Recombinant Mouse Osteopontin/OPN Protein

Name: Recombinant Mouse Osteopontin/OPN Protein
Brand: R&D Systems
SKU: 441-OP
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 441-OP

Citations (33)

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Key Product Details

Source:	NS0
Accession #:	Q547B5
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse Osteopontin/OPN Protein

Product Specifications for Recombinant Mouse Osteopontin/OPN Protein

Source	Mouse myeloma cell line, NS0-derived mouse Osteopontin/OPN protein Leu17-Asn294 (Glu99Gly), with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu17
Predicted Molecular Mass	31.5 kDa
SDS-PAGE	30 kDa and a 65 kDa doublet, reducing conditions
Activity	Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261. When 1 x 10⁵ cells/well are added to a Recombinant Mouse (rm) Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 °C. The ED₅₀for this effect is 0.25-1.5 µg/mL. Recombinant Human Coagulation Factor II/Thrombin (Catalog # 1473-SE) proteolytic treatment of this rmOsteopontin can increase HEK293 cell adhesion by about 10-fold.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

441-OP

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

441-OP/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Osteopontin/OPN

Osteopontin (OPN), previously called SPP1 (secreted phosphoprotein 1), Eta-1 (early T lymphocyte activation 1) or BSP (bone sialoprotein), is a secreted molecule in the SIBLING (small integrin-binding ligand N-linked glycoprotein) family of non-collagenous matricellular proteins (1-3). Mouse OPN is synthesized as a 294 amino acid (aa) precursor protein with a 16 aa signal peptide and a 278 aa mature protein (3). Mature mouse OPN shares 79% and 64% aa sequence identity with rat and human OPN, respectively. OPN is highly acidic and has 26 potential Ser/Thr phosphorylation sites and a C‑terminal CD44 binding site (1-4). Depending on tissue-specific modification by O- and N-glycosylation, sulfation, phosphorylation and transglutamination, OPN can be detected at 45-75 kDa (5, 6). The central region of OPN contains RGD and non-RGD binding sites for multiple integrins (3, 4). Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by thrombin in tumors and sites of tissue injury
(6-8). OPN can also be cleaved by MMP-3, -7, -9, and -12 within the SLAYGLR motif and at sites closer to the C-terminus (8, 9). OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation, and promotes inflammation and cell adhesion and migration (1, 2, 4, 6). Its expression is up-regulated during inflammation, obesity, atherosclerosis, cancer, and tissue damage, and contributes to the pathophysiology of these conditions (1, 2, 6, 9, 10).

Item	Value
References	Scatena, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:2302. Rangaswami, H. et al. (2006) Trends Cell Biol. 16:79. Miyazaki, Y. et al. (1990) J. Biol. Chem. 265:14432. Weber, G.F. et al. (2002) J. Leukoc. Biol. 72:752. Keykhosravani, M. et al. (2005) Biochemistry 44:6990. Kazanecki, C.C. et al. (2007) J. Cell. Biochem. 102:912. Senger, D.R. et al. (1994) Mol. Biol. Cell 5:565. Yokosaki, Y. et al. (2005) Matrix Biol. 24:418. Takafuji, V. et al. (2007) Oncogene 26:6361. Kiefer, F.W. et al. (2010) Diabetes 59:935.
Long Name	Secreted Phosphoprotein 1 [BNSP]
Entrez Gene IDs	6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
Alternate Names	BNSP, Bone sialoprotein 1, Eta-1, MGC110940, Nephropontin, OPN, Osteopontin, SPP-1, SPP1/CALPHA1 fusion, Spp1, Urinary stone protein, secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1), secreted phosphoprotein-1 (osteopontin, bone sialoprotein), uropontin

Citations for Recombinant Mouse Osteopontin/OPN Protein (33)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Osteopontin/OPN Protein include:

Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

FZ Khamissi et al. (2022), Identification of kidney injury released circulating osteopontin as causal agent of respiratory failure Science Advances, 8(8):eabm5900.
PMID: 35213222
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay

