Recombinant Mouse RGM-B Protein, CF

Catalog # 3597-RG | R&D Systems, Inc. a Bio-Techne Brand
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3597-RG-050
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Key Product Details

Accession #

Q7TQ33

Source

NS0

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse RGM-B protein
Gly49-Asp171 (N-terminus chain) & Pro172-Ser414 (C-terminus chain), with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly49 (N-terminus chain) & Pro172 (C-terminus chain)

Predicted Molecular Mass

13.3 kDa (N-terminus chain), 27.8 kDa (C-terminus chain)

SDS-PAGE

20 kDa and 27 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immoblized rhBMP-4 at 1 µg/mL (100 µL/well) can bind rmRGM-B with a linear range of 0.016‑1 µg/mL.

Formulation, Preparation and Storage

3597-RG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RGM-B

RGM-B, also known as DRAGON, is a 40 kDa member of the repulsive guidance molecule (RGM) family of GPI-linked neuronal and muscle membrane proteins (1, 2). It is synthesized as a preproprotein that consists of a 48 amino acid (aa) signal sequence, a 367 aa mature region, and a 21 aa C-terminal prosegment (3). RGM-B contains two potential N-linked glycosylation sites and an abbreviated von Willebrand factor domain. Potential proteolytic cleavage within the VWF domain is supported by R&D Systems’ in house data (4). Within the region following the VWF domain, mouse RGM-B shares 49% and 43% aa sequence identity with RGM-A and RGM-C, respectively. It shares 90%, 79%, 92%, and 93% aa sequence identity with bovine, chicken, human, and macaque RGM-B, respectively. RGM-B is expressed in the developing and adult nervous system, particularly in the dorsal root ganglia and mantle layer of the spinal cord (3 - 5). In mouse, it shows a complementary, non-overlapping distribution with RGM-A (2 - 5). RGM-B is also expressed in fetal and adult enteric ganglia and in postnatal intestinal epithelium (6). RGM-B expression has been detected in neuronal cell bodies and proximal axonal segments (4) but is also present on the cell surface, where it interacts homophilically and mediates neuronal adhesion (3). RGM-B additionally functions as a BMP coreceptor. It directly binds BMP-2 and -4 but not other TGF-beta family proteins (7). RGM-B associates with BMP type I (ALK-2, -3, -6) and type II (Activin RIIA, Activin RIIB) receptors and enhances BMP signaling (7).

References

  1. Monnier, P.P. et al. (2002) Nature 419:392.
  2. Schmidtmer, J. and D. Engelkamp (2004) Gene Exp. Patterns 4:105.
  3. Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
  4. Niederkofler, V. et al. (2004) J. Neurosci. 24:808.
  5. Oldekamp, J. et al. (2004) Gene Exp. Patterns 4:283.
  6. Metzger, M. et al. (2005) Dev. Dyn. 234:169.
  7. Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.

Long Name

Repulsive Guidance Molecule B

Alternate Names

Dragon, RGMB

Entrez Gene IDs

285704 (Human); 68799 (Mouse)

Gene Symbol

RGMB

UniProt

Q7TQ33

Product Documents for Recombinant Mouse RGM-B Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse RGM-B Protein, CF

For research use only

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