Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein

Name: Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein
Brand: R&D Systems
SKU: 7150-RS
Rating: 5 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 7150-RS

Citations (8)

(3)

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Key Product Details

Source:	CHO
Accession #:	NP_619624
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein

Source	Chinese Hamster Ovary cell line, CHO-derived mouse R-Spondin 1 protein Met1-Gln265
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser21
Predicted Molecular Mass	27 kDa
SDS-PAGE	40-43 kDa, reducing conditions
Activity	Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED₅₀ is 8-40 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).

Scientific Data Examples for Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein

	Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein Bioactivity Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED₅₀ is 8-40 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (1324-WN).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

7150-RS

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7150-RS/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: R-Spondin 1

R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members (1, 2). All R-Spondins regulate Wnt/ beta-Catenin signaling but have distinct expression patterns (1-3). R-Spondin 1 competes with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization (4). However, reports are mixed on whether R-Spondin 1 binds LRP-6 directly (4-6). R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 7). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (7, 8). Mutations in R-Spondin 1 are also linked with palmoplantar keratoderma, abnormal thickening of the skin on the palms of the hands and soles of the feet (7, 8). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (9). Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (9, 10). Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids (11).

Structurally similar to other R-Spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin-like domains are needed for beta-catenin stabilization (2, 12). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (13). Mouse R‑Spondin 1 shares 98%, 94%, 94%, 93%, 92% and 88% aa identity with rat, human, horse, cow, goat and dog RSPO-1, respectively, within aa 21‑209.

Item	Value
References	Chen, J.-Z. et al. (2002) Mol. Biol. Rep. 29:287. Kim, K.-A. et al. (2006) Cell Cycle 5:23. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51. Parma, P. et al. (2006) Nat. Genet. 38:1304. Kim, K.-A. et al. (2005) Science 309:1256. Zhao, J. et al. (2007) Gastroenterology 132:1331. Drost and Clevers. (2018) Nature Reviews Cancer 18:407. Kazanskaya, O. et al. (2004) Dev. Cell 7:525. Tomaselli, S. et al. (2008) Hum. Mutat. 29:220.
Long Name	Roof Plate-specific Spondin 1
Entrez Gene IDs	284654 (Human); 192199 (Mouse); 102122369 (Cynomolgus Monkey)
Alternate Names	CRISTIN3, Cristin 3, FLJ40906Roof plate-specific spondin-1, HRspo1, R-Spondin 1, R-spondin homolog (Xenopus laevis), R-spondin-1, R-spondin1, RSPO, RSPO1, RSPONDIN, RSpondin 1, roof plate-specific spondin

Citations for Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein (8)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein include:

Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Z Fu et al. (2021), Mitochondrial transcription factor A in RORgammat+ lymphocytes regulate small intestine homeostasis and metabolism Nature Communications, 12(1):4462.
PMID: 34294718
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

MW Dougherty et al. (2020), Gut microbiota maturation during early human life induces enterocyte proliferation via microbial metabolites BMC Microbiol., 20(1):205.
PMID: 32652929
Species: Mouse
Sample Types: Whole Tissue
Applications: Cell Culture

K Dunbar et al. (2020), Aspirin Rescues Wnt-Driven Stem-like Phenotype in Human Intestinal Organoids and Increases the Wnt Antagonist Dickkopf-1 Cell Mol Gastroenterol Hepatol, 0(0).
PMID: 32971322
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Y Cai et al. (2019), Indispensable role of the Ubiquitin-fold modifier 1-specific E3 ligase in maintaining intestinal homeostasis and controlling gut inflammation Cell Discov, 5(0):7.
PMID: 30701081
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

G Greicius et al. (2018), PDGFR? + pericryptal stromal cells are the critical source of Wnts and RSPO3 for murine intestinal stem cells in vivo Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 29559533
Species: Mouse
Sample Types: Intestinal organoids
Applications: Bioassay

Y Eriguchi et al. (2018), Essential role of IFN-? in T cell-associated intestinal inflammation JCI Insight, 3(18).
PMID: 30232288
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

X Yin et al. (2016), RSPOs facilitated HSC activation and promoted hepatic fibrogenesis Oncotarget, 7(39):63767-63778.
PMID: 27572318
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Marie-Isabelle Garcia (2016), Trop2 marks transient gastric fetal epithelium and adult regenerating cells after epithelial damage Development, 143(9):1452-63.
PMID: 26989172

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Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein

Name: Anonymous

Application: CellProlif

Verified Customer | Posted 06/16/2020
Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein

Name: FAYROUZ BAZINA

Application: CellProlif

Verified Customer | Posted 12/22/2017
Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein

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Application: Cell migration/motility

Verified Customer | Posted 05/18/2016

Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

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Product Specifications for Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein

Scientific Data Examples for Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein

Formulation, Preparation and Storage

Carrier Free

7150-RS

Carrier

7150-RS/CF

Carrier Free

Reconstitution Calculator

Background: R-Spondin 1

Citations for Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein (8)

Customer Reviews for Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein (3)

Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein

Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein

Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein

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