Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Recombinant Mouse Rae-1 epsilon Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1135-RA

Catalog #
Availability
Size / Price
Qty
Loading...
1135-RA-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Rae-1 epsilon protein
Mouse Rae-1 epsilon
(Leu29 - Ser229)
Accession #Q9CZQ6
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu29

Predicted Molecular Mass

49.3 kDa (monomer)

SDS-PAGE

65-75 kDa, reducing conditions

Activity

Measured by the ability of the protein to bind to immobilized rmNKG2D/Fc Chimera in a functional ELISA.

Formulation, Preparation and Storage

1135-RA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background:

Rae-1 epsilon is a member of a family of cell-surface proteins that function as ligands for mouse NKG2D. Other family members are designated Rae-1 alpha, beta, gamma, and delta. Amino acid sequence identity within this family ranges from 88 - 95%. The Rae-1 proteins are distantly related to MHC class I proteins, but they possess only the alpha1 and alpha2 Ig-like domains, and they have no capacity to bind peptide or interact with beta2-microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. The Rae-1 proteins are anchored to the membrane via a GPI-linkage. The name of this family derives from the original identification of these proteins as the product of retinoic acid early inducible transcripts. Rae-1 expression is developmentally controlled. Transcripts were observed in the brain/head region of day 10 - 14 embryos but disappeared by day 18. Rae-1 transcripts were detected in several transformed cell lines but are absent from most normal adult tissues. All Rae-1 family members bind to mouse NKG2D, an activating receptor expressed on NK cells and some T cell subsets, resulting in the activation of cytolytic activity and/or cytokine production by these effector cells. Ectopic expression of Rae-1 on mouse tumor cell lines resulted in the in vivo rejection of the tumors (1 - 6).

References

  1. Zou, Z. et al. (1996) J. Biochem (Tokyo) 119:319.
  2. Diefenbach, A. et al. (2000) Nature Immunol. 1:119.
  3. Cerwenka, A. et al. (2000) Immunity 12:721.
  4. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
  5. Diefenbach, A. et al. (2001) Nature 413:165.
  6. NKG2D and its Ligands, www.RnDSystems.com.

UniProt

Product Documents for Recombinant Mouse Rae-1 epsilon Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Rae-1 epsilon Fc Chimera Protein, CF

For research use only

Citations for Recombinant Mouse Rae-1 epsilon Fc Chimera Protein, CF (2)

Showing  1 - 2 of 2 publications Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Rae-1 epsilon Fc Chimera Protein, CF include:

There are no citations that match your criteria.

Showing  1 - 2 of 2 publications Showing All

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=