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His Tag Antibody

Recombinant Monoclonal Antibody.
Detection of His Tag antibody by Western Blot.
(2)
Detection of His Tag antibody in HEK293 Human Cell Line Transfected with His-tagged Proteins antibody by Flow Cytometry.
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MAB050R-100
MAB050R-SP
MAB050R-500
MAB050R-01M

Key Product Details

Immunogen: His-tagged peptide
Applications: Affinity Purification, Flow Cytometry, Western Blot
Label: Unconjugated
Source: Recombinant Monoclonal Mouse IgG1 Clone # AD1.1.10R
Specificity:

Detects proteins containing accessible consecutive histidine regions.  The antibody detects His tags located at the amino- or carboxyl- terminus.

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Citations (2)

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Customer Reviews (8)

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  • His Tag Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: Human recombinant test antibody
    Species: Mouse
    Verified Customer | Posted 12/21/2018
  • His Tag Antibody
    Name: Anonymous
    Application: Flow
    Sample Tested: B16-F1 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 08/26/2018
  • His Tag Antibody
    Name: Anonymous
    Application: Flow
    Sample Tested: B16-F10 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 04/12/2018
  • His Tag Antibody
    Name: Anonymous
    Application: Flow
    Sample Tested: B16-F1 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 03/30/2018
  • His Tag Antibody
    Name: Anonymous
    Application: Flow
    Sample Tested: B16-F10 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 03/15/2018
  • His Tag Antibody
    Name: Anonymous
    Application: Flow
    Sample Tested: 786-O human renal cell adenocarcinoma cell line
    Species: Mouse
    Verified Customer | Posted 03/11/2018
  • His Tag Antibody
    Name: Anonymous
    Application: Flow
    Sample Tested: MCF 10A human breast epithelial cell line
    Species: Human
    Verified Customer | Posted 12/18/2017
  • His Tag Antibody
    Name: Anonymous
    Application: Flow
    Sample Tested: B16-F1 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 11/27/2017
    His Tag Antibody Flow Cytometry MAB050R

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FAQs

  • This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

Product Documents

Certificate of Analysis

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