Product Specifications for Recombinant Mouse Tyro3/Dtk His-tag Protein, CF
Mouse myeloma cell line, NS0-derived mouse Tyro3/Dtk protein Ala31-Thr418, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
60-75 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Tyro3/Dtk His-tag (Catalog # 10480-DT) is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse Gas6 (Catalog # 8310-GS) binds with an ED50 of 0.4-2.4 μg/mL.
Scientific Data Examples for Recombinant Mouse Tyro3/Dtk His-tag Protein, CF
Recombinant Mouse Tyro3/Dtk His-tag Protein Binding Activity
When Recombinant Mouse Tyro3/Dtk His-tag Protein is immobilized at 1 μg/mL (100 μL/well), Recombinant Mouse GAS6 (Full Length) Protein (Catalog # 8310-GS) binds with an ED50 of 0.4-2.4 μg/mL.
Recombinant Mouse Tyro3/Dtk His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Mouse Tyro3/Dtk His-tag Protein (Catalog # 10480-DT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-75 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Dtk (Sky, Tyro3, Rse, Brt), Axl (Ufo, Ark), and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. Mouse DTK is a 42kDa protein with a 30 amino acid (aa) signal sequence, 389 aa extracellular domain (ECD), and a 20 aa transmembrane segment. The ECD of mouse DTK shares 85% and 96% sequence identity with the ECD of human and rat respectively. The extracellular domain of these proteins contains two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain. In embryoid stem cells growing in the presence of leukemia inhibitory factor, DTK is abundantly expressed and this level of expression is maintained in differentiating embryoid stem cells and cystic embryoid bodies. In mid-gestational embryos, DTK RNA is expressed in many tissues including brain, eye, thymus, lung, heart, gut, liver, testis and limbs. In contrast, expression of DTK in adult mice becomes restricted to brain, portions of gastrointestinal tract, bladder, testis and ovary. Overexpression of DTK and several other receptor tyrosine kinase have been found in small cell lung cancer (4).
Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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