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Recombinant Mouse PD-L2/B7-DC Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1022-PL

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1022-PL-01M
1022-PL-100

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse PD-L2/B7-DC protein
Mouse PD-L2
(Leu20-Arg219)
Accession # Q9WUL5
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu20

Predicted Molecular Mass

49 kDa (monomer)

SDS-PAGE

76 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated mouse T cells.
The ED50 for this effect is 1-6 µg/mL.

Scientific Data Images for Recombinant Mouse PD-L2/B7-DC Fc Chimera Protein, CF

Recombinant Mouse PD-L2/B7-DC Fc Chimera Protein Bioactivity

Recombinant Mouse PD-L2/B7-DC Fc Chimera Protein Bioactivity

Recombinant Mouse PD-L2/B7-DC Fc Chimera (Catalog # 1022-PL) inhibits anti-CD3 induced T cell proliferation. The ED50 for this effect is 1-6 μg/mL.

Formulation, Preparation and Storage

1022-PL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 400 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-L2/B7-DC

Programmed Death Ligand 2 (PD-L2), also known as B7-DC and butyrophilin-like protein, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1). Mature mouse PD-L2 consists of a 199 amino acid (aa) extracellular domain (ECD) with one V-like and one C-like Ig domain, a 23 aa transmembrane segment, and a 5 aa cytoplasmic domain (2, 3). Within the ECD, mouse and human PD-L2 share 72% aa sequence identity. PD-L2 is expressed on dendritic cells, subsets of activated CD4+ and CD8+ T cells, and memory B cells that differentiate into plasma cells (3-5). At inflammatory sites such as rheumatoid arthritis, allergen exposure, and virus infection, PD-L2 is up-regulated on synoviocytes, infiltrating macrophages, dendritic cells, and airway epithelial cells (6-10).
PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand up-regulation while inhibiting IL-4 production (2, 3, 11, 12). In addition, PD-L2 binds to RGM-B on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance (13). In asthma, PD-L2 suppresses IL-5 and
IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production (8, 10).

References

  1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  2. Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
  3. Tseng, S.-Y. et al. (2001) J. Exp. Med. 193:839.
  4. Messal, N. et al. (2011) Mol. Immunol. 48:2214.
  5. Zuccarino-Catania, G.V. et al. (2014) Nat. Immunol. 15:631.
  6. Guo, G. et al. (2012) Clin. Rheumatol. 31:271.
  7. Loke, P. and J.P. Allison (2003) Proc. Natl. Acad. Sci. USA 100:5336.
  8. Matsumoto, K. et al. (2004) J. Immunol. 172:2530.
  9. Stanciu, L.A. et al. (2006) J. Infec. Dis. 193:404.
  10. Lewkowich, I.P. et al. (2013) Mucosal Immun. 6:728.
  11. Ghiotto, M. et al. (2010) Int. Immunol. 22:651.
  12. Shin, T. et al. (2003) J. Exp. Med. 198:31.
  13. Xiao, Y. et al. (2014) J. Exp. Med. 211:943.

Long Name

Programmed Death-1 Ligand-2

Alternate Names

B7-DC, Butyrophilin-like Protein, CD273, PDCD1LG2, PDL2

Entrez Gene IDs

80380 (Human); 58205 (Mouse); 309304 (Rat); 102146227 (Cynomolgus Monkey)

Gene Symbol

PDCD1LG2

UniProt

Additional PD-L2/B7-DC Products

Product Documents for Recombinant Mouse PD-L2/B7-DC Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse PD-L2/B7-DC Fc Chimera Protein, CF

For research use only

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