Is the Recombinant Mouse Gas6 (Full Length), CF (R&D Systems catalog # 8310-GS) gamma-carboxylated?

Name: Recombinant Mouse Gas6 (Full Length) Protein, CF
Brand: R&D Systems
SKU: 8310-GS
Rating: 4 (1 reviews)

R&D Systems does not generally test for the presence of post translational modifications and has not confirmed if this protein is gamma-carboxylated. However, this protein is expressed in mammalian cells (NS0-cells), therefore it is possible that post translational modifications like the Gamma-carboxyglutamate will have occurred. This protein is biologically active, contains the Gla-domain, and is cultured in the presence of vitamin K, so it is expected to have a gamma-carboxylated amino terminus.

Recombinant Mouse Gas6 (Full Length) Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 8310-GS

Citations (3)

(1)

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Key Product Details

Source:	NS0
Accession #:	CAA42507
Applications:	Binding Activity

View Full Technical Details for Recombinant Mouse Gas6 (Full Length) Protein, CF

Product Specifications for Recombinant Mouse Gas6 (Full Length) Protein, CF

Source	Mouse myeloma cell line, NS0-derived mouse Gas6 protein Ala46-Pro673, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala46 & Tyr47
Predicted Molecular Mass	71 kDa
SDS-PAGE	78-94 kDa, reducing conditions
Activity	Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. Mishra, A. et al. (2012) Mol. Cancer Res. 10:703. The ED₅₀ for this effect is 20-100 ng/mL.

Scientific Data Examples for Recombinant Mouse Gas6 (Full Length) Protein, CF

	Recombinant Mouse Gas6 (Full Length) Protein Bioactivity Recombinant Mouse Gas6 (Catalog # 8310-GS) down regulates the expression of Axl in DU145 human prostate carcinoma cells. The ED₅₀ for this effect is 20-100 ng/mL.
	Recombinant Mouse Gas6 (Full Length) Protein SDS-PAGE 1 μg/lane of Recombinant Mouse Gas6 (Full Length, Catalog # 8310-GS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing R bands at 85.4 and 69.2 kDa and NR band at 64.4 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8310-GS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Gas6

Gas6 (Growth Arrest Specific 6) is a secreted 75 kDa multimodular protein that is up-regulated by a wide variety of cell types in response to growth arrest. It is expressed by endothelial cells, fibroblasts, neurons, smooth muscle cells, and platelets, and it plays a role in vascular, thrombotic, atherosclerotic, inflammatory, autoimmune, renal, and cancer pathologies (1, 2). Both Gas6 and the related Protein S contain an extensively gamma -carboxylated N-terminal Gla domain, four EGF-like repeats, and two C-terminal Laminin G-like domains. And like Protein S, Gas6 is dependent upon vitamin K for activity. Within the Gla, EGF-like, and Laminin G-like domains, mouse Gas6 shares 83%, 94%, and 41% aa sequence identity with the equivalent regions in human Gas6, rat Gas6, and mouse Protein S, respectively. Gas6 binds to and induces signaling through the receptor tyrosine kinase TAM subfamily, which includes Axl, Dtk/Tyro3, and Mer (3). Shed soluble forms of Axl and Mer retain the ability to bind Gas6 and function as decoy receptors (4, 5). Gas6 participates in tissue homeostasis by protecting cells from stress-induced apoptosis and promoting apoptotic cell phagocytosis. The affinity of the gamma -carboxylated Gla domain for phosphatidylserine contributes to the role of Gas6 in phagocytosis as well as the cellular entry of select viruses (6, 7). Gas6 can function as a pro-inflammatory molecule by promoting platelet activation (8, 9) and can also inhibit inflammatory cytokine production from monocytes, macrophages, and microglia (10). In addition, Gas6 induces the proliferation of cardiac fibroblasts, Schwann cells, vascular smooth muscle cells, and the differentiation of NK cell precursors (11-14). It also inhibits VEGF-induced angiogenesis (15) and can have either positive or negative effects on tumor cell proliferation and invasion (16, 17).

