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Recombinant Mouse Prolactin Protein

Bio-Techne includes R&D Systems | Catalog # 1445-PL

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Carrier Free
1445-PL-050/CF

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With Carrier
1445-PL-050

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived mouse Prolactin protein
Leu32-Cys228, with an N-terminal Met

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

22.6 kDa

Activity

Measured in a cell proliferation assay using Nb2‑11 rat lymphoma cells. Gout, P.W. et al. (1980) Cancer Res. 40:2433.
The ED50 for this effect is 0.25-1 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1445-PL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1445-PL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Prolactin

Prolactin (gene name PRL) is a secreted neuroendocrine pituitary hormone that acts primarily on the mammary gland to promote lactation, but has pleiotropic effects in both males and females (1-3). Mouse Prolactin is predominantly found as 23 kDa non-glycosylated monomers. Mouse prolactin shares 60% and 85% amino acid sequence identity with human and rat prolactin, respectively; however, activation of the human prolactin receptor by rat prolactin has been demonstrated (3). Prolactin is synthesized mainly by the anterior pituitary in all mammals, where secretion is under tonic inhibition by hypothalamic dopamine (2, 3). Secretion can be stimulated by suckling and diurnally by estradiol in rodents during pro-estrus and mating (2). In humans prolactin is also produced peripherally, while in rodents, the only known non-pituitary source is the placenta (1-3). Mouse serum Prolactin declines until mid-pregnancy, but increases in late pregnancy (1). Post-translational modifications such as polymerization, phosphorylation, and proteolytic cleavage can alter the activities of prolactin (3). Cleavage by matrix metalloproteinases or Cathepsin D can produce N-terminal 16 kDa antiangiogenic fragments also called vasoinhibins (4, 5). Thrombin can produce C-terminal 16 kDa fragments that are not antiangiogenic (3). The prolactin receptor (gene name PRLR) is a transmembrane type I glycoprotein that belongs to the cytokine hematopoietic receptor family. Expression of the prolactin receptor is widespread (2, 3). Each prolactin molecule is thought to bind two receptor molecules (6).

References

  1. Linzer, D.I.H. et al. (1985) J. Biol. Chem. 260:9574.
  2. Grattan, D.R. and I.C. Kokay (2008) J. Neuroendocrinol. 20:752.
  3. Ben-Jonathan, N. et al. (2008) Endocr. Rev. 29:1.
  4. Piwnica, D. et al. (2006) Mol. Endocrinol. 20:3263.
  5. Macotela, Y. et al. (2006) J. Cell Sci. 119:1790.
  6. Broutin, I. et al. (2010) J. Biol. Chem. 285:8422.

Alternate Names

PRL

Entrez Gene IDs

5617 (Human); 19109 (Mouse)

Gene Symbol

PRL

UniProt

Additional Prolactin Products

Product Documents for Recombinant Mouse Prolactin Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Prolactin Protein

For research use only

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Reconstitution Calculator

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