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His Tag Antibody

R&D Systems, Inc. a Bio-Techne Brand

Recombinant Monoclonal Antibody.
Detection of His Tag antibody by Western Blot.
(2)
Detection of His Tag antibody in HEK293 Human Cell Line Transfected with His-tagged Proteins antibody by Flow Cytometry.
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MAB050R-01M
MAB050R-100
MAB050R-SP
MAB050R-500
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Key Product Details

Immunogen: His-tagged peptide
Applications: Affinity Purification, Flow Cytometry, Western Blot
Label: Unconjugated
Source: Recombinant Monoclonal Mouse IgG1 Clone # AD1.1.10R
Specificity:

Detects proteins containing accessible consecutive histidine regions.  The antibody detects His tags located at the amino- or carboxyl- terminus.

Citations for His Tag Antibody (4)

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Customer Reviews for His Tag Antibody (8)

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  • His Tag Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: Human recombinant test antibody
    Species: Mouse
    Verified Customer | Posted 12/21/2018
  • His Tag Antibody
    Name: Anonymous
    Application: Flow
    Sample Tested: B16-F1 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 08/26/2018
  • His Tag Antibody
    Name: Anonymous
    Application: Flow
    Sample Tested: B16-F10 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 04/12/2018
  • His Tag Antibody
    Name: Anonymous
    Application: Flow
    Sample Tested: B16-F1 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 03/30/2018
  • His Tag Antibody
    Name: Robert Reddy
    Application: Flow
    Sample Tested: B16-F10 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 03/15/2018
  • His Tag Antibody
    Name: Anonymous
    Application: Flow
    Sample Tested: 786-O human renal cell adenocarcinoma cell line
    Species: Mouse
    Verified Customer | Posted 03/11/2018
  • His Tag Antibody
    Name: Amit Kumar
    Application: Flow
    Sample Tested: MCF 10A human breast epithelial cell line
    Species: Human
    Verified Customer | Posted 12/18/2017
  • His Tag Antibody
    Name: Anonymous
    Application: Flow
    Sample Tested: B16-F1 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 11/27/2017
    His Tag Antibody Flow Cytometry MAB050R

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FAQs for His Tag Antibody

  • This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

  • Can the His Tag Antibody (MAB050) recognize all His-tagged proteins?

    MAB050 has been validated specifically for the detection of His-tags localized to the amino- or carboxyl-terminus of a peptide.  It is also expected to detect any series of 6 or more histidine residues as long as this epitope is conformationally accessible. For applications utilizing linearized proteins, it is likely that MAB050 will have access to any consecutive histidine regions.  If used with protein in its native conformation, there is the potential that the protein will be folded such that the antibody is not able to bind its target.

Product Documents for His Tag Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.