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Recombinant Mouse Siglec-H Fc Chimera Protein, CF

Catalog # 10264-SH | R&D Systems, Inc. a Bio-Techne Brand
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10264-SH-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Siglec-H protein
Mouse Siglec-H
(Asn19-Gly269)
Accession # NP_848821.2
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn19

Predicted Molecular Mass

55 kDa

SDS-PAGE

71-81 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Siglec‑H Fc Chimera (Catalog # 10264-SH) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Siglec‑3 Fc Chimera (Catalog # 10102-SL) that produces 50% of the optimal binding response is 5-20 μg/mL.

Scientific Data Images for Recombinant Mouse Siglec-H Fc Chimera Protein, CF

Recombinant Mouse Siglec-H Fc Chimera Protein SDS-PAGE

Recombinant Mouse Siglec-H Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse Siglec-H Fc Chimera (Catalog # 10264-SH) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie®Blue staining, showing bands at 71-81 kDa and 140-160 kDa, respectively.

Formulation, Preparation and Storage

10264-SH
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-H

Siglecs (sialic acid-binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily (1, 2). They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (3, 4). Siglec 5 to 11 are collectively referred to as CD33-related Siglecs due to their high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (4, 5). Mouse Siglec-H is an endocytic receptor expressed predominantly on a specialized subset of leukocytes, the plasmacytoid dendritic cell (pDC). Siglec-H has not been shown to bind sialic acids (6). Unlike other CD33-related siglecs, the cytoplasmic tail of Siglec-H lacks ITIM-related sequence (6, 7). The transmembrane region contains a charged lysine residue that could associate with DAP12 which reduces interferon-alpha (IFN alpha) production (6, 8).

References

  1. Crocker, P.R. et al. (1998) Glycobiology 8:v.
  2. Powell, L.D. et al. (1995) J. Biol. Chem. 270:14243.
  3. May, A.R. et al. (1998) Mol. Cell (1998) 1:719.
  4. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
  5. Crocker, P.R. et al. (2001) Immunology 103:137.
  6. Zhang, J. et al. (2006) Blood 107:3600.
  7. Blasius, A.L. and M. Colonna (2006) Trends Immunol. 27:255.
  8. Blasius, A.L. et al. 2006. Blood 107:2474.

Long Name

Sialic Acid Binding Ig-like Lectin 7

Alternate Names

SiglecH

Entrez Gene IDs

233274 (Mouse)

Gene Symbol

SIGLECH

UniProt

Product Documents for Recombinant Mouse Siglec-H Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Siglec-H Fc Chimera Protein, CF

For research use only

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