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Recombinant Mouse Serpin A1c/alpha-1-Antitrypsin Protein, CF

Catalog # 2979-PI | R&D Systems, Inc. a Bio-Techne Brand
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2979-PI-010

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Inhibition Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Serpin A1c/alpha 1-Antitrypsin protein
Glu25-Lys413, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu25

Predicted Molecular Mass

45 kDa

SDS-PAGE

60 kDa, reducing conditions

Activity

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is <10 nM, as measured under the described conditions.

Formulation, Preparation and Storage

2979-PI
Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Serpin A1c/alpha 1-Antitrypsin

Serpin A1 is the archetypal member of the Serpin superfamily of the serine protease inhibitors (1). As one of the most abundant proteinase inhibitors in the circulation, it is synthesized in the liver and secreted into the bloodstream with the major function to protect tissues against neutrophil elastase. Severe Serpin A1 deficiency leads to several clinical complications such as pulmonary emphysema, juvenile hepatitis, cirrhosis, and hepatocellular carcinoma (2). In contrast to a single Serpin A1 gene in the human genome, there is a cluster of five Serpin A1 genes in the mouse genome with high overall sequence identity but markedly divergent residues in the reactive center loop (3, 4). The purified rmSerpin A1c inhibits trypsin and chymotrypsin, but does not inhibit neutrophil elastase.

References

  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Barbour, K.W. et al. (2002) Genomics 80:515.
  3. Forsyth, S. et al. (2003) Genomics 81:336.
  4. Puente, X.S. and Lopez-Otin, C. (2004) Genome Res. 14:609.

Alternate Names

alpha 1Antitrypsin, Spi1-3, Spi1-6, Wap3

Entrez Gene IDs

5265 (Human); 20702 (Mouse)

Gene Symbol

SERPINA1

UniProt

Product Documents for Recombinant Mouse Serpin A1c/alpha-1-Antitrypsin Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Serpin A1c/alpha-1-Antitrypsin Protein, CF

For research use only

FAQs for Recombinant Mouse Serpin A1c/alpha-1-Antitrypsin Protein, CF

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  • Q: Serpin A1 is a serine protease inhibitor. Does Mouse Serpin A1c inhibit all serine proteases?

    A: Mouse Serpin A1c inhibits trypsin and chymotrypsin, but it does not inhibit neutrophil elastase.

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