Recombinant Mouse IL-17A (CHO-expressed) Protein

Name: Recombinant Mouse IL-17A (CHO-expressed) Protein
Brand: R&D Systems
SKU: 7956-ML
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 7956-ML

Citations (3)

(1)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	CHO
Accession #:	Q62386
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse IL-17A (CHO-expressed) Protein

Product Specifications for Recombinant Mouse IL-17A (CHO-expressed) Protein

Source	Chinese Hamster Ovary cell line, CHO-derived mouse IL-17/IL-17A protein Ala26-Ala158
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala26
Predicted Molecular Mass	15 kDa (monomer)
SDS-PAGE	15 -22 kDa, reducing conditions
Activity	Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.25-1.25 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

7956-ML

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7956-ML/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-17/IL-17A

Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature mouse IL‑17A shares 61% and 89% amino acid sequence identity with human and rat IL‑17A, respectively (3, 4). IL‑17A is secreted by Th17 cells, gamma /δ T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide‑linked homodimers as well as disulfide‑linked heterodimers with IL‑17F (5, 6). IL‑17A exerts its effects through the transmembrane IL‑17 RA in complex with IL‑17 RC or IL‑17 RD (7, 8). Both IL‑17 RA and IL‑17 RC are required for responsiveness to heterodimeric IL‑17A/F (7). IL‑17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9‑12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides (9‑11). IL‑17A/F likewise induces neutrophil migration, but IL‑17F does not (11). IL‑17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF‑ alpha induced shock (Fossiez, 14). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL‑17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (15, 16). IL‑17A has been shown to exert either tumorigenic or anti‑tumor effects (17, 18).

Item	Value
References	Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556. Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479. Yao, Z. et al. (1996) Gene 168:223. Rouvier, E. et al. (1993) J. Immunol. 150:5445. Chang, S.H. and C. Dong (2007) Cell Res. 17:435. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447. Wright, J.F. et al. (2008) J. Immunol. 181:2799. Rong, Z. et al. (2009) Cell Res. 19:208. Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762. Liang, S.C. et al. (2006) J. Exp. Med. 203:2271. Liang, S.C. et al. (2007) J. Immunol. 179:7791. O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593. Takahashi, N. et al. (2008) J. Exp. Med. 205:1755. Hsu, H. et al. (2008) Nat. Immunol. 9:166. Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857. Wang, L. et al. (2009) J. Exp. Med. 206:1457. Kryczek, I. et al. (2009) Blood 114:357.
Long Name	Interleukin 17
Entrez Gene IDs	3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names	CTLA-8, CTLA8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL-17, IL-17A, IL-17Acytotoxic T-lymphocyte-associated protein 8, IL-17CTLA-8, IL17, IL17A, IL17interleukin-17A, interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8), interleukin 17A

Citations for Recombinant Mouse IL-17A (CHO-expressed) Protein (3)

Showing 1 - 3 of 3 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse IL-17A (CHO-expressed) Protein include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

D Knizkova et al. (2022), CMTM4 is a subunit of the IL-17 receptor and mediates autoimmune pathology Nature Immunology, 0(0).
PMID: 36271145
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Y Wang et al. (2021), Blocking the interaction between interleukin-17A and endoplasmic reticulum stress in macrophage attenuates retinal neovascularization in oxygen-induced retinopathy Cell bioscience, 2021;11(1):82.
PMID: 33933165
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

EM Byrne et al. (2021), IL-17A Damages the Blood-Retinal Barrier through Activating the Janus Kinase 1 Pathway Biomedicines, 9(7).
PMID: 34356895

There are no citations that match your criteria.

Showing 1 - 3 of 3 citations Showing All

Customer Reviews for Recombinant Mouse IL-17A (CHO-expressed) Protein (1)

5 out of 5

1 customer rating

5 stars

100%

4 stars

3 stars

2 stars

1 star

Showing 1 - 1 of 1 review Showing All

Filter By:

Recombinant Mouse IL-17A (CHO-expressed) Protein

Name: Anonymous

Application: In vivo study

Verified Customer | Posted 06/16/2020

Recombinant Mouse IL-17A (CHO-expressed) Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Product Specifications for Recombinant Mouse IL-17A (CHO-expressed) Protein

Formulation, Preparation and Storage

Carrier Free

7956-ML

Carrier

7956-ML/CF

Carrier Free

Reconstitution Calculator

Background: IL-17/IL-17A

Citations for Recombinant Mouse IL-17A (CHO-expressed) Protein (3)

Customer Reviews for Recombinant Mouse IL-17A (CHO-expressed) Protein (1)

Recombinant Mouse IL-17A (CHO-expressed) Protein

Product Documents for Recombinant Mouse IL-17A (CHO-expressed) Protein

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected