Recombinant Mouse P-Selectin/CD62P His-tag Protein, CF

Name: Recombinant Mouse P-Selectin/CD62P His-tag Protein, CF
Brand: rnd
SKU: 10094-PS

Catalog # 10094-PS

Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	Q01102
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse P-Selectin/CD62P His-tag Protein, CF

Product Specifications

Source	Mouse myeloma cell line, NS0-derived mouse P-Selectin/CD62P protein Trp42-Ala709, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Trp42
Predicted Molecular Mass	73.4 kDa
SDS-PAGE	105-125 kDa, reducing conditions
Activity	Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. The ED₅₀ for this effect is 0.15-0.9 μg/mL.

Recombinant Mouse P-Selectin/CD62P His-tag Protein, CF Scientific Data Examples

	Recombinant Mouse P-Selectin/CD62P His-tag Protein Bioactivity Immobilized Recombinant Mouse P‑Selectin/CD62P His-tag (Catalog # 10094-PS) supports the adhesion of U937 human histiocytic lymphoma cells. The ED₅₀ for this effect is 0.15‑0.9 μg/mL.
	Recombinant Mouse P-Selectin/CD62P His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Mouse P‑Selectin/CD62P His-tag (Catalog # 10094-PS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-125 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10094-PS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: P-Selectin/CD62P

P-Selectin, also known as GMP-140, LECAM-3, PADGEM, and CD62P, is a cell surface glycoprotein of the Selectin family and is expressed by activated platelets and endothelial cells (1-3). Mouse P-Selectin cDNA encodes a 768 amino acid (aa) type I transmembrane protein with a signal peptide, a large extracellular domain (ECD), a transmembrane domain and a short cytoplasmic domain (4). The mature ECD of mouse P-Selectin contains a C-type lectin domain and an EGF-like domain followed by a series of complement factor A repeat homology domains (5). The mature ECD of mouse P-selectin shares 66% and 89% aa sequence identity with human and rat, respectively. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides (6). P-Selectin binds to P-Selectin glycoprotein ligand-1 (PSGL-1), a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes (7). P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling' of the leukocytes on the endothelium (8). This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue (1, 3).

Item	Value
References	Kansas, G.S. (1996) Blood 88:3259. Lü, S. (2015) PLOS One 10:1. Edwards, E.E. (2017) Integr Biol (Camb) 9:313. Patel, K.D. (1995) J. Cell Biol. 131:1893. McEver, R.P. (1997) J. Clin. Invest. 100:485. Zhang, N. (2016) Arterioscler Thromb Vasc Biol 36:1114. Luo, W. (2012) Atherosclerosis 220:110. Zuchtriegel, G. (2016) PLOS Biology 14:1.
Entrez Gene IDs	6403 (Human); 20344 (Mouse)
Alternate Names	CD62P, CD62P antigen, FLJ45155, GMP140, GRMP, P-Selectin, PADGEM, PADGEMantigen CD62), PSEL, SELP, selectin P (granule membrane protein 140kDa, antigen CD62)

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