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Recombinant Mouse Thrombopoietin R Fc Chimera Protein, CF

Catalog # 1317-TR | R&D Systems, Inc. a Bio-Techne Brand
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1317-TR-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Thrombopoietin R/Tpo R protein
Met Mouse Tpo R
(Ser18-Trp483)
Accession # CAA52031
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

79.6 kDa (monomer)

SDS-PAGE

95-110 kDa, reducing conditions

Activity

Measured by its ability to inhibit the Tpo-dependent proliferation of MO7e human megakaryocytic leukemic cells.
The ED50 for this effect is 0.1-0.4 µg/mL in the presence of 4 ng/mL of Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO).

Formulation, Preparation and Storage

1317-TR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.

Background: Thrombopoietin R/Tpo R

Thrombopoietin receptor (Tpo R), also known as myeloproliferative leukemia protein (c-mpl), is a type I membrane protein that is a member of the hematopoietin/cytokine receptor superfamily. Thrombopoietin (Tpo) is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitro and in vivo. Receptor dimerization occurs upon ligand binding. Downstream signaling cascades utilize the Ras/MAP and JAK/STAT pathways (1). In humans, variations at the 3’ end on the molecule result in the formation of 3 distinct mRNA species, a P-form, a K-form, and a potentially secreted form. The predominant form in humans is the P-form, which encodes a full length receptor. In mouse, mRNA for a full length form, a potentially secreted form, and a form lacking 8 amino acids in the extra-cytoplasmic domain has been detected (2). The human and mouse receptors share approximately 81% amino acid identity. Tpo R is expressed at low levels in various cell types including hematopoietic progenitor cells, megakaryocytes and platelets.

References

  1. Kuter, D.J. and J. Li (2001) in Cytokine Reference, p. 1953. Oppenhiem, J.J. and M. Feldmann eds. Academic Press.
  2. Kaushansky, K. and J.G. Drachman (2002) Oncogene 21:3359.

Long Name

Thrombopoietin Receptor

Alternate Names

c-Mpl, CD110, MPL, MPLV, TpoR

Entrez Gene IDs

4352 (Human); 17480 (Mouse)

Gene Symbol

MPL

UniProt

Additional Thrombopoietin R/Tpo R Products

Product Documents for Recombinant Mouse Thrombopoietin R Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Thrombopoietin R Fc Chimera Protein, CF

For research use only

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