Recombinant Mouse LAIR1 His-tag Protein, CF

Name: Recombinant Mouse LAIR1 His-tag Protein, CF
Brand: R&D Systems
SKU: 4016-LR
Rating: 4 (2 reviews)

Catalog # 4016-LR | R&D Systems, Inc. a Bio-Techne Brand

(2)

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Key Product Details

Source:	NS0
Accession #:	Q8BG84
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse LAIR1 His-tag Protein, CF

Source	Mouse myeloma cell line, NS0-derived mouse LAIR1 protein Gln22-Tyr141 & Ser25-Tyr141, both with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser25 & No results obtained: Gln22 predicted
Predicted Molecular Mass	14.3 kDa
SDS-PAGE	20-35 kDa, reducing conditions
Activity	Measured by the ability of the immobilized protein to support the adhesion of HT‑29 human colon adenocarcinoma cells. When 5 x 10⁴ cells/well are added to Recombinant Mouse LAIR1-coated plates (50 µg/mL with 100 µL/well), approximately 30‑60% will adhere after 10 minutes at 37° C. Optimal concentration depends on cell type as well as the application or research objectives.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4016-LR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

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Background: LAIR1

Leukocyte-associated Ig-like receptor-1 (LAIR1) is an inhibitory receptor of the Ig superfamily that is structurally related to inhibitory members of KIR and ILT/CD85 families (1-3). It is expressed on immune cells, including NK cells, T cells, B cells, monocytes, immature neutrophils, dendritic cells and most thymocytes (2-4). The 253 amino acid (aa) type I transmembrane (TM) protein contains a 21 aa signal sequence, a 124 aa extracellular domain (ECD), a 20 aa TM domain and a 98 aa cytoplasmic domain. The ECD includes one C2-type Ig-like domain and two potential N-linked glycosylation sites. Tyrosine phosphorylation of two cytoplasmic ITIM motifs results in recruitment of phosphatases and down-regulation of signaling through activating receptors (2, 3, 5). Crosslinking of LAIR1 inhibits processes such as B cell receptor-mediated activation, NK cell and T cell cytotoxicity and basophil degranulation (1-3). Four mouse LAIR1 splice variants have been identified, but it is not known whether they are expressed as proteins (3). LAIR1b, which is the major alternate transcript, lacks most of the ECD. Of the minor transcripts, LAIR1c lacks a signal sequence, and LAIR1d and 1e lack a TM sequence. All mouse splice forms are identical in the last 90 aa of the cytoplasmic domain. LAIR1 shows high-affinity binding of collagens that results in inhibition of degranulation in a basophilic leukemia cell line (6). Human and mouse LAIR1 ECD share only 32% aa identity but, where studied, sites of expression and activity are similar (3-6). Mouse LAIR1 ECD also shares 62%, 31% and 28% aa identity with rat, canine, and bovine orthologs, respectively.

Item	Value
References	Meyaard, L. (2003) J. Biol. Regul. Homeost. Agents 17:330. Meyaard, L. et al. (1997) Immunity 7:283. Lebbink, R.J. et al. (2004) J. Immunol. 172:5535. Verbrugge, A. et al. (2006) J. Leukoc. Biol. 79:828. Verbrugge, A. et al. (2003) Int. Immunol. 15:1349. Lebbink, R.J. et al. (2006) J. Exp. Med. 203:1419.
Long Name	Leukocyte-associated Ig-like Receptor 1
Entrez Gene IDs	3903 (Human); 52855 (Mouse); 574531 (Rat); 102136964 (Cynomolgus Monkey)
Alternate Names	CD305, CD305 antigen, CD305leukocyte-associated Ig-like receptor 1, HLAIR1, LAIR-1, LAIR1, leukocyte-associated immunoglobulin-like receptor 1

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Customer Reviews for Recombinant Mouse LAIR1 His-tag Protein, CF (2)

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Application: Binding assay/Protein-protein interaction

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Application: Binding assay/Protein-protein interaction

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Product Documents for Recombinant Mouse LAIR1 His-tag Protein, CF

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Recombinant Mouse LAIR1 His-tag Protein, CF

Catalog # 4016-LR | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Mouse LAIR1 His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: LAIR1

Citations for Recombinant Mouse LAIR1 His-tag Protein, CF

Customer Reviews for Recombinant Mouse LAIR1 His-tag Protein, CF (2)

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Recombinant Mouse LAIR1 His-tag Protein, CF

Recombinant Mouse LAIR1 His-tag Protein, CF

Product Documents for Recombinant Mouse LAIR1 His-tag Protein, CF

Product Datasheets

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