What is the amino acid sequence of the acidic and basic tails?

Name: Recombinant Mouse Integrin alpha V beta 3 Protein, CF
Brand: R&D Systems
SKU: 7889-AV

Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

Recombinant Mouse Integrin alpha V beta 3 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 7889-AV

Citations (3)

Product Datasheet / COA / SDS

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Key Product Details

Source:	CHO
Accession #:	P43406 (Integrin alpha V) & O54890 (Integrin beta 3)
Structure / Form:	Noncovalently-linked heterodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse Integrin alpha V beta 3 Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha V beta 3 protein

Mouse Integrin alpha V (Phe31-Val988) Accession # P43406	His-Pro	GGGSGGGS	Acidic Tail	6-His tag
Mouse Integrin beta 3 (Glu26-Asp717) Accession # O54890	His-Pro	GGGSGGGS	Basic Tail
N-terminus				C-terminus

Purity

>90%, by SDS-PAGE with silver staining

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Phe31 (Integrin alpha V) & Glu26 (Integrin beta 3)

Predicted Molecular Mass

115 kDa (Integrin alpha V) & 84.5 kDa (Integrin beta 3)

SDS-PAGE

135-145 kDa & 100-115 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Vitronectin (Catalog # 2308-VN) at 1 µg/mL can bind Recombinant Mouse Integrin alpha V beta 3 with an apparent K_D <5 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7889-AV

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 400 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha V beta 3

Integrin alpha V beta 3 together with alpha IIb beta ₃, constitutes the only known beta 3 Integrins (1‑3). The non‑covalent heterodimer of 170 kDa alpha V/CD51 and 93 kDa beta ₃/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2‑4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alpha V beta 3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alpha V beta 3 is in the N‑terminal head region, formed by interaction of the beta 3 vWFA domain with the alpha V beta-propeller structure (4). The alpha V subunit contributes a thigh and a calf region, while the beta 3 subunit contains a PSI domain and four cysteine‑rich I‑EGF folds. The alpha V subunit domains termed thigh, calf‑1 and calf‑2 generate a “knee” region that is bent when the alpha V beta 3 is in its constitutively inactive state. Activation, either by “inside out” signaling or by Mg²⁺ or Mn²⁺ binding, extends the Integrin to expose its ligand binding site (1, 4). The 958 aa mouse alpha V ECD shares 92‑95% aa sequence identity with human, rat and bovine alpha V while the 692 aa mouse beta ₃ ECD shares 95% aa identity with rat and 89‑91% with human, canine, equine, bovine and porcine beta ₃. alpha V beta 3 is essential for the maturation of osteoclasts and their binding and resorption of bone; it also, however, promotes their apoptosis (5, 6). M‑CSF R and alpha V beta 3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (5, 6). alpha V beta 3 is involved in several other signaling pathways by direct interaction with receptor tyrosine kinases and ligands. For example, it cooperates with endothelial cell VEGF R2 in angiogenesis, and with IGF‑1 to promote cancer cell proliferation and invasiveness (7, 8). Also, cell entry of several viruses is mediated by alpha V beta 3 (4, 9).

Item	Value
References	Hynes, R. O. (2002) Cell 110:673. Serini, G. et al. (2006) Exp. Cell Res. 312:651. Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88. Xiong, J. et al. (2001) Science 294:339. McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433. Faccio, R. et al. (2003) J. Clin. Invest. 111:749. Somanath, P.R. et al. (2009) Angiogenesis 12:177. Saegusa, J. et al. (2009) J. Biol. Chem. 284:24106. Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533.
Entrez Gene IDs	3685 (Human)
Alternate Names	CD51, CD51 antigen, Integrin alpha V beta 3, MSK8, VNRADKFZp686A08142, Vitronectin receptor subunit alpha, antigen identified by monoclonal L230, integrin alpha-V, integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)

Citations for Recombinant Mouse Integrin alpha V beta 3 Protein, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Integrin alpha V beta 3 Protein, CF include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

M Bieri et al. (2021), The RGD-binding integrins alphavbeta6 and alphavbeta8 are receptors for mouse adenovirus-1 and -3 infection PloS Pathogens, 17(12):e1010083.
PMID: 34910784
Species: Mouse
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR

KH Koh et al. (2019), Nonimmune cell-derived ICOS ligand functions as a renoprotective alphavbeta3 integrin-selective antagonist J. Clin. Invest., 129(4):1713-1726.
PMID: 30747722
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Y Negishi et al. (2018), Development of a Screening System for Targeting Carriers Using Peptide-Modified Liposomes and Tissue Sections Biol. Pharm. Bull., 41(7):1107-1111.
PMID: 29962407

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FAQs for Recombinant Mouse Integrin alpha V beta 3 Protein, CF

What is the amino acid sequence of the acidic and basic tails?

Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

Recombinant Mouse Integrin alpha V beta 3 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Mouse Integrin alpha V beta 3 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Integrin alpha V beta 3

Citations for Recombinant Mouse Integrin alpha V beta 3 Protein, CF (3)

FAQs for Recombinant Mouse Integrin alpha V beta 3 Protein, CF

Product Documents for Recombinant Mouse Integrin alpha V beta 3 Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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