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Recombinant Mouse Siglec-G Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10103-SL

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10103-SL-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Siglec-G protein
Mouse Siglec-G
(Met19-Lys543)
Accession # Q80ZE3.1
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met19

Predicted Molecular Mass

86 kDa

SDS-PAGE

102-114 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Siglec-G Fc Chimera (Catalog # 10103-SL) is used at 5 μg/mL, biotinylated 6'-Sialylactose-PAA binds with an ED50 of 0.2-1.2 μg/mL.

Scientific Data Images for Recombinant Mouse Siglec-G Fc Chimera Protein, CF

Recombinant Mouse Siglec-G Fc Chimera Protein Binding Activity

Recombinant Mouse Siglec-G Fc Chimera Protein Binding Activity

When Recombinant Mouse Siglec-G Fc Chimera (Catalog # 10103-SL) is used at 5 μg/mL, Biotinylated 6'-Sialylactose-PAA binds with an ED50 of 0.2-1.2 μg/mL.
Recombinant Mouse Siglec-G Fc Chimera Protein SDS-PAGE

Recombinant Mouse Siglec-G Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse Siglec-G Fc Chimera (Catalog # 10103-SL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 102-114 kDa and 205- 230 kDa, respectively.

Formulation, Preparation and Storage

10103-SL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage


Background: Siglec-G

Siglecs are type I transmembrane proteins that belong to the immunoglobulin (Ig) superfamily and function as mammalian lectins (1). They are characterized by an extracellular domain consisting of various numbers of Ig domains with a conserved N-terminal V-set Ig ligand-binding domain. This binds species-specific sialic acid motifs on protein and lipid scaffolds to regulate intracellular signaling pathways (2). The cytoplasmic tail has signaling motifs, in most cases immunoreceptor tyrosine-based inhibitory motif (ITIM) (3). Siglec-G is a member of the CD33-related Siglec family in the mouse. It is the apparent ortholog of human Siglec-10 (4). It is expressed by mature B cells, but significant levels of transcript were also detected in DC, myeloid cells, and to a lesser extent, T cells (5). Mature Siglec-G consists of a 526 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 124 aa cytoplasmic domain. Within the ECD, mouse Siglec-G shares 63% and 86% aa sequence identity with the human and rat ortholog Siglec-10, respectively. Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides (6, 7). This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell (6). When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals (8, 9).

References

  1. Crocker, P.R. et al. (2007) Nat. Rev. Immunol. 7:255.
  2. Poe, J.C. and T.F. Tedder (2012) Trends Immunol. 33:413.
  3. Meyer, S.J. et al. (2018) Front. Immunol. 9:2820.
  4. Angata, T. et al. (2001) J. Biol. Chem. 276:45128.
  5. Chen, G-Y, et al. (2014) Glycobiology 24:800.
  6. Li, N. et al. (2001) J. Biol. Chem. 276:28106
  7. Rapoport, E. et al. (2003) Bioorg. Med. Chem. Lett. 13:675.
  8. Whitney, G. et al. (2001) Eur. J. Biochem. 268:6083.
  9. Kitzig, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:355.

Long Name

Sialic Acid Binding Ig-like Lectin G

Alternate Names

Siglec-10, SiglecG

Entrez Gene IDs

243958 (Mouse)

Gene Symbol

SIGLECG

UniProt

Additional Siglec-G Products

Product Documents for Recombinant Mouse Siglec-G Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Siglec-G Fc Chimera Protein, CF

For research use only

Reconstitution Calculator

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