Recombinant P. vulgaris Chondroitinase ABC Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6877-GH

R&D Systems, part of Bio-Techne
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Key Product Details

Source

E. coli

Accession #

P59807

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Chondroitinase ABC Proteins and Enzymes »

Product Specifications

Source

E. coli-derived p. vulgaris Chondroitinase ABC protein
Ala25-Pro1021, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

113 kDa

SDS-PAGE

85-100 kDa, reducing conditions

Activity

Measured by its ability to hydrolyze chondroitin sulfate.
The specific activity is >15,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6877-GH
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Brij-35.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Chondroitinase ABC

Chondroitinase ABC from Proteus vulgaris is a depolymerizing lyase that is especially active on various forms of chondroitin sulfate and dermatan sulfate (1, 2). Chondroitin sulfate contributes a major structural component of cartilage and provides much of the cartilage’s resistance to compression (3). Chondroitin sulfate is also found in the nervous system and at the cell surface, where it plays regulatory roles in development (4, 5) and is involved in pathways related to microbial infection (6). Dermatan sulfate is primarily located in the skin, but is also found in the blood vessels, heart valves, tendons, and lungs, where it plays roles in blood coagulation and wound healing (7). Chondroitinase ABC is also about one hundred-fold less active on hyaluronan, a nonsulfated glycosaminoglycan distributed widely throughout connective, epithelial, and neural tissues (8). Chondroitinase ABC can be used to study the structures and functions of these polysaccharides. For example, in one study it was found to promote axon growth following spinal cord injury, presumably through the degradation of chondroitin sulfate in close proximity to the axons (9). Chondroitinase ABC works through beta-elimination of the 1,4-hexosaminidic bond on the polysaccharides; thereby generates unsaturated disaccharides and tetrasaccharides that exhibit strong UV absorbance at 232 nm (10).

References

  1. Oike, Y. et al. (1980) Biochem. J. 191:193.
  2. Prabhakar, V. et al. (2009), J. Biol. Chem. 284:974.
  3. Plaas, A.H.K et al. (1998), J. Biol. Chem. 273:12642.
  4. Brittis, P.A. et al. (1992), Science 255:733.
  5. Rauch, W. (1991) J. Biol. Chem. 266:14785.
  6. Fried, M. and Duffy, P.E. Science 272:1502.
  7. Trowbridge, JM and Gallo, RL (2002) Glycobiology 12:117R.
  8. Hamai, A. et al. (1997), J. Biol. Chem. 272:9123.
  9. Garcia-Alias, G. et al. (2009) Nat. Neurosci. 12:1145.
  10. Huang, W. et al. (2007) J. Mol. Biol. 328:623.

Entrez Gene IDs

2827933 (P. vulgaris)

Gene Symbol

NEWENTRY

UniProt

P59807

Additional Chondroitinase ABC Products

Product Documents for Recombinant P. vulgaris Chondroitinase ABC Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant P. vulgaris Chondroitinase ABC Protein, CF

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