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Recombinant Mouse TREML4/TLT-4 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10172-TL

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10172-TL-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse TREML4/TLT-4 protein
Mouse TREML4/TLT-4
(Ser29-Leu197)
Accession # Q3LRV9
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser29

Predicted Molecular Mass

45 kDa

SDS-PAGE

64-72 kDa, under reducing conditions

Activity

Measured by its ability to bind apoptotic EL-4 mouse lymphoblasts in a flow cytometry assay.
The ED50 for this effect is 0.45-4.5 μg/mL.

Scientific Data Images for Recombinant Mouse TREML4/TLT-4 Fc Chimera Protein, CF

Recombinant Mouse TREML4/TLT-4 Fc Chimera Protein Binding Activity

Recombinant Mouse TREML4/TLT-4 Fc Chimera Protein Binding Activity

Recombinant Mouse TREML4/TLT-4 Fc Chimera (Catalog # 10172-TL) binds to apoptotic EL-4 mouse lymphoblasts in a flow cytometry assay. The ED50 for this effect is 0.45‑4.5 μg/mL.
Recombinant Mouse TREML4/TLT-4 Fc Chimera Protein SDS-PAGE

Recombinant Mouse TREML4/TLT-4 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse TREML4/TLT-4 Fc Chimera (Catalog # 10172-TL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 64-72 kDa and 120-150 kDa, respectively.

Formulation, Preparation and Storage

10172-TL
Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: TREML4/TLT-4

TREML4 (Triggering Receptor Expressed on Myeloid cells-like 4), also known as TLT-4, is a type I transmembrane member of the TREM family and Ig superfamily (1, 2). Mature mouse TREML4 consists of a 172 amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aa transmembrane segment, and a 42 aa cytoplasmic domain (1, 2). Within the ECD, mouse TREML4 shares 51% and 76% aa sequence identity with human and rat TREM-2, respectively. TREML4 is expressed in the spleen, CD8 alpha+ dendritic cells, and macrophages, and it is capable of recognizing necrotic cells by different phagocytes within the spleen (1). TREML4 is characterized by its Ig-like extracellular domain and short cytoplasmic tail that associates with adaptor DAP12 (2). TREML4 has been shown to play a role in coronary calcification and artery disease (3), and it is also a positive regulator of TLR7 signaling in autoimmunity and antiviral responses (4).

References

  1. Hemmi, H. et al. (2009) J. Immunol. 182:1278.
  2. Hemmi, H. et al. (2012) J. Immunol. 188:1147.
  3. Sen, S.K. et al. (2014) Am. J. Hum. Genet. 95:66.
  4. Ramirez-Ortiz, Z. G. et al. (2015) Nat. Immunol. 16:495.

Long Name

Triggering Receptor Expressed on Myeloid Cells-like 4

Alternate Names

TLT4

Entrez Gene IDs

285852 (Human); 224840 (Mouse); 680862 (Rat)

Gene Symbol

TREML4

UniProt

Additional TREML4/TLT-4 Products

Product Documents for Recombinant Mouse TREML4/TLT-4 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse TREML4/TLT-4 Fc Chimera Protein, CF

For research use only

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