S Makishi et al. (2022), Osteopontin on the Dental Implant Surface Promotes Direct Osteogenesis in Osseointegration International Journal of Molecular Sciences, 23(3).
PMID: 35162963
Species: Human
Sample Types: Whole Cell
Applications: Cell Culture

CL Hong et al. (2022), CD248 regulates Wnt signaling in pericytes to promote angiogenesis and tumor growth in lung cancer Cancer Research, 0(0).
PMID: 35950912
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

J Chen et al. (2022), Osteopontin Exacerbates High-Fat Diet-Induced Metabolic Disorders in a Microbiome-Dependent Manner MBio, 0(0):e0253122.
PMID: 36300928
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

D Raineri et al. (2021), Inducible T-Cell Costimulator Ligand Plays a Dual Role in Melanoma Metastasis upon Binding to Osteopontin or Inducible T-Cell Costimulator Biomedicines, 10(1).
PMID: 35052731
Species: Mouse
Sample Types: Serum
Applications: Western Blot Control

S Yoshida et al. (2020), The CD153 vaccine is a senotherapeutic option for preventing the accumulation of senescent T cells in mice Nat Commun, 11(1):2482.
PMID: 32424156
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

KM Herum et al. (2020), Syndecan-4 Protects the Heart From the Profibrotic Effects of Thrombin-Cleaved Osteopontin J Am Heart Assoc, 9(3):e013518.
PMID: 32000579
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

PG Hitscheric et al. (2019), The effects of macrophages on cardiomyocyte calcium-handling function using in vitro culture models Physiol Rep, 7(13):e14137.
PMID: 31301118
Species: Mouse

Applications: In Vivo

G Zhao et al. (2018), Osteopontin contributes to effective neutrophils recruitment, IL-1? production, and apoptosis in Aspergillus fumigatus keratitis Immunol. Cell Biol., 0(0).
PMID: 29359350
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

R Rogall et al. (2018), Bioluminescence imaging visualizes osteopontin-induced neurogenesis and neuroblast migration in the mouse brain after stroke Stem Cell Res Ther, 9(1):182.
PMID: 29973246
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Q Zeng et al. (2018), Leptin/Osteopontin Axis Regulated Type 2T Helper Cell Response in Allergic Rhinitis with Obesity EBioMedicine, 0(0).
PMID: 29885866
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Bioassay

RM Natoli et al. (2018), Alcohol exposure decreases osteopontin expression during fracture healing and osteopontin-mediated mesenchymal stem cell migration in vitro J Orthop Surg Res, 13(1):101.
PMID: 29699560
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

MB Alvarez et al. (2018), Megakaryocyte and Osteoblast Interactions Modulate Bone Mass and Hematopoiesis Stem Cells Dev., 0(0).
PMID: 29631496
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

W Wang et al. (2017), Osteopontin activates mesenchymal stem cells to repair skin wound PLoS ONE, 12(9):e0185346.
PMID: 28957406
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

AM Paul et al. (2017), Osteopontin facilitates West Nile virus neuroinvasion via neutrophil Trojan horse transport Sci Rep, 7(1):4722.
PMID: 28680095
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

M Quattrocel et al. (2017), Genetic modifiers of muscular dystrophy act on sarcolemmal resealing and recovery from injury PLoS Genet., 13(10):e1007070.
PMID: 29065150
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

CH Chen et al. (2017), Oligo-fucoidan prevents renal tubulointerstitial fibrosis by inhibiting the CD44 signal pathway Sci Rep, 7(0):40183.
PMID: 28098144
Species: Mouse
Sample Types: Protein
Applications: Western Blot

Y Kamioka et al. (2017), Intravital Fï¿½rster resonance energy transfer imaging reveals osteopontin-mediated polymorphonuclear leukocyte activation by tumor cell emboli Cancer Sci, 108(2):226-235.
PMID: 27960041
Species: Feline
Sample Types: Urine
Applications: Bioassay

