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Recombinant Mouse Kell Protein, CF

Catalog # 1454-ZN | R&D Systems, Inc. a Bio-Techne Brand
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1454-ZN-010

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Kell protein
Phe50-Trp713, with an N-terminal 7-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

76 kDa

SDS-PAGE

106 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005).
The specific activity is >0.2 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

1454-ZN
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and ZnCl2.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Kell

Kell, a type II membrane glycoprotein, is linked through a single disulfide bond to XK, a putative membrane transporter. The two proteins constitute the Kell blood group antigens (1). Kell is a zinc endopeptidase of the neprilysin (NEP) family which also includes endothelin converting enzymes (ECE-1 and ECE-2), PEX, XCE, DINE and several NEP-like proteins (2). It has been shown to cleave big endothelin-3 (ET-3) at Trp21-Ile22, yielding ET-3, and to a much lesser extent, big ET-1 and big ET-2 at Trp21-Val22, yielding ET-1 and ET-2 (3). Several different molecular defects cause the Kell null phenotype, which has no obvious clinical outcome (4).

References

  1. Lee, S. et al. (2000) Transfus. Med. Rev. 14:93.
  2. Turner, A.J. et al. (2001) BioEssays. 23:261.
  3. Lee, S. et al. (1999) Blood 94:1440.
  4. Lee, S. et al. (2001) J. Biol. Chem. 276:27281.

Long Name

Zinc protease blood group antigen

Alternate Names

CD238, KEL

Entrez Gene IDs

3792 (Human); 23925 (Mouse)

Gene Symbol

KEL

UniProt

Additional Kell Products

Product Documents for Recombinant Mouse Kell Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Kell Protein, CF

For research use only

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