Recombinant Mouse Norrin Protein

Name: Recombinant Mouse Norrin Protein
Brand: R&D Systems
SKU: 3497-NR
Price: 453 USD

Catalog # 3497-NR | R&D Systems, Inc. a Bio-Techne Brand

Citations (2)

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Key Product Details

Source:	E. coli
Accession #:	P48744
Structure / Form:	Disulfide-linked oligomer
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse Norrin Protein

Source	E. coli-derived mouse Norrin protein Lys25-Ser131, with an N-terminal Met
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	12.3 kDa (monomer)
Activity	Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells expressing human Frizzled-4 and human LRP-5. The ED₅₀for this effect is 40-200 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

3497-NR

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3497-NR/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Norrin

Norrin (also called Norrie Disease Protein or NDP) is a secreted regulatory protein that remains tightly associated with the extracellular matrix (1). At least 70 mutations of human Norrin have been identified in Norrie disease (X-linked retinal dysplasia) or in a minority of X-linked familial exudative vitreoretinopathy (FEVR). Both are disorders of retinal vascularization that cause blindness (2, 3). Norrin consists of disulfide-linked homodimers that oligomerize further via disulfide bridges to form higher order oligomers containing up to ten 12 kDa units. The cysteine-rich C-terminal domain of Norrin shows homology to von Willebrand factor, several extracellular mucin proteins, and members of the TGF-beta family. Molecular modeling studies predict that Norrin assumes the cysteine-knot structure typical of the TGF-beta family (4). Although Norrin is not related to Wnt family proteins, it functions like a Wnt protein in that it binds with high affinity to the receptor Frizzled-4, requires LDL receptor-related protein (LRP) as a co-receptor, and induces activation of the canonical Wnt signaling pathway (5). Norrin and Frizzled-4 are widely expressed, but at relatively low levels, in tissues displaying vascular phenotypes. Genetic disruption of either Norrin or Frizzled-4 results in blindness and progressive deafness due to vascular abnormalties (5-7). Female Norrin^-/- mice are infertile due to abnormalities in decidual vasculature (8). Mature mouse Norrin shares 97-98% aa identity with human, canine and bovine Norrin.

Item	Value
References	Perez-Vilar, J. and R.L. Hill (1997) J. Biol. Chem. 272:33410. Berger, W. et al. (1992) Nat. Genet. 1:199. Berger, W. and H.H. Ropers, 2001, in “The Metabolic and Molecular Bases of Inherited Diseases”, C.R. Scriver, et al., eds., pp. 5977. Meitinger, T. et al. (1993) Nat. Genet. 5:376. Xu, Q. et al. (2004) Cell 116:883. Hartzer, M.K. et al. (1999) Brain Res. Bull. 49:355. Wang, Y. et al. (2001) J. Neurosci. 21:4761. Luhmann, U.F.O. et al. (2005) Genesis 42:253.
Entrez Gene IDs	4693 (Human)
Alternate Names	EVR2, FEVR, ND, NDP, Norrie disease (pseudoglioma), Norrie disease protein, Norrin, X-linked exudative vitreoretinopathy 2 protein, exudative vitreoretinopathy 2 (X-linked)

Citations for Recombinant Mouse Norrin Protein (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Norrin Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Strakova et al. (2017-06-29), The tyrosine Y250(2.39) in Frizzled 4 defines a conserved motif important for structural integrity of the receptor and recruitment of Disheveled Cell. Signal., 2017-038(0):85-96.
PMID: 28668722
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Ma M et al. (2014-04-22), Wnt signaling in form deprivation myopia of the mice retina. PLoS ONE, 2014-09(4):e91086.
PMID: 24755605

Recombinant Mouse Norrin Protein

Catalog # 3497-NR | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Mouse Norrin Protein

Formulation, Preparation and Storage

Carrier Free

3497-NR

Carrier

3497-NR/CF

Carrier Free

Reconstitution Calculator

Background: Norrin

Citations for Recombinant Mouse Norrin Protein (2)

Product Documents for Recombinant Mouse Norrin Protein

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