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Recombinant Mouse IL-10 (Cys167Tyr) Protein

Catalog # 1023-ML | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
1023-ML-010/CF

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With Carrier
1023-ML-010

Key Product Details

Source

E. coli

Accession #

Structure / Form

Noncovalently-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived mouse IL-10 protein
Ser19-Ser178 (Cys167Tyr), with an N-terminal Met

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser19

Predicted Molecular Mass

18.8 kDa (monomer)

SDS-PAGE

17 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507.
The ED50 for this effect is 0.4-1.2 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1023-ML
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1023-ML/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha-helical cytokines that also includes IL-19, IL‑20, IL-22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2-5). Mature mouse IL-10 shares 85% amino acid sequence identity with rat and 70%-77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8-10). The IL-10 dimer binds to two IL-10 R alpha/IL-10 R1 chains, resulting in recruitment of two IL-10 R beta/IL-10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL-10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (12-14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15-17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

References

  1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  2. O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425.
  3. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
  4. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
  5. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
  6. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
  7. Hsu, D.-H. et al. (1990) Science 250:830.
  8. Moore, K. et al. (1990) Science 248:1230.
  9. Windsor, W.T. et al. (1993) Biochemistry 32:8807.
  10. Syto, R. et al. (1998) Biochemistry 37:16943.
  11. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  12. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
  13. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
  14. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  15. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
  16. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
  17. Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143.

Long Name

Interleukin 10

Alternate Names

CSIF, GVHDS, IL10, IL10A, TGIF

Entrez Gene IDs

3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)

Gene Symbol

IL10

UniProt

Product Documents for Recombinant Mouse IL-10 (Cys167Tyr) Protein

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse IL-10 (Cys167Tyr) Protein

For research use only

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