Recombinant Mouse Klotho (aa 35-982) Protein, CF

Name: Recombinant Mouse Klotho (aa 35-982) Protein, CF
Brand: R&D Systems
SKU: 1819-KL
Rating: 4 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1819-KL

Citations (24)

(3)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	CHO
Accession #:	O35082.2
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse Klotho (aa 35-982) Protein, CF

Product Specifications for Recombinant Mouse Klotho (aa 35-982) Protein, CF

Source	Chinese Hamster Ovary cell line, CHO-derived mouse Klotho protein Ala35-Lys982, with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala35
Predicted Molecular Mass	109.5 kDa
SDS-PAGE	125-130 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human FGF RIIIc. The ED₅₀ for this effect is 0.04-0.4 µg/mL in the presence of 1 µg/mL of Recombinant Human FGF‑23 (Catalog # 2604-FG) and 10 µg/mL of heparin.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1819-KL

Formulation	Supplied as a 0.2 μm filtered solution in PBS, Glycerol and EDTA.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Klotho

Klotho, also called Klotho-alpha, is the founding member of the Klotho family within the glycosidase-1 superfamily (1, 2). Klotho is expressed in areas concerned with calcium regulation, predominantly in the kidney distal convoluted tubules, but also in the brain choroid plexus (which produces cerebrospinal fluid) and the parathyroid (1). The 1014 amino acid (aa) type I transmembrane protein contains a 34 aa signal sequence, a 948 aa extracellular domain (ECD) containing two extracellular glycosidase-like domains, a 21 aa transmembrane domain and an 11 aa intracellular domain. Within the ECD, mouse Klotho shares 95%, 87% and 87% aa identity with rat, human and equine Klotho, respectively. Although a truncated 554 aa isoform predicts a soluble 70 kDa form, the soluble form found in plasma and cerebrospinal fluid is a 130 kDa form produced by proteolytic cleavage of the glycosylated 135 kDa full-length Klotho (3, 4). A prominent intracellular 120 kDa form of Klotho is localized to endoplasmic reticulum and Golgi membranes (4). Klotho is named for the Greek goddess who spins the thread of life. The phenotype of Klotho-deficient mice resembles premature aging, including arteriosclerosis, osteoporosis, skin atrophy, infertility, emphysema and premature death (2). Conversely, excess Klotho extends lifespan (5). Klotho acts as a cofactor for interaction of FGF23 with FGF R1 (6). This interaction negatively regulates 1 alpha -hydroxylase, the rate-limiting enzyme in the synthesis of 1,25(OH)₂D₃ (vitamin D) (7). Klotho-deficient mice show severe hyperphosphatemia and ectopic calcification of soft tissues due to excess vitamin D (2, 7). Both Klotho and Klotho-beta are cofactors for FGF19 binding (8). Klotho also shows glucuronidase activity which activates the renal ion channel TRPV5 to reabsorb urinary calcium (9). Klotho has been reported to downregulate insulin or IGF-1 signaling in adipocytes, to bind and antagonize Wnt molecules, and to facilitate release of parathyroid hormone (10 - 12).

Item	Value
References	Nabeshima, Y. (2006) Sci. Aging Knowl. Environ. 8:pe11. Kuro-o, M. et al. (1997) Nature 390:45. Shiraki-Iida, T. et al. (1998) FEBS Lett. 424:6. Imura, A. et al. (2004) FEBS Lett. 565:143. Kurosu, H. et al. (2005) Science 309:1829. Kurosu, H. et al. (2006) J. Biol. Chem. 281:6120. Tsujikawa, H. et al. (2003) Mol. Endocrinol. 17:2393. Wu, X. et al. (2007) J. Biol. Chem. 282:29069. Chang, Q. et al. (2005) Science 310:490. Yamamoto, M. et al. (2005) J. Biol. Chem. 280:38029. Liu, H. et al. (2007) Science 317:803. Imura, A. et al. (2007) Science 316:1615.
Entrez Gene IDs	9365 (Human); 16591 (Mouse)
Alternate Names	EC 3.2.1, EC 3.2.1.31, KL, Klotho

Citations for Recombinant Mouse Klotho (aa 35-982) Protein, CF (24)

Showing 1 - 10 of 24 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Klotho (aa 35-982) Protein, CF include:

Species: Mouse
Sample Types: In Vivo
Applications: Bioassay

C Du et al. (2022), Renal Klotho and inorganic phosphate are extrinsic factors that antagonistically regulate hematopoietic stem cell maintenance Cell Reports, 38(7):110392.
PMID: 35172146
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Y Wu et al. (2022), Phosphate Metabolic Inhibition Contributes to Irradiation-Induced Myelosuppression through Dampening Hematopoietic Stem Cell Survival Nutrients, 14(16).
PMID: 36014901
Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture

S Shrestha et al. (2022), Angiotensin II receptor type 1 blockade regulates Klotho expression to induce TSC2-deficient cell death The Journal of Biological Chemistry, 0(0):102580.
PMID: 36220392
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

VW Nakao et al. (2022), Neuroprotective action of alpha-Klotho against LPS-activated glia conditioned medium in primary neuronal culture Scientific Reports, 12(1):18884.
PMID: 36344527
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay

X Zhuang et al. (2021), Klotho attenuated Doxorubicin-induced cardiomyopathy by alleviating Dynamin-related protein 1 - mediated mitochondrial dysfunction Mechanisms of Ageing and Development, 195(0):111442.
PMID: 33539906
Species: Rat
Sample Types: cardiomyocyte
Applications: Bioassay

