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Recombinant Mouse VSTM5 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10338-VT

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10338-VT-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse VSTM5 protein
Mouse VSTM5
(Leu28-His146)
Accession # Q9D806
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu28

Predicted Molecular Mass

41 kDa

SDS-PAGE

51-64 kDa, under reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells.
The ED50 for his effect is 1-6 μg/mL.

Scientific Data Images for Recombinant Mouse VSTM5 Fc Chimera Protein, CF

Recombinant Mouse VSTM5 Fc Chimera Protein Bioactivity

Recombinant Mouse VSTM5 Fc Chimera Protein Bioactivity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 1-6 μg/mL.

Formulation, Preparation and Storage

10338-VT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSTM5

V-set and transmembrane domain-containing protein 5 (VSTM5) is a cell-adhesion-like molecule found in the nervous system. It belongs to the VSTM family, a group of membrane proteins with an extracellular V-set domain and a single membrane-spanning region (1). Mouse VSTM5 is synthesized as a 199 amino acid (aa) precursor that contains a 27 aa signal sequence, a 119 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 32 aa cytoplasmic tail. Within the ECD, mouse VSTM5 shares 79% and 92% aa sequence identity with human and rat VSTM5, respectively. VSTM5 regulates morphology of the neurons during development by promotion of developing dendrites and forming synapse, and it guides both the positional migration of neuron cells and their dendritic morphology (1). N-linked glycosylation at multiple sites plays important roles for the expression, targeting, and function of VSTM5 (2).

References

  1. Lee, A.R. et al. (2016) J. Neurosci. 36:10181.
  2. Lee, A.R. et al. (2017) PLoS One 12:e0181257.

Alternate Names

C11orf90

Entrez Gene IDs

387804 (Human); 69137 (Mouse); 500947 (Rat)

Gene Symbol

VSTM5

UniProt

Additional VSTM5 Products

Product Documents for Recombinant Mouse VSTM5 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse VSTM5 Fc Chimera Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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