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Recombinant Mouse Noggin (aa 28-232) Protein

R&D Systems, Inc. a Bio-Techne Brand

Recombinant Mouse Noggin (aa 28-232) Protein Bioactivity
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Key Product Details

Source: NS0
Accession #: P97466
Applications: Bioactivity

Citations for Recombinant Mouse Noggin (aa 28-232) Protein

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Customer Reviews for Recombinant Mouse Noggin (aa 28-232) Protein (2)

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  • Recombinant Mouse Noggin (aa 28-232) Protein
    Name: Anonymous
    Application: Immunostaining with pSmad 1/5/8 after treatment with BMP2 or BMP2 + Noggin
    Verified Customer | Posted 10/26/2018
    Recombinant Mouse Noggin (aa 28-232) Protein Other 6997-NG
  • Recombinant Mouse Noggin (aa 28-232) Protein
    Name: Christine Quach
    Application: 3D Cell Culture
    Verified Customer | Posted 09/08/2017

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FAQs for Recombinant Mouse Noggin (aa 28-232) Protein

  • The vial is supposed to contain lyophilized protein but it appears to be empty. Is there anything in it?

    Pellets can be dislodged during shipping and become disbursed on the vial wall and in the cap. Centrifuge or tap the vial on the benchtop to return this material to the vial bottom. If this does not reveal a pellet, closely inspect the cone of the vial. Some pellets appear as only a tiny amount of material or as a transparent film due to the original buffer formulation. This is a normal appearance for many proteins. For example, if the product is originally lyophilized from a solvent such as acetonitrile or ethanol, and supplied carrier-free, you may not be able to detect the pellet with the naked eye. This does not mean the vial is empty. Reconstitute the vial as directed. After reconstitution, protein concentration can be tested with a spectrophotometer.

  • What is the recommended method for reconstitution of a lyophilized protein or antibody?

    Unless more specific directions are on the Certificate of Analysis provided with the product, we suggest the following procedure to ensure optimal recovery: 1. Allow the vial and reconstitution buffer to equilibrate to room temperature. 2. Briefly centrifuge the vial to ensure that all lyophiliate is collected at the bottom of the vial. 3. Add the amount of buffer required to achieve the concentration recommended on the product insert. 4. Allow the vial to reconstitute for 15-30 minutes at room temperature with gentle agitation, like on a rocker platform or rotating by hand.  Avoid vigorous shaking that can cause foaming and protein denaturation. 5. Aliquot into volumes greater than 20 μL and store as indicated on the product insert. If the vial exhibits flakes or particulates, mix the product for a couple of hours at room temperature and then at 4oC overnight. Contact Technical Service if product does not go into solution.   

  • Are R&D Systems recombinant proteins and antibodies sterile?

    Although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Product Documents for Recombinant Mouse Noggin (aa 28-232) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.