Product Specifications for Recombinant Mouse YM1/Chitinase 3-like 3 Protein, CF
Mouse myeloma cell line, NS0-derived mouse YM1/Chitinase 3-like 3 protein Met1-Tyr398, with a C-terminal 6-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
43 kDa, reducing conditions
Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. When 5 x 104 cells/well are added to Recombinant Mouse YM1/Chitinase 3-like 3 coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is 0.3-1.2 μg/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: YM1/Chitinase 3-like 3
Mouse Chitinase 3-like 3 (CHI3L3), also known as ECF-L (eosinophil chemotactic factor‑lymphocyte) or Ym1, is a secreted ~45 kDa glycoprotein that is a member of the glycosyl hydrolase family 18 (chitinase-like) protein family (1-4). Mouse CHI3L3 has no ortholog in humans, but shares 80% and 90% amino acid (aa) sequence identity with rat CHI3L3 and mouse CHI3L4 (also known as Ym2), respectively (3). Mouse CHI3L3 and CHI3L4 share partial overlap in cell type and tissue mRNA expression (2, 5). CHI3L3 is primarily secreted by alveolar and peritoneal macrophages during inflammation, for example, due to nematode infection and airway hyper-responsiveness (2-10). It is considered a marker for alternatively activated macrophages, and is also expressed in bone marrow myeloid precursors, activated microglia, bone marrow-derived immature mast cells, connective tissue-type mast cells, macrophages in erythroblastic islands, neutrophil granules in bone marrow, peritoneum and spleen red pulp, and IL‑4‑stimulated B cells and dendritic cells (DC) (2-13). Statins (cholesterol-lowering drugs) can up‑regulate CHI3L3 expression in DC and promote Th2 responses (11). CHI3L3 can form crystals when it is at high concentration in the macrophage cytoplasm (2, 3, 12, 13). Reports differ as to its enzymatic and binding properties, but CHI3L3 binding of chitin, GlcN polymer or heparin/heparan sulfate may be weak, and later studies do not indicate significant CHI3L3 chemotactic or enzymatic activity (3-5, 9, 12, 13). However, it is proposed to up‑regulate the adhesion molecules LFA‑1 (integrin alpha L beta 2) and ICAM-1, thus promoting cell-cell adhesion (14). CHI3L3 has also shown activity as a co-factor with RANKL or vitamin D in stimulating osteoclast differentiation (14, 15).
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