Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein, CF

Name: Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10021-PL

Catalog # 10021-PL

Product Datasheet / COA / SDS

Order Information

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Key Product Details

Source:	NS0
Accession #:	Q9WUL5
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein, CF

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse PD-L2/B7-DC protein

Mouse PD-L2 (Leu20-Arg219) (Lys113Ser) Accession # Q9WUL5	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu20

Predicted Molecular Mass

49 kDa

SDS-PAGE

64-84 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Mouse PD‑L2/B7‑DC (K113S) Fc Chimera is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse RGM‑B (Catalog # 3597-RG) binds with an ED₅₀ of 0.08-0.48 μg/mL.

Recombinant Mouse PD‑L2/B7‑DC (K113S) Fc Chimera (Catalog #10021-PL) weakly binds to Recombinant Mouse PD-1.

Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein Binding Activity When Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera (Catalog # 10021-PL) is immobilized at 2 µg/mL, Recombinant Mouse RGMB (Catalog # 3597-RG) binds with an ED₅₀ of 0.08-0.48 µg/mL.
	Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Mouse PD‑L2/B7‑DC (K113S) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 64-84 kDa and 125-170 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10021-PL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PD-L2/B7-DC

Programmed Death Ligand 2 (PD-L2), also known as Butyrophilin B7-DC, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1). In humans, the mature PD-L2 consists of a 201 amino acid (aa) extracellular domain (ECD) with one V-like and one C-like Ig domain, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2, 3). Within the ECD, mouse PD-L2 shares 72% and 95% aa sequence identity with human and rat PD‑L2, respectively. PD-L2 is expressed on dendritic cells, subsets of activated CD4⁺ and CD8⁺ T cells, and memory B cells that differentiate into plasma cells (3‑5). At inflammatory sites such as rheumatoid arthritis, allergen exposure, and virus infection, PD-L2 is up-regulated on synoviocytes, infiltrating macrophages, dendritic cells, and airway epithelial cells (6-10). PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand up-regulation while inhibiting IL-4 production (2, 3, 11, 12). In addition, PD-L2 binds to repulsive guidance molecule family member b (RGMb) on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance (13). Replacement of lysine residue at position 113 with serine (K113S) has been reported to result in a loss of binding capacity to PD-1, while retaining its T-cell stimulatory function through its interaction with RGMb (14). In asthma, PD-L2 suppresses IL-5 and IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production (8, 10).

Item	Value
References	Ceeraz, S. et al. (2013) Trends Immunol. 34:556. Latchman, Y. et al. (2001) Nat. Immunol. 2:261. Tseng, S.Y. et al. (2001) J. Exp. Med. 193:839. Messal, N. et al. (2011) Mol. Immunol. 48:2214. Zuccarino-Catania, G.V. et al. (2014) Nat. Immunol. 15:631. Guo, G. et al. (2012) Clin. Rheumatol. 31:271. Loke, P. and J.P. Allison (2003) Proc. Natl. Acad. Sci. USA 100:5336. Matsumoto, K. et al. (2004) J. Immunol. 172:2530. Stanciu, L.A. et al. (2006) J. Infec. Dis. 193:404. Lewkowich, I.P. et al. (2013) Mucosal Immun. 6:728. Ghiotto, M. et al. (2010) Int. Immunol. 22:651. Shin, T. et al. (2003) J. Exp. Med. 198:31. Xiao, Y. et al. (2014) J. Exp. Med. 211:943. Nie, X. et al. (2017) Cell. Mol. Immunol. 14:1.
Long Name	Programmed Death-1 Ligand-2
Entrez Gene IDs	80380 (Human); 58205 (Mouse); 309304 (Rat); 102146227 (Cynomolgus Monkey)
Alternate Names	B7-DC, Btdc, Butyrophilin B7-DC, Butyrophilin-like Protein, CD273, CD273 antigen, CD273PD-1 ligand 2, MGC142240, PD-1-ligand 2, PD-L2, PD-L2PDCD1 ligand 2, PDCD1L2MGC142238, PDCD1LG2, PDL2, PDL2B7DC, Programmed death ligand 2, bA574F11.2, programmed cell death 1 ligand 2

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