Source	Mouse myeloma cell line, NS0-derived mouse Tyro3/Dtk protein Ala31-Thr418, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala31
Predicted Molecular Mass	42 kDa
SDS-PAGE	60-75 kDa, under reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Mouse Tyro3/Dtk His-tag (Catalog # 10480-DT) is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse Gas6 (Catalog # 8310-GS) binds with an ED₅₀ of 0.4-2.4 μg/mL.

Recombinant Mouse Tyro3/Dtk His-tag Protein, CF Scientific Data Examples

	Recombinant Mouse Tyro3/Dtk His-tag Protein Binding Activity When Recombinant Mouse Tyro3/Dtk His-tag Protein is immobilized at 1 μg/mL (100 μL/well), Recombinant Mouse GAS6 (Full Length) Protein (Catalog # 8310-GS) binds with an ED₅₀ of 0.4-2.4 μg/mL.
	Recombinant Mouse Tyro3/Dtk His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Mouse Tyro3/Dtk His-tag Protein (Catalog # 10480-DT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-75 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10480-DT

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tyro3/Dtk

Dtk (Sky, Tyro3, Rse, Brt), Axl (Ufo, Ark), and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. Mouse DTK is a 42kDa protein with a 30 amino acid (aa) signal sequence, 389 aa extracellular domain (ECD), and a 20 aa transmembrane segment. The ECD of mouse DTK shares 85% and 96% sequence identity with the ECD of human and rat respectively. The extracellular domain of these proteins contains two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain. In embryoid stem cells growing in the presence of leukemia inhibitory factor, DTK is abundantly expressed and this level of expression is maintained in differentiating embryoid stem cells and cystic embryoid bodies. In mid-gestational embryos, DTK RNA is expressed in many tissues including brain, eye, thymus, lung, heart, gut, liver, testis and limbs. In contrast, expression of DTK in adult mice becomes restricted to brain, portions of gastrointestinal tract, bladder, testis and ovary. Overexpression of DTK and several other receptor tyrosine kinase have been found in small cell lung cancer (4).

Item	Value
References	Nagata, K. et al. (1996) J. Biol. Chem. 22:30022. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131. Crosier, P. et al. (1994) Growth Factors 11:125. Hamilton, G. et al. (2015) Oncoscience 2:629.
Long Name	Tyrosine Protein Kinase Receptor TYRO3
Entrez Gene IDs	7301 (Human); 22174 (Mouse)
Alternate Names	BYK, Brt, Dtk, EC 2.7.10, EC 2.7.10.1, FLJ16467, Rse, Sky, TYRO3 protein tyrosine kinase, Tif, Tyro3, Tyrosine-protein kinase DTK, Tyrosine-protein kinase RSE, Tyrosine-protein kinase SKY, Tyrosine-protein kinase byk, tyrosine-protein kinase receptor TYRO3

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Recombinant Mouse Tyro3/Dtk His-tag Protein, CF

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