Recombinant Mouse PD-L2/B7-DC Fc Chimera Protein, CF

Name: Recombinant Mouse PD-L2/B7-DC Fc Chimera Protein, CF
Brand: rnd
SKU: 1022-PL
Rating: 5 (1 reviews)

Catalog # 1022-PL

Citations (3)

(1)

Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	Q9WUL5
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse PD-L2/B7-DC protein

Mouse PD-L2 (Leu20-Arg219) Accession # Q9WUL5	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu20

Predicted Molecular Mass

49 kDa (monomer)

SDS-PAGE

76 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated mouse T cells. The ED₅₀ for this effect is 1-6 µg/mL.

Recombinant Mouse PD-L2/B7-DC Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Mouse PD-L2/B7-DC Fc Chimera Protein Bioactivity Recombinant Mouse PD-L2/B7-DC Fc Chimera (Catalog # 1022-PL) inhibits anti-CD3 induced T cell proliferation. The ED₅₀ for this effect is 1-6 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1022-PL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 400 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PD-L2/B7-DC

Programmed Death Ligand 2 (PD-L2), also known as B7-DC and butyrophilin-like protein, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1). Mature mouse PD-L2 consists of a 199 amino acid (aa) extracellular domain (ECD) with one V-like and one C-like Ig domain, a 23 aa transmembrane segment, and a 5 aa cytoplasmic domain (2, 3). Within the ECD, mouse and human PD-L2 share 72% aa sequence identity. PD-L2 is expressed on dendritic cells, subsets of activated CD4⁺ and CD8⁺ T cells, and memory B cells that differentiate into plasma cells (3-5). At inflammatory sites such as rheumatoid arthritis, allergen exposure, and virus infection, PD-L2 is up-regulated on synoviocytes, infiltrating macrophages, dendritic cells, and airway epithelial cells (6-10).
PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand up-regulation while inhibiting IL-4 production (2, 3, 11, 12). In addition, PD-L2 binds to RGM-B on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance (13). In asthma, PD-L2 suppresses IL-5 and
IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production (8, 10).

Item	Value
References	Ceeraz, S. et al. (2013) Trends Immunol. 34:556. Latchman, Y. et al. (2001) Nat. Immunol. 2:261. Tseng, S.-Y. et al. (2001) J. Exp. Med. 193:839. Messal, N. et al. (2011) Mol. Immunol. 48:2214. Zuccarino-Catania, G.V. et al. (2014) Nat. Immunol. 15:631. Guo, G. et al. (2012) Clin. Rheumatol. 31:271. Loke, P. and J.P. Allison (2003) Proc. Natl. Acad. Sci. USA 100:5336. Matsumoto, K. et al. (2004) J. Immunol. 172:2530. Stanciu, L.A. et al. (2006) J. Infec. Dis. 193:404. Lewkowich, I.P. et al. (2013) Mucosal Immun. 6:728. Ghiotto, M. et al. (2010) Int. Immunol. 22:651. Shin, T. et al. (2003) J. Exp. Med. 198:31. Xiao, Y. et al. (2014) J. Exp. Med. 211:943.
Long Name	Programmed Death-1 Ligand-2
Entrez Gene IDs	80380 (Human); 58205 (Mouse); 309304 (Rat); 102146227 (Cynomolgus Monkey)
Alternate Names	B7-DC, Btdc, Butyrophilin B7-DC, Butyrophilin-like Protein, CD273, CD273 antigen, CD273PD-1 ligand 2, MGC142240, PD-1-ligand 2, PD-L2, PD-L2PDCD1 ligand 2, PDCD1L2MGC142238, PDCD1LG2, PDL2, PDL2B7DC, Programmed death ligand 2, bA574F11.2, programmed cell death 1 ligand 2

Citations (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse PD-L2/B7-DC Fc Chimera Protein, CF include:

Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

LP Garo et al. (2019), Smad7 Controls Immunoregulatory PDL2/1-PD1 Signaling in Intestinal Inflammation and Autoimmunity Cell Rep, 28(13):3353-3366.e5.
PMID: 31553906
Species: Mouse
Sample Types: Antibody
Applications: ELISA (Capture)

Xiao et al. (2014), RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance. J Exp Med, 211(5):943-59.
PMID: 24752301
Species: N/A
Sample Types: N/A
Applications: Surface Plasmon Resonance

Butte MJ et al. (2007), Programmed death-1 ligand 1 interacts specifically with the B7-1 costimulatory molecule to inhibit T cell responses. Immunity, 27(1):111-22.
PMID: 17629517

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Recombinant Mouse PD-L2/B7-DC Fc Chimera Protein, CF

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Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 11/30/2017

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Product Documents

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Recombinant Mouse PD-L2/B7-DC Fc Chimera Protein, CF

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Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications

Recombinant Mouse PD-L2/B7-DC Fc Chimera Protein, CF Scientific Data Examples

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: PD-L2/B7-DC

Citations (3)

Customer Reviews (1)

Recombinant Mouse PD-L2/B7-DC Fc Chimera Protein, CF

Product Documents

Product Datasheets

COA

Certificate of Analysis

SDS

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