Product Specifications for Recombinant Mouse IL-12 R beta 1 Protein, CF
Mouse myeloma cell line, NS0-derived mouse IL-12 R beta 1 protein Gln20-Glu561, with a C-terminal 6-His tag
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
No results obtained: Gln20 predicted
Predicted Molecular Mass
95-110 kDa, reducing conditions
Measured by its binding ability in a functional ELISA. Immobilized rmIL-12 R beta 1 at 10 µg/mL (100 µL/well) can bind rmIL-12 with a linear range of 0.3-20 µg/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-12 R beta 1
IL-12 R beta 1 is a 100 kDa type I transmembrane protein that belongs to the gp130/G-CSF R family of cytokine receptors. IL-12 R beta 1 is a common subunit of both the IL-12 and IL-23 receptor complexes which play distinct but related roles in T cell mediated inflammatory reactions (1, 2). Mature mouse IL-12 R beta 1 contains a 546 amino acid (aa) extracellular domain (ECD) with five fibronectin type III repeats, and a 147 aa cytoplasmic domain (3). Within the ECD, mouse IL-12 R beta 1 shares 85% and 52% aa sequence identity with rat and human IL-12 R beta 1, respectively. It shares 16% - 21% aa sequence identity with the ECDs of mouse gp130, LIF R, G-CSF R, and IL-23 R. IL-12 and IL-23 are disulfide linked heterodimeric cytokines that share a common p40 subunit (1, 2). IL-12 R beta 1 interacts with p40 at low affinity but does not transmit signals (3). Increased ligand binding affinity and signaling capacity are gained by association of IL-12 R beta 1 with either IL-12 R beta 2 or IL-23 R (4 - 6). IL-12 R beta 2 and IL-23 R are the signal transducing components of these receptor complexes (4, 7). IL-12 R beta 1 is expressed on activated T cells, NK cells, B cells, macrophages, and microglia (8 - 10). IL-12 induced signaling promotes the development of naïve T cells into IFN-beta producing Th1 cells (11). IL-23 contributes to chronic inflammation by inducing the production of IL-17 by memory T cells (12). Naturally occurring homodimers of p40 can function as antagonists of IL-12 and IL-23 and can also induce macrophage chemotaxis in the absence of IL-12 R beta 2 (13, 14).
Becker, C. et al. (2005) Inflamm. Bowel Dis. 11:755.
Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
Chua, A.O. et al. (1995) J. Immunol. 155:4286.
Parham, C. et al. (2002) J. Immunol. 168:5699.
Wu, C. et al. (1997) J. Immunol. 159:1658.
Zou, J. et al. (1997) J. Biol. Chem. 272:6073.
Presky, D.H. et al. (1996) Proc. Natl. Acad. Sci. 93:14002.
Wu, C. et al. (1997) Eur. J. Immunol. 27:147.
Airoldi, I. et al. (2000) J. Immunol. 165:6880.
Li, J. et al. (2003) J. Neurol. Sci. 215:95.
Schmitt, E. et al. (1994) Eur. J. Immunol. 24:793.
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