Recombinant Mouse RGM-B Protein, CF
Catalog # 3597-RG | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Product Specifications
Source
Gly49-Asp171 (N-terminus chain) & Pro172-Ser414 (C-terminus chain), with a C-terminal 6-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Immoblized rhBMP-4 at 1 µg/mL (100 µL/well) can bind rmRGM-B with a linear range of 0.016‑1 µg/mL.
Formulation, Preparation and Storage
3597-RG
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
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Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: RGM-B
RGM-B, also known as DRAGON, is a 40 kDa member of the repulsive guidance molecule (RGM) family of GPI-linked neuronal and muscle membrane proteins (1, 2). It is synthesized as a preproprotein that consists of a 48 amino acid (aa) signal sequence, a 367 aa mature region, and a 21 aa C-terminal prosegment (3). RGM-B contains two potential N-linked glycosylation sites and an abbreviated von Willebrand factor domain. Potential proteolytic cleavage within the VWF domain is supported by R&D Systems’ in house data (4). Within the region following the VWF domain, mouse RGM-B shares 49% and 43% aa sequence identity with RGM-A and RGM-C, respectively. It shares 90%, 79%, 92%, and 93% aa sequence identity with bovine, chicken, human, and macaque RGM-B, respectively. RGM-B is expressed in the developing and adult nervous system, particularly in the dorsal root ganglia and mantle layer of the spinal cord (3 - 5). In mouse, it shows a complementary, non-overlapping distribution with RGM-A (2 - 5). RGM-B is also expressed in fetal and adult enteric ganglia and in postnatal intestinal epithelium (6). RGM-B expression has been detected in neuronal cell bodies and proximal axonal segments (4) but is also present on the cell surface, where it interacts homophilically and mediates neuronal adhesion (3). RGM-B additionally functions as a BMP coreceptor. It directly binds BMP-2 and -4 but not other TGF-beta family proteins (7). RGM-B associates with BMP type I (ALK-2, -3, -6) and type II (Activin RIIA, Activin RIIB) receptors and enhances BMP signaling (7).
References
- Monnier, P.P. et al. (2002) Nature 419:392.
- Schmidtmer, J. and D. Engelkamp (2004) Gene Exp. Patterns 4:105.
- Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
- Niederkofler, V. et al. (2004) J. Neurosci. 24:808.
- Oldekamp, J. et al. (2004) Gene Exp. Patterns 4:283.
- Metzger, M. et al. (2005) Dev. Dyn. 234:169.
- Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Recombinant Mouse RGM-B Protein, CF
Product Specific Notices for Recombinant Mouse RGM-B Protein, CF
For research use only
Citations for Recombinant Mouse RGM-B Protein, CF (3)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse RGM-B Protein, CF include:
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Species: Mouse
Sample Types: Recombinant Protein
Applications: Surface Plasmon ResonanceX Nie et al. (2017-05-08), B7-DC (PD-L2) costimulation of CD4(+) T-helper 1 response via RGMb Cell. Mol. Immunol., 2017-00(0).
PMID: 28479601 -
Species: Mouse,Rat
Sample Types: Antibody,In Vivo
Applications: ELISA (Capture),In VivoXiao et al. (2014-04-21), RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance. J Exp Med, 2014-0211(5):943-59.
PMID: 24752301 -
Species: Rat
Sample Types: Whole Cells
Applications: BioassayLiu X et al. (2009-03-24), Repulsive guidance molecule b inhibits neurite growth and is increased after spinal cord injury. Biochem. Biophys. Res. Commun., 2009-0382(4):795-800.
PMID: 19324014
There are no citations that match your criteria.
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