Recombinant Mouse Rae-1 epsilon Fc Chimera Protein, CF

Name: Recombinant Mouse Rae-1 epsilon Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1135-RA
Price: 405 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1135-RA

Citations (2)

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Key Product Details

Source:	NS0
Accession #:	Q9CZQ6
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

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Product Specifications for Recombinant Mouse Rae-1 epsilon Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived mouse Rae-1 epsilon protein

Mouse Rae-1 epsilon (Leu29 - Ser229) Accession #Q9CZQ6	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu29

Predicted Molecular Mass

49.3 kDa (monomer)

SDS-PAGE

65-75 kDa, reducing conditions

Activity

Measured by the ability of the protein to bind to immobilized rmNKG2D/Fc Chimera in a functional ELISA.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1135-RA

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Rae-1 epsilon

Rae-1 epsilon is a member of a family of cell-surface proteins that function as ligands for mouse NKG2D. Other family members are designated Rae-1 alpha, beta, gamma, and δ. Amino acid sequence identity within this family ranges from 88 - 95%. The Rae-1 proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. The Rae-1 proteins are anchored to the membrane via a GPI-linkage. The name of this family derives from the original identification of these proteins as the product of retinoic acid early inducible transcripts. Rae-1 expression is developmentally controlled. Transcripts were observed in the brain/head region of day 10 - 14 embryos but disappeared by day 18. Rae-1 transcripts were detected in several transformed cell lines but are absent from most normal adult tissues. All Rae-1 family members bind to mouse NKG2D, an activating receptor expressed on NK cells and some T cell subsets, resulting in the activation of cytolytic activity and/or cytokine production by these effector cells. Ectopic expression of Rae-1 on mouse tumor cell lines resulted in the in vivo rejection of the tumors (1 - 6).

Item	Value
References	Zou, Z. et al. (1996) J. Biochem (Tokyo) 119:319. Diefenbach, A. et al. (2000) Nature Immunol. 1:119. Cerwenka, A. et al. (2000) Immunity 12:721. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521. Diefenbach, A. et al. (2001) Nature 413:165. NKG2D and its Ligands, www.RnDSystems.com.
Long Name	Retinoic Acid Early Transcript 1 epsilon
Entrez Gene IDs	8480 (Human); 379043 (Mouse)
Alternate Names	Rae-1 epsilon, Rae1 epsilon

Citations for Recombinant Mouse Rae-1 epsilon Fc Chimera Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Rae-1 epsilon Fc Chimera Protein, CF include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Han et al. (2020-01-01), Early growth response genes 2 and 3 induced by AP-1 and NF-&kappaB modulate TGF-&beta1 transcription in NK1.1- CD4+ NKG2D+ T cells Cell Signal, 76(0):109800.
PMID: 33011290
Species: Mouse
Sample Types: Serum
Applications: Bioassay

Lin Z et al. (2014-01-01), CD4(+) NKG2D(+) T cells induce NKG2D down-regulation in natural killer cells in CD86-RAE-1epsilon transgenic mice. Immunology, 141(3):401-15.
PMID: 24708417

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Recombinant Mouse Rae-1 epsilon Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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