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Recombinant Mouse Siglec-F His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10171-SF

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10171-SF-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Siglec-F protein
Asp18-Thr437, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp18

Predicted Molecular Mass

46 kDa

SDS-PAGE

59-68 kDa, under reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965.
The ED50 for this effect is 8-48 ng/mL.

Scientific Data Images for Recombinant Mouse Siglec-F His-tag Protein, CF

Recombinant Mouse Siglec-F His-tag Protein Bioactivity

Recombinant Mouse Siglec-F His-tag Protein Bioactivity

When Recombinant Mouse Siglec-F His-tag (Catalog # 10171-SF) is immobilized onto a His Tag Antibody (Catalog # MAB050R) coated plate, it supports the adhesion of human red blood cells. The ED50 for this is 8-48 ng/mL.
Recombinant Mouse Siglec-F His-tag Protein SDS-PAGE

Recombinant Mouse Siglec-F His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse Siglec‑F His-tag (Catalog # 10171-SF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 59-68 kDa.

Formulation, Preparation and Storage

10171-SF
Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-F

Siglecs (1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins (2) belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding (3), followed by varying numbers of Ig-like C2-type domains (1, 4). Eleven human Siglecs have been cloned and characterized (1, 4). They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglec 5 to 11 (4-6). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglec 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (4, 5). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system. Mouse Siglec-F cDNA encodes a 569 amino acid polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, three Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (7). The expression of Siglec-F is restricted to the cells of myelomonocytic lineage. Mouse Siglec-F is likely an ortholog of human Siglec-5. Unlike many human CD33-related Siglecs, which show similar binding to both alpha2,3- and alpha2,6-linked sialic acids, mouse Siglec-F preferentially recognize alpha2,3-linked sialic acid.

References

  1. Crocker, P.R. et al. (1998) Glycobiology 8:v.
  2. Powell, L.D. et al. (1995) J. Biol. Chem. 270:14243.
  3. May, A.R. et al. (1998) Mol. Cell 1998. 1:719.
  4. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
  5. Crocker, P.R. et al. (2001) Immunology 103:137.
  6. Angata, T. et al. (2002) J. Biol Chem. 277:24466.
  7. Angata, T. et al. (2001) J. Biol Chem. 276:45128.

Long Name

Sialic Acid Binding Ig-like Lectin F

Alternate Names

SiglecF

Entrez Gene IDs

233186 (Mouse)

Gene Symbol

SIGLECF

UniProt

Additional Siglec-F Products

Product Documents for Recombinant Mouse Siglec-F His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Siglec-F His-tag Protein, CF

For research use only

Reconstitution Calculator

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