Recombinant Mouse Semaphorin 3C Fc Chimera (Truncated), CF

Name: Recombinant Mouse Semaphorin 3C Fc Chimera (Truncated), CF
Brand: R&D Systems
SKU: 1728-S3

Catalog # 1728-S3 | R&D Systems, Inc. a Bio-Techne Brand

Citations (3)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	Sf 21 (baculovirus)
Accession #:	Q62181
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse Semaphorin 3C Fc Chimera (Truncated), CF

Technical Data
Citations (3) / Reviews

Product Specifications for Recombinant Mouse Semaphorin 3C Fc Chimera (Truncated), CF

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Semaphorin 3C protein

MHHHHHH	Mouse Semaphorin 3C (Gln24-Ser741, Arg548Ala, Arg552Ala) Accession # Q62181	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus			C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Met

Predicted Molecular Mass

108.9 kDa (monomer)

SDS-PAGE

108.9 kDa (monomer)

Activity

Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED₅₀ for this effect is 0.12-1.2 μg/mL.

Scientific Data Examples for Recombinant Mouse Semaphorin 3C Fc Chimera (Truncated), CF

	Recombinant Mouse Semaphorin 3C Fc Chimera (Truncated) Bioactivity Immobilized Recombinant Mouse Semaphorin 3C Fc Chimera (Truncated) (Catalog # 1728‑S3) supports the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED₅₀ for this effect is 0.12-1.2 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1728-S3

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris, Citric Acid, NaCl and Tween® 20.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Desired Reconstitution Concentration

Background: Semaphorin 3C

Semaphorin 3C (Sema3C; previously semaE) is one of six Class 3 secreted semaphorins which share 40-50% amino acid (aa) identity. Class 3 semaphorins are potent chemorepellents that function in axon and/or vascular guidance during development, and may be upregulated in tumor progression (1, 2). The 751 amino acid (aa) mouse Sema3C is highly modular. It contains a 20 aa signal sequence, an ~500 aa N-terminal Sema domain that forms a beta -propeller structure similar to that found in integrin molecules, a cysteine knot, a furin-type cleavage site, an Ig-like domain, and a C-terminal basic domain (1-3). Covalent dimerization plus cleavage at the C-terminus are required for activity of class 3 semaphorins (4). Mouse Sema3C shares at least 95% aa identity with human, rat, cow and dog Sema3C, and 89% and 75% aa identity with chick and zebrafish Sema3C, respectively. Type 3 semaphorins transduce signals through transmembrane plexins, either directly or by binding associated neuropilin receptors (1, 2). Sema3C signaling is transduced by Plexin-D1 indirectly via neuropilin-1 or neuropilin-2 receptors (5). Sema3C is expressed in all somitic motor neurons, in lung buds and in cardiac neural crest cells during development (1, 5-8). Sema3C activates integrins in certain cells so, in addition to its repulsive activities, it sometimes acts as a chemoattractant (6, 9). In the developing nervous system, this chemoattraction appears to complement Sema3A repulsion in adjacent cell layers (1, 6, 7). Sema3C also provides an attractive force opposing Sema6A and Sema6B to guide migration of neural crest endothelial cells to the cardiac outflow tract (10). Consequently, defects in aortic arch formation occur when Sema3C or Plexin-D1 genes or Sema3C-neuropilin interactions are disrupted (5, 11, 12).

Item	Value
References	Hinck, L. (2004) Dev. Cell 7:783. Neufeld, G. et al. (2005) Front. Biosci. 10:751. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669. Adams, R. H. et al. (1997) EMBO J. 16:6077. Gitler, A. D. et al. (2004) Dev. Cell 7:107. Bagnard, D. et al. (1998) Development 125:5043. Cohen, S. et al. (2005) Eur. J. Neurosci. 21:1767. Puschel, A. W. et al. (1995) Neuron 14:941. Herman, J. G. and G. G. Meadows (2007) Int. J. Oncol. 30:1231. Toyofuku, T. et al. (2008) Dev. Biol. 321:251. Feiner, L. et al. (2001) Development 128:3061. Gu, C. et al. (2003) Dev. Cell 5:45.
Entrez Gene IDs	10512 (Human); 20348 (Mouse)
Alternate Names	(semaphorin) 3C, SEMA3C, SEMAE, SEME, Sema E, Semaphorin 3C, Semaphorin E, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, semaphorin-3C, semaphorin-E

Citations for Recombinant Mouse Semaphorin 3C Fc Chimera (Truncated), CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Semaphorin 3C Fc Chimera (Truncated), CF include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

V Mohan et al. (2019-06-10), Close Homolog of L1 Regulates Dendritic Spine Density in the Mouse Cerebral Cortex through Semaphorin 3B J. Neurosci., 2019-00(0).
PMID: 31182634
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

K Burk et al. (2017-02-22), Post-endocytic sorting of Plexin-D1 controls signal transduction and development of axonal and vascular circuits Nat Commun, 2017-08(0):14508.
PMID: 28224988
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Plein A et al. (2015-06-08), Neural crest-derived SEMA3C activates endothelial NRP1 for cardiac outflow tract septation. J Clin Invest, 2015-0125(7):2661-76.
PMID: 26053665

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Recombinant Mouse Semaphorin 3C Fc Chimera (Truncated), CF

Catalog # 1728-S3 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Mouse Semaphorin 3C Fc Chimera (Truncated), CF

Scientific Data Examples for Recombinant Mouse Semaphorin 3C Fc Chimera (Truncated), CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Semaphorin 3C

Citations for Recombinant Mouse Semaphorin 3C Fc Chimera (Truncated), CF (3)

Product Documents for Recombinant Mouse Semaphorin 3C Fc Chimera (Truncated), CF

Product Datasheets

COA

Certificate of Analysis

SDS

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