WY Chen (2020), Soluble Alpha-Klotho Alleviates Cardiac Fibrosis without Altering Cardiomyocytes Renewal Int J Mol Sci, 21(6).
PMID: 32235720
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

S Batlahally et al. (2020), Soluble Klotho, a biomarker and therapeutic strategy to reduce bronchopulmonary dysplasia and pulmonary hypertension in preterm infants Sci Rep, 10(1):12368.
PMID: 32704023
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

T Landry et al. (2020), Centrally circulating &alpha-klotho inversely correlates with human obesity and modulates arcuate cell populations in mice Molecular Metabolism, 44(0):101136.
PMID: 33301986
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Z Zhang et al. (2020), Klotho Alleviates Lung Injury Caused by Paraquat via Suppressing ROS/P38 MAPK-Regulated Inflammatory Responses and Apoptosis Oxid Med Cell Longev, 2020(0):1854206.
PMID: 32509139
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Z Rao et al. (2019), Administration of alpha klotho reduces liver and adipose lipid accumulation in obese mice Heliyon, 5(4):e01494.
PMID: 31049427
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Y Qian et al. (2018), Klotho Reduces Necroptosis by Targeting Oxidative Stress Involved in Renal Ischemic-Reperfusion Injury Cell. Physiol. Biochem., 45(6):2268-2282.
PMID: 29550818
T Arbel Rubi et al. (2018), Klotho suppresses colorectal cancer through modulation of the unfolded protein response Oncogene, 0(0).
PMID: 30232408
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

L Zhu et al. (2018), Klotho controls the brain-immune system interface in the choroid plexus Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 30413620
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

CH Mazucanti et al. (2018), Activity-dependent neuronal Klotho enhances astrocytic aerobic glycolysis J. Cereb. Blood Flow Metab., 0(0):271678X187627.
PMID: 29493420
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

T Minamizaki et al. (2018), Soluble Klotho causes hypomineralization in Klotho-deficient mice J. Endocrinol., 0(0).
PMID: 29632215
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

R Behera et al. (2017), Inhibition of age-related therapy resistance in melanoma by rosiglitazone-mediated induction of Klotho Clin. Cancer Res, 0(0).
PMID: 28232477
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

J Leon et al. (2017), Peripheral Elevation of a Klotho Fragment Enhances Brain Function and Resilience in Young, Aging, and ?-Synuclein Transgenic Mice Cell Rep, 20(6):1360-1371.
PMID: 28793260
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

SW Lim et al. (2017), Klotho enhances FoxO3-mediated manganese superoxide dismutase expression by negatively regulating PI3K/AKT pathway during tacrolimus-induced oxidative stress Cell Death Dis, 8(8):e2972.
PMID: 28771227
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

F Salech et al. (2017), Local Klotho enhances neuronal progenitor proliferation in the adult hippocampus J. Gerontol. A Biol. Sci. Med. Sci., 0(0).
PMID: 29300914
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Vadakke Madathil S et al. (2014), Klotho deficiency disrupts hematopoietic stem cell development and erythropoiesis. Am J Pathol, 184(3):827-41.
PMID: 24412515
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

Andrukhova O et al. (2014), FGF23 promotes renal calcium reabsorption through the TRPV5 channel. EMBO J, 33(3):229-46.
PMID: 24434184
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Kawai M et al. (2013), FGF23 suppresses chondrocyte proliferation in the presence of soluble alpha-Klotho both in vitro and in vivo. J Biol Chem, 288(4):2414-27.
PMID: 23235154
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Song et al. (2013), Klotho suppresses cardiomyocyte apoptosis in mice with stress-induced cardiac injury via downregulation of endoplasmic reticulum stress. PLoS ONE, 8(12):e82968.
PMID: 24340070
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Lau et al. (2012), Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet. Kidney Int, 82(12):1261-70.
PMID: 22932118

There are no citations that match your criteria.

Showing 1 - 10 of 24 citations Showing All

Customer Reviews for Recombinant Mouse Klotho (aa 35-982) Protein, CF (3)

4 out of 5

3 customer ratings

5 stars

4 stars

100%

3 stars

2 stars

1 star

Customer Images

Showing 1 - 3 of 3 reviews Showing All

Filter By:

Recombinant Mouse Klotho (aa 35-982) Protein, CF

Name: Anonymous

Application: In vivo study

Verified Customer | Posted 11/16/2022
Recombinant Mouse Klotho (aa 35-982) Protein, CF

Name: Anonymous

Application: In vivo study

Verified Customer | Posted 11/02/2022

In vivo experimental model as a treatment against kidney injury
Name: Anonymous

Application: In vivo study

Reason for Rating: Treatment with this product reduced my experimental model injury

Verified Customer | Posted 12/12/2018

There are no reviews that match your criteria.

Showing 1 - 3 of 3 reviews Showing All

Product Documents for Recombinant Mouse Klotho (aa 35-982) Protein, CF

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Recombinant Mouse Klotho (aa 35-982) Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Product Specifications for Recombinant Mouse Klotho (aa 35-982) Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Klotho

Citations for Recombinant Mouse Klotho (aa 35-982) Protein, CF (24)

Customer Reviews for Recombinant Mouse Klotho (aa 35-982) Protein, CF (3)

Customer Images

Recombinant Mouse Klotho (aa 35-982) Protein, CF

Recombinant Mouse Klotho (aa 35-982) Protein, CF

Product Documents for Recombinant Mouse Klotho (aa 35-982) Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected