes a 20 aa signal peptide and a 66 aa mature protein (1). Ticks are blood sucking parasites that secrete a wide variety of immunomodulatory proteins to evade the host immune response. The saliva isolated from Ticks has shown to contain chemokine neutralization activity. These proteins have been identified as chemokine binding proteins (CHPBs) that were termed as Evasins (1,2.3). Evasin-3 belongs to a new class of chemokine binding proteins in that it shows high affinity binding to a very limited set of chemokines, including CXCL8 and CXCL1. Since it is very small molecule Evasin-3 may be therapeutically useful as novel anti-inflammatory agent.
Frauenschuh, A. et al. (2007) J. Biol. Chem. 282:27250.
Deruaz, M. et al. (2008) J. Exp. Med. 205:2019.
Deruaz, M. et al. (2013) FEBS J. 280:4876.
Currently there are no citations for Recombinant R. sanguineus Evasin-3 His-tag Protein, CF.
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