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Recombinant Mouse Siglec-15 His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10288-SL

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10288-SL-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Siglec-15 protein
Arg24-Tyr250, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg24

Predicted Molecular Mass

25 kDa

SDS-PAGE

30-45 kDa, under reducing conditions

Activity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3.
The ED50 for this effect is 0.075‑0.75 μg/mL.

Scientific Data Images for Recombinant Mouse Siglec-15 His-tag Protein, CF

Recombinant Mouse Siglec-15 His-tag Protein Bioactivity

Recombinant Mouse Siglec-15 His-tag Protein Bioactivity

Recombinant Mouse Siglec-15 His-tag (Catalog # 10288-SL) inhibits IL-2 secretion by mouse T cells in the presence of Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841). The ED50 for this effect is 0.075-0.75 µg/mL.
Recombinant Mouse Siglec-15 His-tag Protein SDS-PAGE

Recombinant Mouse Siglec-15 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse Siglec-15 His-tag (Catalog # 10288-SL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-45 kDa.

Formulation, Preparation and Storage

10288-SL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-15

Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Mature mouse Siglec-15 consists of a 239 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 59 aa cytoplasmic domain. Within the ECD, mouse Siglec-15 shares 85% and 92% aa sequence identity with human and rat Siglec-15, respectively. In human Siglec-15, alternative splicing generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 associates with the signaling molecules DAP12 and DAP10 (2-5). It is expressed on osteoclasts, macrophages, and dendritic cells (2-6) and binds to the sialyl-Tn antigen (2, 3, 6). This interaction induces the production of TGF-beta by tumor-associated macrophages (3). Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts (4-6). Siglec-15 is broadly upregulated on human cancer cells and tumor infiltrating myeloid cells (7). Siglec-15 suppresses antigen-specific T cell responses in vitro and in vivo therefore it is considered to be an immune suppressor and potential target for normalization cancer immunotherapy (7).

References

  1. Humphrey, M.B. and M.C. Nakamura (2016) Clin. Rev. Allergy Immunol. 51:48.
  2. Angata, T. et al. (2007) Glycobiology 17:838.
  3. Takamiya, R. et al. (2013) Glycobiology 23:178.
  4. Kameda, Y. et al. (2013) J. Bone Miner. Res. 28:2463.
  5. Stuible, M. et al. (2014) J. Biol. Chem. 289:6498.
  6. Ishida-Kitagawa, N. et al. (2012) J. Biol. Chem. 287:17493.
  7. Wang, J. et al. (2019) Nat. med. 25:656.

Long Name

Sialic Acid Binding Ig-like Lectin 15

Alternate Names

CD33L3, HsT1361, Siglec15

Entrez Gene IDs

284266 (Human); 620235 (Mouse); 498888 (Rat)

Gene Symbol

SIGLEC15

UniProt

Additional Siglec-15 Products

Product Documents for Recombinant Mouse Siglec-15 His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Siglec-15 His-tag Protein, CF

For research use only

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