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Recombinant Mouse SIRP beta 1a Fc Chimera Protein, CF

Catalog # 10334-SB | R&D Systems, Inc. a Bio-Techne Brand
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10334-SB-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse SIRP beta 1a protein
Mouse SIRP beta 1a
(Val28-Gly360)
Accession # Q6F5F2
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val28

Predicted Molecular Mass

64 kDa

SDS-PAGE

83-92 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Mouse SIRP beta 1a Fc Chimera (Catalog # 10334-SB) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human SP-D (Catalog # 1920-SP) binds with an ED50 of 30-240 ng/mL.

Scientific Data Images for Recombinant Mouse SIRP beta 1a Fc Chimera Protein, CF

Recombinant Mouse SIRP beta 1a Fc Chimera Protein Binding Activity

Recombinant Mouse SIRP beta 1a Fc Chimera Protein Binding Activity

When Recombinant Mouse SIRP beta 1a Fc Chimera (Catalog # 10334-SB) is immobilized at 1 µg/mL (100 µL/well), Recombinant SP-D (Catalog # 1920-SP) binds with an ED50 of 30-240 ng/mL.
Recombinant Mouse SIRP beta 1a Fc Chimera Protein SDS-PAGE

Recombinant Mouse SIRP beta 1a Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse SIRP beta 1a Fc Chimera (Catalog # 10334-SB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 83-92 kDa and 160-180 kDa, respectively.

Formulation, Preparation and Storage

10334-SB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIRP beta 1a

Signal-regulatory protein beta 1a (SIRP beta 1a) is a disulfide-linked type I membrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin (Ig) superfamily. The SIRP family are paired receptors that have similar extracellular domains but differing C-terminal domains and functions (1). Members of this family are characterized by an extracellular region containing a V-set Ig domain containing a J-like sequence and two C1-set Ig domains. Positively charged residues within the transmembrane domain mediate interactions with DAP12 proteins which contain immunoreceptor tyrosine-based activation motifs (ITAMs) (3). Proteins in the SIRP family are typically expressed in cells of monocyte, macrophage or dendritic lineages (4). Mouse SIRP beta 1a shares 57% and 59% amino acid sequence identity with human SIRP beta 1 and rat SIRP alpha, respectively. SIRP beta 1 has a relatively short cytoplasmic region and lacks the signaling motifs for association with phosphatases. However, formation of the SIRP beta 1/DAP12 complex in myeloid cells induce tyrosine phosphorylation, mitogen-activated protein kinase activation, and cellular activation (5, 6). Engagement of SIRP beta 1 by specific monoclonal antibodies promoted Fc gamma receptor-dependent or -independent phagocytosis in mouse peritoneal macrophages (7). Surfactant protein D (Sp-D) has been shown to bind SIRP alpha and SIRP beta 1 in a calcium-dependent and sugar-specific manner on a distinct binding site from CD47 (8). Although the SIRP beta 1 extracellular regions share a high degree of homology with the SIRP alpha, SIRP beta 1 has been shown not to bind CD47 (9).

References

  1. vanBeek, E.M. et al. (2005) J. Immunol. 175:7781.
  2. van den Berg, T. et al. (2008) Trends in Immunology 29:203.
  3. Liu, Y. et al. (2005) Journal of Biological Chemistry 280:36132.
  4. Matozaki, T. et al. (2009) Trends in Cell Biology 19:72.
  5. Dietrich, J. et al. (2000) J Immunol. 164:9.
  6. Brook, G. et al. (2004) J. Immunol. 173:2562.
  7. Hayashi, A. et al. (2004) J Biol Chem. 279:29450.
  8. Fournier, B. et al. (2012) J. Biol. Chem. 287:19386.
  9. Seiffert, M. et al. (2001) Blood 97:2741.

Long Name

Signal-regulatory Protein beta 1A

Alternate Names

SIRPB1A

Entrez Gene IDs

320832 (Mouse)

Gene Symbol

SIRPB1A

UniProt

Product Documents for Recombinant Mouse SIRP beta 1a Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse SIRP beta 1a Fc Chimera Protein, CF

For research use only

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