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Recombinant Mouse IGFBP-1 Protein, CF

Catalog # 1588-B1 | R&D Systems, Inc. a Bio-Techne Brand
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1588-B1-025

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IGFBP-1 protein
Ala26-Asn272

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala26

Predicted Molecular Mass

27 kDa

SDS-PAGE

34 kDa, reducing conditions

Activity

Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.
The ED50 for this effect is typically 0.25-1.5 µg/mL in the presence of 2.5 ng/mL Recombinant Mouse IGF-I.

Formulation, Preparation and Storage

1588-B1
Formulation Supplied as a 0.2 μm filtered solution in Acetonitrile and TFA.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.

Background: IGFBP-1

The insulin-like growth factor binding protein (IGFBP) family consists of six structurally related proteins that bind IGF with high affinity (1, 2). These proteins share conserved cysteine-rich N- and C- terminal regions that participate in IGF binding. IGFBPs regulate the bioavailability of IGFs and modulate their biological activities, both positively and negatively. Some IGFBPs also have intrinsic bioactivity that is IGF-independent. Post-transitional modifications of the IGFBPs, including glycosylation, phosphorylation and proteolysis, influence IGF binding affinities and tissue localization, affecting both the IGF-dependent and independent functions (1, 2).

Mouse IGFBP-1 cDNA encodes a 272 amino acid (aa) residue precursor protein with a putative 25 aa signal peptide (3). Mature mouse IGFBP-1 contains potential phosphorylation and proteolytic cleavage sites, an Arg-Gly-Asp (RGD) integrin receptor recognition sequence, but lacks potential N-linked glycosylation sites.
IGFBP-1 binds equally well to IGF-I and IGF-II. Phosphorylation of human IGFBP-1, but not rat IGFBP-1, increases IGF affinity. Mouse IGFBP-1 shares 67% and 93% aa sequence identity with human and rat IGFBP-1, respectively. IGFBP-1 is expressed in liver, decidua, and kidneys and is the most abundant IGFBP in amniotic fluid. Hepatic production of IGFBP-1 is down-regulated by insulin and up-regulated by glucocorticoids. Circulating IGFBP-1 levels are elevated under various catabolic conditions including bacterial or viral infections, trauma and diabetes (4). IGFBP-1 has been shown to either potentiate or inhibit the activities of IGF in a variety of cells. IGFBP-1, through its RDG motif, also interacts with alpha5 beta1 intergrin to stimulate changes in cell adhesion and migration in the absence of IGFs (1, 2).

References

  1. Firth, S.M. and R.C. Baxter (2002) Endocrine Reviews 23:824.
  2. Rajaram, S. et al. (1997) Endocrine Reviews 18:801.
  3. Schuller, A.G. et al. (1994) Mol. Cell. Endocrinol. 104:57.
  4. Lang, C.H. et al. (2003) Endocrinology 144:3922.

Long Name

Insulin-like Growth Factor Binding Protein 1

Alternate Names

IGFBP1

Entrez Gene IDs

3484 (Human); 16006 (Mouse)

Gene Symbol

IGFBP1

UniProt

Product Documents for Recombinant Mouse IGFBP-1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse IGFBP-1 Protein, CF

For research use only

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