(2016), Nanoparticle Tracking Analysis for the Enumeration and Characterization of Mineralo-Organic Nanoparticles in Feline Urine PLoS ONE, 11(12):e0166045.
PMID: 28005930
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Richard P Ciavarra (2016), Early gene activation initiates neuroinflammation prior to VSV neuroinvasion: Impact on antiviral responses and sleep J. Neuroimmunol, 0(0).
PMID: 28041664
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Yuta Morisaki (2016), Selective Expression of Osteopontin in ALS-resistant Motor Neurons is a Critical Determinant of Late Phase Neurodegeneration Mediated by Matrix Metalloproteinase-9 Sci Rep, 6(0):27354.
PMID: 27264390
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2016), Osteopontin in Spontaneous Germinal Centers Inhibits Apoptotic Cell Engulfment and Promotes Anti-Nuclear Antibody Production in Lupus-Prone Mice J Immunol, 0(0).
PMID: 27534552
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Maruyama K et al. (2015), 5-Azacytidine-induced protein 2 (AZI2) regulates bone mass by fine-tuning osteoclast survival. J Biol Chem, 290(15):9377-86.
PMID: 25691576
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Qi et al. (2014), Osteopontin is expressed in the mouse uterus during early pregnancy and promotes mouse blastocyst attachment and invasion in vitro. PLoS ONE, 9(8):e104955.
PMID: 25133541
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Chen Q et al. (2014), An osteopontin-integrin interaction plays a critical role in directing adipogenesis and osteogenesis by mesenchymal stem cells. Stem Cells, 32(2):327-37.
PMID: 24123709
Kourepini E et al. (2014), Osteopontin expression by CD103- dendritic cells drives intestinal inflammation. Proc Natl Acad Sci U S A, 111(9):E856-65.
PMID: 24550510
Species: Human
Sample Types: Antibody
Applications: Surface Plasmon Resonance

Shojaei F et al. (2012), Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer. J. Exp. Clin. Cancer Res., 31(0):26.
PMID: 22444159
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Dahiya S et al. (2011), Osteopontin-Stimulated Expression of Matrix Metalloproteinase-9 Causes Cardiomyopathy in the mdx Model of Duchenne Muscular Dystrophy. J. Immunol., 187(5):2723-31.
PMID: 21810612
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Schneider DJ et al. (2010), Adenosine and osteopontin contribute to the development of chronic obstructive pulmonary disease. FASEB J., 24(1):70-80.
PMID: 19720619
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Marcondes MC et al. (2008), In vivo osteopontin-induced macrophage accumulation is dependent on CD44 expression. Cell. Immunol., 254(1):56-62.
PMID: 18678363
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Xanthou G et al. (2007), Osteopontin has a crucial role in allergic airway disease through regulation of dendritic cell subsets. Nat. Med., 13(5):570-8.
PMID: 17435770
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Jacobs JP et al. (2004), Lack of requirement of osteopontin for inflammation, bone erosion, and cartilage damage in the K/BxN model of autoantibody-mediated arthritis. Arthritis Rheum., 50(8):2685-94.
PMID: 15334485
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Bruemmer D et al. (2003), Angiotensin II-accelerated atherosclerosis and aneurysm formation is attenuated in osteopontin-deficient mice. J. Clin. Invest., 112(9):1318-31.
PMID: 14597759

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Recombinant Mouse Osteopontin/OPN Protein

Name: Cuiping Zhang

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 11/13/2020

Recombinant Mouse Osteopontin/OPN Protein

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Product Specifications for Recombinant Mouse Osteopontin/OPN Protein

Formulation, Preparation and Storage

Carrier Free

441-OP

Carrier

441-OP/CF

Carrier Free

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Background: Osteopontin/OPN

Citations for Recombinant Mouse Osteopontin/OPN Protein (33)

Customer Reviews for Recombinant Mouse Osteopontin/OPN Protein (1)

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Recombinant Mouse Osteopontin/OPN Protein

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