Mouse myeloma cell line, NS0-derived mouse Mrc2 protein Ala31-Ala1413, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
130-150 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Mrc2 His-tag (Catalog # 10491-EN) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Pro-Collagen I alpha 1 (Catalog # 6220-CL) binds with an ED50 of 0.03-0.36 μg/mL.
Recombinant Mouse Mrc2 His-tag Protein, CF Scientific Data Examples
Recombinant Mouse Mrc2 His-tag Protein Binding Activity
When Recombinant Mouse Mrc2 His-tag (10491-EN) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Pro-Collagen I alpha 1 (6220-CL) binds with an ED50 of 0.03-0.36 μg/mL.
Recombinant Mouse Mrc2 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Mouse Mrc2 His-tag (10491-EN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 145-165 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Mrc2 (C-type Mannose Receptor 2), also known as MMR2, Endocytic Receptor 180 and uPARAP, is a 180‑kDa type I transmembrane protein. It is one of the mannose receptor (MR) family members which share a common domain organization and have a broad range of biological functions (1). Mrc2 is an endocytic receptor that is found on migrating cells, including cancer cells, macrophages, fibroblasts and endothelial cells (2). Mature mouse Mrc2 is composed of 1449 amino acid (aa) that includes a 1383 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 45 aa cytoplasmic domain. The ECD shows one ricin B‑type lectin domain, one fibronectin type II domain and eight C‑type lectin domains. Within the ECD, mouse Mrc2 shares 91% and 97% aa identity with human and rat Mrc2, respectively. Mrc2 plays an important role in extracellular matrix remodeling through interaction with its ligands, including Man, Fuc, NAcGlc, collagens and urokinase plasminogen activator receptor (uPAR) (1-3). This cell surface molecule has been reported to promote cell invasion through matrix remodeling by internalizing large fragments of collagen and routing it to the lysosome for intracellular degradation and cell chemotaxis (2). It has also been reported to interact with matrix metalloprotease-13 (MMP-13) and collagen V on the cell surface (4).
Yuan, C. et al. (2016) Biochem. J. 473:2359.
Durrel, T. et al. (2018) Nat. Commun. 9:5178.
Behrendt, N. et al. (2000) J. Biol. Chem. 275:1993.
Englehom, L.H. et al. (2001) Lab. Invest.. 81:1403.
Mannose Receptor, C-type 2
Entrez Gene IDs
9902 (Human); 17534 (Mouse); 498011 (Rat)
CD280, CD280 antigen, CD280C-type mannose receptor 2, CLEC13E, CLEC13EUPAR-associated protein, ENDO180C-type lectin domain family 13 member E, Endo180, Endocytic receptor 180, KIAA0709FLJ35911, Macrophage mannose receptor 2, Mrc2, UPARAPendocytic receptor (macrophage mannose receptor family), Urokinase receptor-associated protein, Urokinase-type plasminogen activator receptor-associated protein, mannose receptor, C type 2, uPARAP, urokinase plasminogen activator receptor-associated protein
Citations for Recombinant Mouse Mrc2 His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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