Recombinant Mouse IL-3R alpha/CD123 Fc Chimera Protein, CF

Name: Recombinant Mouse IL-3R alpha/CD123 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 983-MR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 983-MR

Citations (1)

Product Datasheet / COA / SDS

Catalog #

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Key Product Details

Source:	Sf 21 (baculovirus)
Accession #:	P26952
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse IL-3R alpha/CD123 Fc Chimera Protein, CF

Product Specifications for Recombinant Mouse IL-3R alpha/CD123 Fc Chimera Protein, CF

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse IL-3 R alpha/CD123 protein

Mouse IL-3 R alpha (Ser17-Lys331) Accession # P26952	IEGRID	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ser17

Predicted Molecular Mass

61.0 kDa (monomer)

SDS-PAGE

70-80 kDa, reducing conditions

Activity

Measured by its ability to inhibit the IL-3-induced proliferation of NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 0.03‑0.15 µg/mL in the presence of 2.5 µg/mL Recombinant Mouse IL‑3 R beta Fc Chimera (Catalog # 549‑R3) and 2 ng/mL of Recombinant Mouse IL‑3 (Catalog # 403‑ML).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

983-MR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-3R alpha

The IL-3 receptor chain (IL‑3 R alpha ; also SUT-1 and CD123) is a 60‑70 kDa member of the class I cytokine receptor family of proteins (1, 2, 3). Members of this class are type I transmembrane proteins that contain two fibronectin type III (FNIII) modules and a distinctive Trp‑Ser‑x‑Trp‑Ser peptide motif in the extracellular domain. This is in contrast to class II receptors that share the same fibronectin domains but lack both the WSxWS motif and analogous cysteine spacings. Mouse IL‑3 R alpha is synthesized as a 396 amino acid (aa) precursor that contains a 16 aa signal sequence, a 315 aa extracellular region, a 24 aa transmembrane segment, and a 41 aa cytoplasmic tail (3, 4). The molecule is expressed on multiple cell types, including endothelial cells (5), monocytes (6), eosinophils (7), basophils plus mast cells (8), and plasmacytoid CD4⁺ T cells (9). IL‑3 R alpha serves as a low affinity binding protein for 24‑28 kDa monomeric IL‑3 (1, 3, 4, 10, 11). Following binding, the IL‑3:IL‑3 R alpha complex likely interacts with a preformed signaling homodimer comprised of either 120 kDa AIC2A (= beta _IL-3) or 130 kDa AIC2B (= beta c) chains. This tetramer appears to recruit one more IL‑3:IL‑3 R alpha complex, generating a fully functional hexamer (1, 3, 4, 12, 13). Signaling is mediated by receptor‑associated cytoplasmic kinases, as class I cytokine receptors do not possess intrinsic kinase activity. Notably, the IL‑3 R complex may not exist as a singularity, but actually form higher‑order complexes with FcR gamma, beta ₁ integrin, and VEGF R2 (1). There are at least two IL‑3 Ra isoform variants in mouse. One is found in the AKR and A/J strains, and shows a deletion of aa 274‑283. This is not present at the cell membrane (14). The second (called SP2) shows a deletion of aa 20‑112, generating a 50‑55 kDa membrane form that binds IL‑3, but will only signal through beta _IL-3 (15, 16). IL‑3’s choice of full‑length IL‑3 R alpha (SP1) or SP2 leads to different signaling outcomes, presumably due to the engagement of different binding sites on beta _IL-3. The extracellular domain of mouse IL‑3 R alpha /SP1 shares only 44% and 29% aa sequence identity with rat and human IL‑3 R alpha, respectively. Nevertheless, human IL‑3 is reported to be active on the mouse receptor complex that is expressed on select cell types (17).

Item	Value
References	Broughton, S.E. et al. (2012) Immunol. Rev. 250:277. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33. Miyajima, A. et al. (1993) Blood 82:1960. Hara, T. & A. Miyajima (1992) EMBO J. 11:1875. Korpelainen, E.I. et al. (1993) Proc. Natl. Acad. Sci. USA 90:11137. Ebner, S. et al. (2002) J. Immunol. 168:6199. Gregory, B. et al. (2003) J. Immunol. 170:5359. Dahl, C. et al. (2004) Allergy 59:1087. Grouard, G. et al. (1997) J. Exp. Med. 185:1101. Clark-Lewis, I. et al. (1984) J. Biol. Chem. 259:7488. Knepper, T.P. et al. (1992) Biochemistry 31:11651. Ogorochi, T. et al. (1992) Blood 79:895. Murphy, J.M. et al. (2004) J. Biol. Chem. 279:26500. Ichihara, M. et al. (1995) EMBO J. 14:939. Chen, J. et al. (2009) J. Biol. Chem. 284:5763. Mirza, S. et al. (2010) J. Biol. Chem. 285:22370. Dentelli, P. et al. (1999) J. Immunol. 163:2151.
Long Name	Interleukin 3 Receptor alpha
Entrez Gene IDs	3563 (Human); 16188 (Mouse); 102138639 (Cynomolgus Monkey)
Alternate Names	CD123, CD123 antigen, IL-3 R alpha, IL-3 receptor subunit alpha, IL-3R alpha, IL-3R subunit alpha, IL-3R-alpha, IL-3Ra, IL3R, IL3R alpha, IL3RA, IL3RAY, IL3RX, IL3RY, MGC34174, hIL-3Ra, hIL3Ra, interleukin 3 receptor, alpha (low affinity), interleukin-3 receptor subunit alpha

Citations for Recombinant Mouse IL-3R alpha/CD123 Fc Chimera Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse IL-3R alpha/CD123 Fc Chimera Protein, CF include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Mille F et al. (2009), Interfering with multimerization of netrin-1 receptors triggers tumor cell death. Cell Death Differ., 16(10):1344-51.
PMID: 19543238

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Recombinant Mouse IL-3R alpha/CD123 Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Mouse IL-3R alpha/CD123 Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: IL-3R alpha

Citations for Recombinant Mouse IL-3R alpha/CD123 Fc Chimera Protein, CF (1)

Product Documents for Recombinant Mouse IL-3R alpha/CD123 Fc Chimera Protein, CF

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