Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Recombinant Mouse IL-3 Protein Best Seller

Catalog # 403-ML | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
Loading...
Carrier Free
403-ML-010/CF
403-ML-01M/CF
Show More Sizes
403-ML-025/CF
403-ML-050/CF
403-ML-100/CF
403-ML-500/CF
Show Fewer Sizes

Catalog #
Availability
Size / Price
Qty
With Carrier
403-ML-010
403-ML-025
Show More Sizes
403-ML-050
403-ML-100
403-ML-500
Show Fewer Sizes

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived mouse IL-3 protein
Asp33-Cys166, with an N-terminal Met

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp33

Predicted Molecular Mass

15 kDa

Activity

Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. Holmes, K.L. et al. (1985) Proc. Natl. Acad. Sci. USA 82:6687.
The ED50 for this effect is 20-100 pg/mL.

Scientific Data Images for Recombinant Mouse IL-3 Protein

Recombinant Mouse IL‑3 Protein SEC-MALS.

Recombinant Mouse IL-3 Protein (Catalog # 403-ML) has a molecular weight (MW) of 14.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.
Recombinant Mouse IL-3 Protein Bioactivity

Recombinant Mouse IL-3 Protein Bioactivity

Recombinant Mouse IL-3 (Catalog # 403-ML) stimulates cell proliferation of the NFS-60 mouse myelogenous leukemia lymphoblast cell line. The ED50 for this effect is 20-100 pg/mL.
Recombinant Mouse IL-3 Protein SDS-PAGE

Recombinant Mouse IL-3 Protein SDS-PAGE

1 μg/lane of Recombinant Mouse IL-3 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 15 kDa.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 403-ML
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 403-ML/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-3

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated mouse mast cells, mouse keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and mouse IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on mouse cells.

IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of alpha and beta subunits. The IL-3R alpha is a member of the cytokine receptor super family and binds IL-3 with low affinity. Two distinct beta subunits, AIC2A ( betaIL-3) and AIC2B ( betac) are present in mouse cells. betaIL-3 also binds IL-3 with low affinity and forms a high affinity receptor with the alpha subunit. The betac subunits does not bind any cytokine but forms functional high affinity receptors with the alpha subunit of the IL-3, IL-5 and GM-CSF receptors. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.

References

  1. Yokota, T. et al., 1984, Proc. Natl. Acad. Sci. USA 81:1070.
  2. Fung, M.C. et al., 1984, Nature 307:233.
  3. Miyatake, S. et al., 1985, Proc. Natl. Acad. Sci. USA 82:316.

Long Name

Interleukin 3

Alternate Names

IL3, MCGF, MULTI-CSF

Entrez Gene IDs

3562 (Human); 16187 (Mouse); 24495 (Rat)

Gene Symbol

IL3

UniProt

Additional IL-3 Products

Product Documents for Recombinant Mouse IL-3 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse IL-3 Protein

For research use only

Citations for Recombinant Mouse IL-3 Protein (171)

Showing  1 - 10 of 171 publications Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse IL-3 Protein include:

There are no citations that match your criteria.

Showing  1 - 10 of 171 publications Showing All

Customer Reviews for Recombinant Mouse IL-3 Protein (6)

4.3 out of 5
6 customer ratings
5 stars
33%
4 stars
67%
3 stars
0%
2 stars
0%
1 star
0%
Showing  1 - 5 of 6 reviews Showing All
Filter By:
  • Recombinant Mouse IL-3 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 11/20/2021
  • Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 06/15/2018
  • Name: Anonymous
    Application: Cell Adhesion
    Verified Customer | Posted 05/14/2018
  • Name: Anonymous
    Verified Customer | Posted 04/26/2017
    I used IL-3 to culture my Ba/F3 cells. With IL3, they are growing well and viable.
  • Recombinant Mouse IL-3 Protein
    Name: Marco Ramos
    Application: Cell Proliferation
    Verified Customer | Posted 04/04/2017
  • Recombinant Mouse IL-3 Protein
    Name: Anonymous
    Application: Cell migration/motility
    Verified Customer | Posted 05/18/2016

There are no reviews that match your criteria.

Showing  1 - 5 of 6 reviews Showing All

FAQs for Recombinant Mouse IL-3 Protein

Showing  1 - 1 of 1 FAQ Showing All
  • Q: Is there a mass to unit conversion for Recombinant Mouse IL-3 Protein (Catalog # 403-ML & 403-ML/CF)?

    A: We have developed an approximate mass to unit conversion factor for Recombinant Mouse IL-3 Protein (Catalog # 403-ML & 403-ML/CF) based on testing against WHO Standard (NIBSC code: 91/662). In this testing, we found a relative conversion of 1 ug of cytokine = 1.7 x 105 Units. This information can be found in our Unit Conversion Table: https://www.rndsystems.com/resources/technical-information/unit-conversion-table Please note this information is for reference purposes only and is not routinely tested.

Showing  1 - 1 of 1 FAQ Showing All

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=
Loading...
Setting the standard in quality research reagents