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Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10516-MS

N-Terminal/MPF
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10516-MS-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Mesothelin protein
Gln36-Leu288, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln36 inferred from enzymatic pyroglutamate treatment revealing Thr37

Predicted Molecular Mass

29 kDa

SDS-PAGE

29-45 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Osteopontin/OPN  (Catalog # 441-OP) is immobilizedat 0.5 µg/mL,100  μL/well, Recombinant Mouse Mesothelin N‑Terminal (aa 36-288) (Catalog # 10516-MS) binds with an ED50 of 2-12 ng/mL.

Scientific Data Images for Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein, CF

Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein Binding Activity

Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein Binding Activity

When Recombinant Mouse Osteopontin/OPN Protein (441-OP) is immobilized at 0.5 μg/mL, 100 μL/well, it binds Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein (10516-MS) with an ED50 of 2-12 ng/mL.
Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein SDS-PAGE

Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein (Catalog # 10516-MS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 29-45 kDa.

Formulation, Preparation and Storage

10516-MS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mesothelin

Mesothelin N-terminal, commonly known as Megakaryocyte Potentiating Factor (MPF), is a 31 kDa soluble protein that was originally identified in an PHC-Y5 culture system where it exhibited megakaryocyte potentiating activity (1). MPF is closely related to mesothelin (MSLN), a 40 kDa glycosylphosphatidyl inositol (GPI)-linked protein that is highly overexpressed in various types of cancer, such as mesothelioma, ovarian and pancreatic cancers (2). Both MPF and MSLN originate from a common 71 kDa precursor protein that harbors a furin cleavage site between amino acid residues 288-293. The 31 kDa soluble N-terminal MPF fragment is secreted into blood and the C-terminal 40 kDa fragment remains membrane-bound and classified as mature mesothelin (3). Mouse MPF is composed of residues 36-288 from the precursor protein and contains one N-linked glycosylation site (N93) and one phosphorylated serine (S202). Mouse MPF shares 56% and 84% sequence identity with human and rat MPF, respectively. It is expressed mainly in the heart and lungs (4) and can be upregulated in the presence of Wnt-1 (5). MSLN was shown to be capable of binding to cancer antigen 125/mucin16 (CA125/MUC16). Apart from its role as a megakaryocyte potentiating cytokine, MPF may have the potential to serve as a biomarker for detection and monitoring of malignant mesothelioma and related cancers (6).

References

  1. Yamaguchi, N. et al. (1994) J. Biol. Chem. 260: 22:805.
  2. Hassan R. et al. (2004) Clin. Cancer Res. 10:3937.
  3. Shiomi, K. et al. (2006) Cancer. Sci. 97:928.
  4. Bera T.K. Pastan I. (2000) Mol. Cell. Biol. 20:2902.
  5. Prieve M.G. Moon R.T. (2003) BMC Dev Biol. 3:2.
  6. Raiko, I. et al. (2017) Biochem. Biophy. Res. Com. 486:526.

Alternate Names

CAK1, MPF, MSLN, SMR

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Gene Symbol

MSLN

UniProt

Additional Mesothelin Products

Product Documents for Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein, CF

For research use only

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