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Recombinant Mouse Nope/IGDCC4 Fc Chimera Protein, CF

Catalog # 1394-NP | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
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Key Product Details

Accession #



Structure / Form

Disulfide-linked homodimer




Binding Activity

Product Specifications


Mouse myeloma cell line, NS0-derived mouse Nope/IGDCC4 protein
Mouse Nope/IGDCC4
(Gly22-His 953)
Accession # Q9EQS9
N-terminus C-terminus


>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis


Predicted Molecular Mass

128.2 kDa (monomer)


160-170 kDa, reducing conditions


Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Mouse (rm) Nope/IGDCC4 Fc Chimera at 10 µg/mL (100 µL/well) can bind rmNetrin-4 with a linear range of 8-250 ng/mL.

Formulation, Preparation and Storage

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nope/IGDCC4

Mouse Nope (Neighbor Of Punc E11) was discovered as a gene proximal to the Punc gene on chromosome 9 (1). Punc and Nope are distant members of a subgroup of the immunoglobulin (Ig) superfamily, which include DCC (Deleted in Colorectal Cancer) (2), Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues (rat UNC5H1 and H2, mouse UNC5H2 and H3, and human UNC5H3 and H4) (3), Drosophila Frazzled (4), vertebrate Neogenin (5), and mouse Nope and mouse Punc. Members of this subgroup of the Ig superfamily are type I transmembrane proteins with four Ig domains in their extracellular regions.

Mouse Nope consists of a 21 amino acid (aa) signal peptide, a 933 aa extracellular domain (including four Ig domains, five fibronectin-type III (FnIII) repeats), a 24 aa transmembrane segment, and a 274 aa cytoplasmic domain (1). The extracellular domain of mNope shares 45% aa sequence similarity with mouse Punc. However, the cytoplasmic domains of mNope and mouse Punc do not share aa sequence similarity. Compared to other members of the subgroup of the Ig superfamily, mouse Nope extracellular domain shares 25.8% and 25.2% aa similarity with mouse DCC and mouse Neogenin, respectively. Mouse and human Nope share 90.8% aa sequence similarity. Mouse Nope is expressed mostly in embryonic muscle tissues and in developing and adult nervous systems. The structural similarities between Nope and the guidance receptor of the DCC family suggest that Nope may have similar functions as the DCC family (6 - 8).


  1. Salbaum, J.M. and C. Kappen (2000) Genomics 64:15.
  2. Fearon et al. (1990) Science 247:49.
  3. Keino-Masu et al. (1996) Cell 87:175.
  4. Hong, K. et al. (1999) Cell 97:927.
  5. Leonardo, E.D. et al. (1997) Nature 386:833.
  6. Kolodziej et al. (1996) Cell 87:197.
  7. Meyerhardt (1997) Oncogene 14:1129.
  8. Vielmetter et al. (1997) Genomics 41:414.

Long Name

Neighbor of Punc E11/Immunoglobulin Superfamily, DCC Subclass, Member 4

Alternate Names


Entrez Gene IDs

57722 (Human); 56741 (Mouse)

Gene Symbol



Product Documents for Recombinant Mouse Nope/IGDCC4 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Nope/IGDCC4 Fc Chimera Protein, CF

For research use only

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