Recombinant Mouse Pleiotrophin/PTN Protein, CF

Name: Recombinant Mouse Pleiotrophin/PTN Protein, CF
Brand: R&D Systems
SKU: 6580-PL

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 6580-PL

Citations (3)

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Source:	Sf 21 (baculovirus)
Accession #:	P63089
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse Pleiotrophin/PTN Protein, CF

Source	Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Pleiotrophin/PTN protein Met1-Asp168
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Gly33
Predicted Molecular Mass	15.3 kDa
SDS-PAGE	19 kDa, reducing conditions
Activity	Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cerebral cortical neurons. Muramatsu, H. and T. Muramatsu (1991) Biochem. Biophys. Res. Commu. 177:652. Optimal neurite outgrowth was observed when neurons were plated on 96 well culture plates that had been pre-coated with 100 µL/well of recombinant mouse Pleiotrophin at 3‑8 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6580-PL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Pleiotrophin/PTN

Pleiotrophin (PTN), also called heparin-binding growth-associated molecule (HB-GAM), heparin-binding neurotrophic factor (HBNF), heparin-affinity regulatory peptide (HARP), or osteoblast-specific factor (OSF-1), is an 18 kDa secreted, strongly heparin-binding, developmentally regulated cytokine (1 ‑ 3). PTN and midkine share 50% amino acid (aa) sequence identity, share some functions, and constitute a family (1 ‑ 3). The mouse PTN cDNA encodes 168 aa, including a 32 aa signal sequence and two thrombospondin type 1 (TSP1) beta sheet domains separated by a linker and flanked by lysine-rich N- and C-terminal sequences (4). The second TSP1 domain (aa 97 ‑ 129) contains the highest affinity binding site for heparin (4, 5). A 15 kDa form which lacks the C-terminus is mitogenic for glioblastoma cells, while full-length PTN is not (6). PTN is a highly conserved protein; human, mouse, rat, canine, porcine, equine and bovine PTN share 98% aa sequence identity or greater. During development, PTN is involved in development of brain, bone, and organs undergoing branching morphogenesis (3). In the adult, it is induced by PDGF and upregulated in many cancers, hematopoietic stem cells and tissues undergoing remodeling (7 ‑ 10). Cell surface receptors for PTN include Syndecan-3 (which mediates neurite outgrowth) and the receptor tyrosine phosphatase PTPRB, also called RPTP beta / zeta (3, 11 ‑ 13). Heparin binding is necessary for engaging these receptors (7, 8). PTN causes PTPRB dimerization and inactivates its phosphatase activity, which allows increased tyrosine phosphorylation of its substrates (12 ‑ 14). One such substrate is the WNT pathway molecule beta -catenin, allowing crosstalk of PTN with WNTs (12). PTN activation of the receptor ALK (anaplastic lymphoma kinase) is indirect through PTPRB, and mediates mitogenic, transforming and angiogenic activities of PTN (2, 5, 6, 13). Increased expression of PTN is correlated with neuronal development or stresses such as brain ischemia and Parkinson’s disease (2, 3, 7, 8). Both PTN and midkine have demonstrated bactericidal activity, but only in the absence of heparin (15).

Item	Value
References	Naito, A. et al. (1992) Biochem. Biophys. Res. Commun. 183:701. Perez-Pinera, P. et al. (2008) Curr. Opin. Hematol. 15:210. Weng, T. and L. Liu (2010) Respir. Res. 11:80. Raulo, E. et al. (2005) J. Biol. Chem. 280:41576. Hamma-Kourbali, Y. et al. (2008) J. Cell. Physiol. 214:250. Lu, K.V. et al. (2005) J. Biol. Chem. 280:16953. Yeh, H-J. et al. (1998) J. Neurosci. 18:3699. Marchionini, D.M. et al. (2007) Brain Res. 1147:77. Chang, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:10888. Himburg, H.A. et al. (2010) Nat. Med. 16:475. Kinnunen, T. et al. (1996) J. Biol. Chem. 271:2243. Meng, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2603. Perez-Pinera, P. et al. (2007) J. Biol. Chem. 282:28683. Fukada, M. et al. (2006) FEBS Lett. 580:4051. Svensson, S.L. et al. (2010) J. Biol. Chem. 285:16105.
Entrez Gene IDs	5764 (Human)
Alternate Names	HARP, HB-GAM, HBBM, HBGF8, HBNF, HBNF-1, HBNF1, HNGF-8, Heparin-binding brain mitogen, NEGF1HBGF-8, OSF-1, Osteoblast-specific factor 1, PTN, Pleiotrophin, neurite growth-promoting factor 1, neurite growth-promoting factor1)

Citations for Recombinant Mouse Pleiotrophin/PTN Protein, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Pleiotrophin/PTN Protein, CF include:

Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

HA Himburg et al. (2019), Chronic myelogenous leukemia stem cells require cell-autonomous pleiotrophin signaling J. Clin. Invest., 0(0).
PMID: 31613796
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

AM Nikolakopo et al. (2019), Pericyte loss leads to circulatory failure and pleiotrophin depletion causing neuron loss Nat. Neurosci., 22(7):1089-1098.
PMID: 31235908
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Gui T et al. (2013), The loss of Trps1 suppresses ureteric bud branching because of the activation of TGF-beta signaling. Dev Biol, 377(2):415-27.
PMID: 23537899

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Recombinant Mouse Pleiotrophin/PTN Protein, CF

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