Recombinant Mouse Notch-1 Fc Chimera Protein, CF

Name: Recombinant Mouse Notch-1 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 5267-TK

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 5267-TK

Citations (3)

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Key Product Details

Source:	CHO
Accession #:	AAM28905
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

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Product Specifications for Recombinant Mouse Notch-1 Fc Chimera Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Notch-1 protein

Mouse Notch-1 (Ala19 - Gln526) Accession # AAM28905	IEGRMDP	Mouse IgG_2A (Glu98 - Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala19

Predicted Molecular Mass

80.7 kDa (monomer)

SDS-PAGE

110-120 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse Notch-1 Fc Chimera at 2 μg/mL (100 μL/mL) can bind Recombinant Rat Jagged 1 Fc Chimera (Catalog # 599-JG) with an apparent K_D< 2 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5267-TK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Notch-1

Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four Notch homologues involved in developmental processes (1 - 3). Notch signaling is important for maintaining stem cells and inducing differentiation, especially in the nervous system and lymphoid tissues (2 - 4). Notch can specify binary cell fates. For example, it promotes T-cell over B-cell development from a common precursor (2). Mouse Notch-1 is synthesized as a 2531 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1707 aa extracellular domain (ECD) with 36 EGF-like repeats and three Lin‑12/notch repeats (LNR), a 21 aa transmembrane (TM) segment and a 785 aa cytoplasmic domain that contains six ankyrin repeats, a glutamine-rich domain and a PEST sequence. The 11^th and 12^th EGF-like repeats, that bind ligands such as Jagged and Delta‑like families in humans, correspond to aa 412 - 488 in mouse Notch-1 (6). Elongation of O‑linked fucose chains by Fringe family members at a site within this region can inhibit the interaction of Notch with Jagged ligands, thereby promoting Delta‑like ligand interactions (7). The Notch-1 receptor undergoes post-translational furin-type proteolytic cleavage, generating a heterodimer through the interaction of a hydrophobic area C‑terminal to the LNR on the extracellular region with the transmembrane/cytoplasmic portion (8, 9). Upon ligand binding, additional sequential proteolysis by TNF-converting enzyme (ADAM17) and the presenilin-dependent gamma -secretase results in the release of the Notch intracellular domain (NICD) which translocates into the nucleus, activating transcription of Notch-responsive genes (10). Mouse Notch-1 ECD aa 19 - 526, which includes the first 13 EGF repeats, shows 94%, 91%, 86% and 79% aa identity with corresponding regions of rat, human, canine, and chicken Notch-1, respectively. This region also exhibits 55 - 58% aa identity with human Notch‑2 and Notch-3.

Item	Value
References	Ellisen, L.W. et al. (1991) Cell 66:649. Dumortier, A. et al. (2005) Int. J. Hematol. 82:277. Yoon, K. and N. Gaiano (2005) Nat. Neurosci. 8:709. Androutsellis-Theotokis, A. et al. (2006) Nature 442:823. Weng, A.P. et al. (2004) Science 306:269. Hambleton, S. et al. (2004) Structure 12:2173. Yang, L. et al. (2005) Mol. Biol. Cell 16:927. Sanchez-Irizarry, C. et al. (2004) Mol. Cell. Biol. 24:9265. Logeat, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:8108. Mumm, J.S. and R. Kopan (2000) Dev. Biol. 228:151.
Entrez Gene IDs	4851 (Human); 25496 (Rat)
Alternate Names	EC 2.1.2.11, EC 3.4.21.68, Notch (Drosophila) homolog 1 (translocation-associated), Notch homolog 1, translocation-associated, Notch-1, Notch1, TAN1, TAN1neurogenic locus notch homolog protein 1, Translocation-associated notch protein TAN-1, hN1, notch 1Notch homolog 1, translocation-associated (Drosophila)

Citations for Recombinant Mouse Notch-1 Fc Chimera Protein, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Notch-1 Fc Chimera Protein, CF include:

Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

N Nandagopal et al. (2018), Dynamic Ligand Discrimination in the Notch Signaling Pathway Cell, 172(4):869-880.e19.
PMID: 29398116
Species: Hamster
Sample Types: Whole Cells
Applications: Bioassay

LeBon et al. (2014), Fringe proteins modulate Notch-ligand cis and trans interactions to specify signaling states. Elife, 3(0):e02950.
PMID: 25255098
Species: N/A
Sample Types: Protein
Applications: Bioassay

Benner et al. (2013), Protective astrogenesis from the SVZ niche after injury is controlled by Notch modulator Thbs4. Nature, 497(7449):369-73.
PMID: 23615612

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Recombinant Mouse Notch-1 Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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