Item	Value
References	Laurance, S. et al. (2012) Adv. Nutr. 3:196. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976. Nagata, K. et al. (1996) J. Biol. Chem. 271:30022. Sather, S. et al. (2007) Blood 109:1026. Ekman, C. et al. (2010) J. Thromb. Haemost. 8:838. Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790. Morizono, K. et al. (2011) Cell Host Microbe 9:286. Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733. Robins, R.S. et al. (2012) Blood 121:692. Alciato, F. et al. (2010) J. Leukoc. Biol. 87:869. Stenhoff, J. et al. (2004) Biochem. Biophys. Res. Commun. 319:871. Li, R. et al. (1996) J. Neurosci. 16:2012. Nakano, T. et al. (1995) J. Biol. Chem. 270:5702. Caraux, A. et al. (2006) Nat. Immunol. 7:747. Gallicchio, M. et al. (2005) Blood 105:1970. Loges, S. et al. (2010) Blood 115:2264. Gustafsson, A. et al. (2009) PLoS ONE 4:e7575.
Long Name	Growth-arrest-specific Protein 6
Entrez Gene IDs	2621 (Human); 14456 (Mouse)
Alternate Names	AXLLG, AXLLGAXL stimulatory factor, AXSFAXL receptor tyrosine kinase ligand, DKFZp666G247, FLJ34709, GAS-6, Gas6, growth arrest-specific 6, growth arrest-specific protein 6

Citations for Recombinant Mouse Gas6 (Full Length) Protein, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Gas6 (Full Length) Protein, CF include:

Species: Mouse
Sample Types: In Vivo
Applications: Bioassay

L Salmi et al. (2021-01-01), Growth Arrest-Specific Gene 6 Administration Ameliorates Sepsis-Induced Organ Damage in Mice and Reduces ROS Formation In Vitro Cells, 10(3).
PMID: 33803290
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Choi et al. (2021-01-01), Corticosterone inhibits GAS6 to govern hair follicle stem-cell quiescence Nature, 0(0).
PMID: 33790465
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

GJ Zhao et al. (2017-01-01), Growth Arrest-Specific 6 Enhances the Suppressive Function of CD4(+)CD25(+) Regulatory T Cells Mainly through Axl Receptor Mediators Inflamm, 2017(0):6848430.
PMID: 28270700

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Customer Reviews for Recombinant Mouse Gas6 (Full Length) Protein, CF (1)

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Recombinant Mouse Gas6 (Full Length) Protein, CF

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Application: In vitro bioactivity in cell culture

Verified Customer | Posted 05/12/2016

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FAQs for Recombinant Mouse Gas6 (Full Length) Protein, CF

Is the Recombinant Mouse Gas6 (Full Length), CF (R&D Systems catalog # 8310-GS) gamma-carboxylated?

R&D Systems does not generally test for the presence of post translational modifications and has not confirmed if this protein is gamma-carboxylated. However, this protein is expressed in mammalian cells (NS0-cells), therefore it is possible that post translational modifications like the Gamma-carboxyglutamate will have occurred. This protein is biologically active, contains the Gla-domain, and is cultured in the presence of vitamin K, so it is expected to have a gamma-carboxylated amino terminus.

Recombinant Mouse Gas6 (Full Length) Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Mouse Gas6 (Full Length) Protein, CF

Scientific Data Examples for Recombinant Mouse Gas6 (Full Length) Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Gas6

Citations for Recombinant Mouse Gas6 (Full Length) Protein, CF (3)

Customer Reviews for Recombinant Mouse Gas6 (Full Length) Protein, CF (1)

Recombinant Mouse Gas6 (Full Length) Protein, CF

FAQs for Recombinant Mouse Gas6 (Full Length) Protein, CF

Product Documents for Recombinant Mouse Gas6 (Full Length) Protein, CF

Product Datasheets

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