Recombinant Mouse Mer Fc (HEK293-expressed) Protein, CF New

Name: Recombinant Mouse Mer Fc (HEK293-expressed) Protein, CF
Brand: R&D Systems
SKU: 11314-MR
Price: 428 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 11314-MR

Product Datasheet / COA / SDS

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Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	Q60805.1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse Mer Fc (HEK293-expressed) Protein, CF

Product Specifications for Recombinant Mouse Mer Fc (HEK293-expressed) Protein, CF

Source

Human embryonic kidney cell, HEK293-derived mouse Mer protein

Mouse Mer (Glu23-Phe498) Accession # Q60805.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Glu23

Predicted Molecular Mass

79 kDa

SDS-PAGE

114-131 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Mer Fc Chimera (HEK293-expressed) (Catalog # 11314-MR) is immobilized at 2.00 µg/mL (100 µL/well), Recombinant Mouse Gas6 (Full Length) Protein (Catalog # 8310-GS) binds with an ED₅₀ of 0.400-3.60 µg/mL

Scientific Data Examples for Recombinant Mouse Mer Fc (HEK293-expressed) Protein, CF

	Recombinant Mouse Mer Fc Chimera (HEK293-expressed) Protein Binding Activity. Measured by its binding ability in a functional ELISA. When Recombinant Mouse Mer Fc Chimera (HEK293-expressed) (Catalog # 11314-MR) is immobilized at 2.00 µg/mL (100 µL/well), Recombinant Mouse Gas6 (Full Length) Protein (8310-GS) binds with an ED₅₀ of 0.400 -3.60 µg/mL
	Recombinant Mouse Mer Fc (HEK293-expressed) Protein SDS-PAGE. 2 μg/lane of Recombinant Mouse Mer Fc (HEK293-expressed) Protein (Catalog # 11314-MR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 114-131 kDa and 228-262 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11314-MR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Mer

Tyrosine-protein Kinase Mer, also known as c-Mer and MerTK, is a member of the receptor tyrosine kinase subfamily TAM (Tyro3, Axl, and Mer). Mature mouse Mer consists of 478 aa extracellular domain, 21 aa transmembrane domain, and 475 aa cytoplasmic domain. Within the extracellular domain, mouse Mer shares 77.1% and 90.4% homology with human and rat Mer, respectively. Similar to Axl and Tyro3, the extracelluar domain of Mer contains two Ig-like motifs and two fibronectin type III motifs. Mer is not expressed in normal B- and T-cells but expressed in neoplastic B- and T-cell lines (1-2). It is also show higher expression in immunosuppressive M2‑like macrophages (3). Mer is known to bind Gas6, Protein S, Tubby, Tubby-like protein 1 (Tulp1), and Galectin-3 (4-7). Upon binding ligands via the Ig-like motif, Mer is dimerized to trans-autophosphorylate the kinase domain to induce downstream signaling. It has been shown that Mer signaling in macrophages induces M2 polarization, which promote tumor growth, metastasis and evasion of anti-tumor immunity in tumor microenviroment (8). Inhibition of Mer, especially on leukocytes and macrophages, is an effective anti-cancer therapy (9).

Item	Value
References	Graham, D.K. et al. (1994) Cell Growth Differ. 5:647. Graham, D.K. et al. (2006) Clin. Cancer Res. 12:2662. Shibata, T. et al. (2014) J. Immunol. 192:3569. Nagata, K. et al. (1996) J. Biol. Chem. 271:30022. Uehara, H. et al. (2008) J. Immunol. 180:2522. Caberoy, N.B. et al. (2010) EMBO J. 29:3898. Caberoy, N.B. et al. (2012) J. Cell Physiol. 227:401. Kim, S.Y. et al. (2016) Sci. Rep. 6:29673. Cummings, C.T. et al. (2013) Clin. Cancer Res. 19:5275.
Long Name	Receptor Tyrosine Protein Kinase Mer
Entrez Gene IDs	10461 (Human); 17289 (Mouse); 102143798 (Cynomolgus Monkey)
Alternate Names	C-mer, EC 2.7.10, EC 2.7.10.1, MER receptor tyrosine kinase, MGC133349, Mer, MerTK, RP38Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK, STK kinase, c-Eyk, c-mer proto-oncogene tyrosine kinase, tyrosine-protein kinase Mer

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Recombinant Mouse Mer Fc (HEK293-expressed) Protein, CF New

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Mouse Mer Fc (HEK293-expressed) Protein, CF

Scientific Data Examples for Recombinant Mouse Mer Fc (HEK293-expressed) Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Mer

Citations for Recombinant Mouse Mer Fc (HEK293-expressed) Protein, CF

Product Documents for Recombinant Mouse Mer Fc (HEK293-expressed) Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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