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Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10021-PL

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10021-PL-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse PD-L2/B7-DC protein
Mouse PD-L2
(Leu20-Arg219) (Lys113Ser)
Accession # Q9WUL5
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu20

Predicted Molecular Mass

49 kDa

SDS-PAGE

64-84 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse PD‑L2/B7‑DC (K113S) Fc Chimera is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse RGM‑B (Catalog # 3597-RG) binds with an ED50 of 0.08-0.48 μg/mL.
Recombinant Mouse PD‑L2/B7‑DC (K113S) Fc Chimera (Catalog #10021-PL) weakly binds to Recombinant Mouse PD-1.

Scientific Data Images for Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein, CF

Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein Binding Activity

Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein Binding Activity

When Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera (Catalog # 10021-PL) is immobilized at 2 µg/mL, Recombinant Mouse RGMB (Catalog # 3597-RG) binds with an ED50 of 0.08-0.48 µg/mL.
Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein SDS-PAGE

Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse PD‑L2/B7‑DC (K113S) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 64-84 kDa and 125-170 kDa, respectively.

Formulation, Preparation and Storage

10021-PL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: PD-L2/B7-DC

Programmed Death Ligand 2 (PD-L2), also known as Butyrophilin B7-DC, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1). In humans, the mature PD-L2 consists of a 201 amino acid (aa) extracellular domain (ECD) with one V-like and one C-like Ig domain, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2, 3). Within the ECD, mouse PD-L2 shares 72% and 95% aa sequence identity with human and rat PD‑L2, respectively. PD-L2 is expressed on dendritic cells, subsets of activated CD4+ and CD8+ T cells, and memory B cells that differentiate into plasma cells (3‑5). At inflammatory sites such as rheumatoid arthritis, allergen exposure, and virus infection, PD-L2 is up-regulated on synoviocytes, infiltrating macrophages, dendritic cells, and airway epithelial cells (6-10). PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand up-regulation while inhibiting IL-4 production (2, 3, 11, 12). In addition, PD-L2 binds to repulsive guidance molecule family member b (RGMb) on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance (13). Replacement of lysine residue at position 113 with serine (K113S) has been reported to result in a loss of binding capacity to PD-1, while retaining its T-cell stimulatory function through its interaction with RGMb (14). In asthma, PD-L2 suppresses IL-5 and IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production (8, 10).

References

  1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  2. Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
  3. Tseng, S.Y. et al. (2001) J. Exp. Med. 193:839.
  4. Messal, N. et al. (2011) Mol. Immunol. 48:2214.
  5. Zuccarino-Catania, G.V. et al. (2014) Nat. Immunol. 15:631.
  6. Guo, G. et al. (2012) Clin. Rheumatol. 31:271.
  7. Loke, P. and J.P. Allison (2003) Proc. Natl. Acad. Sci. USA 100:5336.
  8. Matsumoto, K. et al. (2004) J. Immunol. 172:2530.
  9. Stanciu, L.A. et al. (2006) J. Infec. Dis. 193:404.
  10. Lewkowich, I.P. et al. (2013) Mucosal Immun. 6:728.
  11. Ghiotto, M. et al. (2010) Int. Immunol. 22:651.
  12. Shin, T. et al. (2003) J. Exp. Med. 198:31.
  13. Xiao, Y. et al. (2014) J. Exp. Med. 211:943.
  14. Nie, X. et al. (2017) Cell. Mol. Immunol. 14:1.

Long Name

Programmed Death-1 Ligand-2

Alternate Names

B7-DC, Butyrophilin-like Protein, CD273, PDCD1LG2, PDL2

Entrez Gene IDs

80380 (Human); 58205 (Mouse); 309304 (Rat); 102146227 (Cynomolgus Monkey)

Gene Symbol

PDCD1LG2

UniProt

Additional PD-L2/B7-DC Products

Product Documents for Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein, CF

For research use only

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