Recombinant Mouse Thrombospondin-1 Protein, CF

Name: Recombinant Mouse Thrombospondin-1 Protein, CF
Brand: R&D Systems
SKU: 7859-TH

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 7859-TH

Citations (9)

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Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	NP_035710
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse Thrombospondin-1 Protein, CF

Product Specifications for Recombinant Mouse Thrombospondin-1 Protein, CF

Source

Mouse myeloma cell line, NS0-derived mouse Thrombospondin-1 protein

Mouse Thrombospondin-1 (Asn19-Ser1171) Accession # NP_035710	GGGSGGGSGGGS	6-His tag
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Starts at Asn19

Predicted Molecular Mass

129 kDa

SDS-PAGE

130-155 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED₅₀ for this effect is 0.1-0.5 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7859-TH

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thrombospondin-1

Thrombospondin‑1 (TSP‑1) is a 150‑180 kDa calcium‑sensitive protein that is secreted as a disulfide‑linked homotrimer. Within the Thrombospondin family, TSP‑2 is also homotrimeric, while TSP-3, -4, and -5/COMP are pentameric. TSP‑1 regulates a wide range of cellular functions including their interactions with other cells and with the extracellular matrix (ECM). TSP-1 contains an N‑terminal Laminin G‑like globular domain, an extended central region with one vWF‑C domain, 3 TSP type 1 repeats, 3 EGF‑like domains, and 8 TSP type‑3 repeats, and a globular C‑terminal lectin domain. Distinct regions of TSP‑1 have been associated with binding to particular ECM or cellular molecules (1, 2). Mature mouse TSP‑1 shares 95% and 99% amino acid sequence identity with human and rat TSP‑1, respectively. TSP‑1 counteracts the angiogenic, hypotensive, and anti‑thrombotic effects of nitric oxide (NO) (3). It binds and neutralizes VEGF, blocks VEGF R2 signaling on vascular endothelial cells (EC), and destabilizes adhesive contacts between EC (4‑6). TSP‑1’s actions on vascular smooth muscle cells support arterial tone and blood pressure in the presence of vasodilatory stimulation (7). It is released from activated platelets and enhances platelet aggregation and adhesion (8). TSP-1 also plays an important role in wound repair and tissue fibrosis by binding latent TGF‑beta and inducing release of the active cytokine from the latency associated peptide (LAP) (9, 10). TSP‑1 dampens adaptive immune responses by inducing the differentiation of regulatory T cells and inhibiting TCR signaling (11, 12). In the nervous system, it promotes excitatory synapse formation (13, 14) and supports the integration of neural progenitor cells into the olfactory bulb (15). In addition, TSP‑1 is released by apoptotic cells and promotes macrophage-mediated debris clearance (16).

Item	Value
References	Murphy-Ullrich, J.E. and R.V. Iozzo (2012) Matrix Biol. 31:152. Laherty, C.D. et al. (1992) J. Biol. Chem. 267:3274. Roberts, D.D. et al. (2012) Matrix Biol. 31:162. Gupta, K. et al. (1999) Angiogenesis 3:147. Kaur, S. et al. (2010) J. Biol. Chem. 285:38923. Garg, P. et al. (2011) Am. J. Physiol. Lung Cell. Mol. Physiol. 301:L79. Isenberg, J.S. et al. (2009) Matrix Biol. 28:110. Isenberg, J.S. et al. (2008) Blood 111:613. Schultz-Cherry, S. et al. (1994) J. Biol. Chem. 269:26775. Sweetwyne, M.T. and J.E. Murphy-Ullrich (2012) Matrix Biol. 31:178. Grimbert, P. et al. (2006) J. Immunol. 177:3534. Kaur, S. et al. (2011) J. Biol. Chem. 286:14991. Xu, J. et al. (2010) Nat. Neurosci. 13:22. Eroglu, C. et al. (2009) Cell 139:380. Blake, S.M. et al. (2008) EMBO J. 27:3069. Moodley, Y. et al. (2003) Am. J. Pathol. 162:771.
Entrez Gene IDs	7057 (Human); 21825 (Mouse)
Alternate Names	THBS, THBS-1, THBS1, TSP-1, TSP1thrombospondin-1, TSPTSP-1, Thrombospondin-1, Thrombospondin1, thrombospondin 1, thrombospondin-1p180

Citations for Recombinant Mouse Thrombospondin-1 Protein, CF (9)

Showing 1 - 9 of 9 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Thrombospondin-1 Protein, CF include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

C He et al. (2022), mTOR inhibition prevents angiotensin II-induced aortic rupture and pseudoaneurysm but promotes dissection in Apoe-deficient mice JCI Insight, 7(3).
PMID: 35132962
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

G Jacquemin et al. (2022), Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme Elife, 11(0).
PMID: 35543624
Species: Mouse
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR

A Jeanne et al. (2021), Targeting Ovarian Carcinoma with TSP-1:CD47 Antagonist TAX2 Activates Anti-Tumor Immunity Cancers, 13(19).
PMID: 34638503
Species: Mouse
Sample Types: Reference Standard

NMP Cartee et al. (2020), Binding of omeprazole to protein targets identified by monoclonal antibodies PLoS ONE, 15(9):e0239464.
PMID: 32946534
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

JB Conart et al. (2020), Insulin inhibits inflammation-induced cone death in retinal detachment J Neuroinflammation, 17(1):358.
PMID: 33243251
Species: Mouse
Sample Types: Whole Cells
Applications: ELISA Capture

F Wang et al. (2020), Aging-associated changes in CD47 arrangement and interaction with thrombospondin-1 on red blood cells visualized by super-resolution imaging Aging Cell, 0(0):e13224.
PMID: 32866348
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Z Liu et al. (2018), Embryonic Stem Cell-Derived Peripheral Auditory Neurons Form Neuralï¿½Connections with Mouse Central Auditory Neurons Inï¿½Vitro via theï¿½?2?1 Receptor Stem Cell Reports, 0(0).
PMID: 29887365
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2016), Thrombospondin-1 signaling through CD47 inhibits cell cycle progression and induces senescence in endothelial cells Cell Death Dis, 7(9):e2368.
PMID: 27607583
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Zheng Y et al. (2015), Photodynamic therapy-mediated cancer vaccination enhances stem-like phenotype and immune escape, which can be blocked by thrombospondin-1 signaling through CD47 receptor protein. J Biol Chem, 290(14):8975-86.
PMID: 25697354

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Showing 1 - 9 of 9 citations Showing All

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Recombinant Mouse Thrombospondin-1 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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