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Recombinant Mouse Siglec-3/CD33 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10102-SL

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10102-SL-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Siglec-3/CD33 protein
Mouse Siglec-3/CD33
(Gln17-Glu240)
Accession # Q63994
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln17 inferred from enymatic pyroglutamate treatment revealing Asp18

Predicted Molecular Mass

52 kDa

SDS-PAGE

55-75 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Siglec‑H Fc Chimera (Catalog # 10264-SH) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Siglec‑3 Fc Chimera (Catalog # 10102-SL) that produces 50% of the optimal binding response is 5-20 μg/mL.

Scientific Data Images for Recombinant Mouse Siglec-3/CD33 Fc Chimera Protein, CF

Recombinant Mouse Siglec-3/CD33 Fc Chimera Protein SDS-PAGE

Recombinant Mouse Siglec-3/CD33 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse Siglec-3/CD33 Fc Chimera (Catalog # 10102-SL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-75 kDa and 110-150 kDa, respectively.

Formulation, Preparation and Storage

10102-SL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-3/CD33

Siglec-3 (sialic acid binding Ig-like lectin 3), also known as myeloid cell surface antigen CD33 and GP67, is an I-type (Ig-type) lectin belonging to the Ig superfamily. Siglecs are characterized by an N-terminal Ig-like V-type domain, which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Fourteen human and nine mouse Siglecs have been characterized and are divided into 2 families: CD33 related and evolutionarily conserved (3). Mature mouse Siglec-3 consists of a 224 amino acid (aa) extracellular domain (ECD), containing one IgV and one IgC2 domain and shares 56% aa identity with human Siglec-3. Mouse Siglec-3 is expressed on myeloid precursors in the bone marrow, mostly in the mature stages of the granulocytic lineage (4). Each Siglec has a distinct preference for binding the various types of sialylated glycans found on the surface of mammalian cells and they most likely evolved to regulate host immune responses via the recognition of self-glycans (5). Unlike human Siglec-3, mouse Siglec-3 lacks a canonical cytoplasmic ITIM domain. Instead, mouse Siglec-3 possesses a charged amino acid in its transmembrane domain, which may interact with an ITAM adaptor protein (6). Additionally, mouse Siglec-3 binds sialic acids found on mucins rather than alpha2-3- or alpha2-6-linked sialic acids on lactosamine units to which human Siglec-3 binds (7, 8). These differences suggest mouse and human Siglec-3 might not be functionally identical (8). Human Siglec-3 continues to be a therapeutic target for the treatment of acute myeloid leukemia and is a high potential risk factor for Alzheimer's (9).

Long Name

Sialic Acid Binding Ig-like Lectin 3

Alternate Names

CD33, gp67, Siglec3

Entrez Gene IDs

945 (Human); 12489 (Mouse); 102117580 (Cynomolgus Monkey)

Gene Symbol

CD33

UniProt

Additional Siglec-3/CD33 Products

Product Documents for Recombinant Mouse Siglec-3/CD33 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Manufacturing Specifications

  1. Crocker, P.R. and Varki, A. (2001) Trends Immunol. 22:337.
  2. Crocker, P.R. and Varki, A. (2001) Immunology 103:137.
  3. Macauley, M.S. et al. (2014) Nat. Rev. Immunol. 14:653.
  4. Varki, A. et al. (2017) Essentials of Glycobiology. Chapter 35.
  5. Paulson, J. et al. (2012) Ann. N. Y. Acad. Sci. 1253:37.
  6. Pillai, S. et al. (2012) Ann. Rev. Immunol. 30:357.
  7. Brinkman-Van der Linden, E.C. and Varki, A. (2000) J. Bio. Chem. 275:8633.
  8. Brinkman-Van der Linden, E.C. et al. (2003) Mol. Cell. Bio. 23:4199.
  9. Malik, M. et al. (2013) J. Neurosci. 33:13320.

Product Specific Notices for Recombinant Mouse Siglec-3/CD33 Fc Chimera Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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