Recombinant Mouse VSTM5 Fc Chimera Protein, CF

Name: Recombinant Mouse VSTM5 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10338-VT

Catalog # 10338-VT

Product Datasheet / COA / SDS

Order Information

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Key Product Details

Source:	NS0
Accession #:	Q9D806
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse VSTM5 Fc Chimera Protein, CF

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse VSTM5 protein

Mouse VSTM5 (Leu28-His146) Accession # Q9D806	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu28

Predicted Molecular Mass

41 kDa

SDS-PAGE

51-64 kDa, under reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED₅₀ for his effect is 1-6 μg/mL.

Recombinant Mouse VSTM5 Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Mouse VSTM5 Fc Chimera Protein Bioactivity Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED₅₀ for this effect is 1-6 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10338-VT

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VSTM5

V-set and transmembrane domain-containing protein 5 (VSTM5) is a cell-adhesion-like molecule found in the nervous system. It belongs to the VSTM family, a group of membrane proteins with an extracellular V-set domain and a single membrane-spanning region (1). Mouse VSTM5 is synthesized as a 199 amino acid (aa) precursor that contains a 27 aa signal sequence, a 119 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 32 aa cytoplasmic tail. Within the ECD, mouse VSTM5 shares 79% and 92% aa sequence identity with human and rat VSTM5, respectively. VSTM5 regulates morphology of the neurons during development by promotion of developing dendrites and forming synapse, and it guides both the positional migration of neuron cells and their dendritic morphology (1). N-linked glycosylation at multiple sites plays important roles for the expression, targeting, and function of VSTM5 (2).

Item	Value
References	Lee, A.R. et al. (2016) J. Neurosci. 36:10181. Lee, A.R. et al. (2017) PLoS One 12:e0181257.
Entrez Gene IDs	387804 (Human); 69137 (Mouse); 500947 (Rat)
Alternate Names	C11orf90, V-set and transmembrane domain containing 5, V-set and transmembrane domain-containing protein 5, VSTM5

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